RESUMO
Plasma concentrations of 25-hydroxy-cholecalciferol (25-OHD3) and vitamin-D-binding globulin (V.D.B.G.) were significantly reduced in ten nephrotic subjects. V.D.B.G., which is undetectable in normal urine, was present in substantial amounts in the urine of each nephrotic subject. Administration of 3H-labelled cholecalciferol to three subjects resulted in the rapid appearance of the labelled vitamin in the urine, mainly as the 25-hydroxylated metabolite bound to V.D.B.G. in amounts which could largely account for the low plasma-25-OHD3. The plasma half-life of 25-OHD3 was substantially reduced in the nephrotic syndrome.
Assuntos
Síndrome Nefrótica/metabolismo , Vitamina D/metabolismo , Adulto , Idoso , Fosfatase Alcalina/sangue , Colecalciferol/metabolismo , Cromatografia/métodos , Feminino , Globulinas/urina , Meia-Vida , Humanos , Marcação por Isótopo , Masculino , Pessoa de Meia-Idade , Ligação Proteica , Proteinúria/urina , Albumina Sérica/análise , Soroglobulinas/análise , TrítioRESUMO
Erythrocyte carbonic-anhydrase-I (C.A.I.) concentration was measured by radial immunodiffusion in 30 thyrotoxic patients and 15 myxoedemic patients, the results being compared with those found in a control group of 90 euthyroid individuals. In thyrotoxicosis the erythrocyte-C.A.I. concentration was found to be below 10-29 mg/g haemoglobin, whereas the normal range varied from 11-1 to 22-5 mg C.A.I./g Hb. Low values were found in 4 patients with triiodothyronine(T3)-toxicosis. In myxoedema, the mean erythrocyte-C.A.I. concentration, though elevated at 20-37 mg/g Hb, fell within the normal range. In pregnancy, the erythrocyte-C.A.I. concentration rose with time, the mean value at tern being 20-6 +/-S.D. 3-3 mg/g Hb. In a group of women taking oral contraceptives, a low erythrocyte-C.A.I. concentration was noted--an effect which was most obvious among those taking low-oestrogen contraceptives. It is suggested that erythrocyte-C.A.I. estimation provdes a rapid screening test for thyrotoxicosis.
Assuntos
Anidrases Carbônicas/sangue , Eritrócitos/enzimologia , Hipertireoidismo/diagnóstico , Mixedema/diagnóstico , Adolescente , Adulto , Feminino , Humanos , Hipertireoidismo/sangue , Imunodifusão/métodos , Pessoa de Meia-Idade , Mixedema/sangue , Gravidez , Complicações na Gravidez/sangue , Complicações na Gravidez/diagnósticoRESUMO
An electrophoretic survey of variant red cell enzyme phenotypes in an isolated community in Newfoundland gave gene frequencies in marked contrast to those found in the capital, St. John's. In particular, a variant of red cell superoxide dismutase (SOD) gave a high variant frequency. Possible origins of the variant SOD allele are discussed.
