Assuntos
Vírus da Encefalite Transmitidos por Carrapatos/imunologia , Encefalite Transmitida por Carrapatos/prevenção & controle , Vacinas Virais/imunologia , Adolescente , Adulto , Animais , Anticorpos Antivirais/sangue , Relação Dose-Resposta Imunológica , Avaliação de Medicamentos , Avaliação Pré-Clínica de Medicamentos , Humanos , Imunização , Camundongos , Fatores de Tempo , Ultrafiltração , Vacinas de Produtos Inativados/administração & dosagem , Vacinas de Produtos Inativados/efeitos adversos , Vacinas de Produtos Inativados/imunologia , Vacinas de Produtos Inativados/isolamento & purificação , Vacinas Virais/administração & dosagem , Vacinas Virais/efeitos adversos , Vacinas Virais/isolamento & purificação , Cultura de Vírus/métodosRESUMO
Two strains of diploid fibroblasts were derived from green monkey fetal lungs and their main properties were studied. These strains were shown not to differ principally from the well studied human diploid fibroblasts. The derived cultures are free from contaminating viruses. The titers of attenuated poliomyelitis virus strains achieved in green monkey diploid cells are comparable to those in primary cultures of green monkey kidneys. The possiblity of using green monkey diploid fibroblasts in virological practice is discussed.
Assuntos
Linhagem Celular , Poliovirus/crescimento & desenvolvimento , Cultura de Vírus , Animais , Callitrichinae , Cromossomos , Diploide , Fibroblastos , Congelamento , CariotipagemAssuntos
Antivirais/isolamento & purificação , Vacinas/isolamento & purificação , Animais , Callitrichinae , Células Cultivadas , Efeito Citopatogênico Viral , Haplorrinos , Vacina Antipólio Oral/análise , Vacina Antipólio Oral/isolamento & purificação , Vacina Antipólio Oral/normas , Codorniz , Virulência , Cultura de VírusRESUMO
Differences between strains of measles virus in their mutagenic effect on cells of different animals and man were found. The mutagenic activity of measles virus strains was species-specific. In the process of spontaneous transformation the sensitivity of cells to the mutagen changed.
Assuntos
Aberrações Cromossômicas , Efeito Citopatogênico Viral , Vírus do Sarampo , Mutação , Animais , Linhagem Celular , Cricetinae , Humanos , Ratos , Ovinos , Especificidade da EspécieRESUMO
The intensity of autoantibody production to red blood cell lysate in guinea pigs inoculated with smallpox vaccine prepared from different strains was studied at 7, 14, 21 and 28 days after vaccine inoculation. The dynamics of autoantibody production in the same animals as well as the influence of altered responsiveness of the animals pre-treated with dyphtheria toxin on the intensity of autoantibody production were determined. The autoantibody-inducing activity correlated with reactogenicity of the strains and was most marked in the white cloned variant of the Tashkent strain, slightly lower in the L-IVP strain being intermediate in this criterion and insignificant in poorly reactogenic EM-63 and B-51 strains. In the animals with altered responsiveness the autoimmune responses were increased. The results of this study were tested statistically.
