RESUMO
A total of 212 coagulase-negative Staphylococcus strains recovered prospectively during 119 surgeries for proven or suspected bone and joint infection (BJI) were identified by sodA sequencing. These strains were identified as 151 Staphylococcus epidermidis isolates, 15 S. warneri isolates, 14 S. capitis isolates, 9 S. hominis isolates, 6 S. lugdunensis isolates, 5 S. haemolyticus isolates, 4 S. caprae isolates, 4 S. pasteuri isolates, 3 S. simulans isolates, and 1 S. cohnii isolate. Only S. epidermidis, S. lugdunensis, S. capitis, and S. caprae were found to be infecting organisms and were involved, respectively, in 35 (81.4%), 3 (7.0%), 3 (7.0%), and 2 (4.6%) cases of BJI.
Assuntos
Proteínas de Bactérias/genética , Doenças Ósseas Infecciosas/cirurgia , Coagulase/metabolismo , Artropatias/cirurgia , Infecções Estafilocócicas/microbiologia , Staphylococcus/classificação , Staphylococcus/isolamento & purificação , Superóxido Dismutase/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Artroplastia/efeitos adversos , Técnicas de Tipagem Bacteriana , Doenças Ósseas Infecciosas/microbiologia , Feminino , Genótipo , Humanos , Artropatias/microbiologia , Masculino , Pessoa de Meia-Idade , Ortopedia/métodos , Estudos Prospectivos , Reoperação , Staphylococcus/enzimologia , Staphylococcus/genéticaRESUMO
This study evaluated the possible advantages provided by a genotypic method over commercially available biochemical systems for the identification of clinical isolates of coagulase-negative staphylococci (CNS). Partial sequencing of the sodA gene was performed for 168 coagulase-negative clinical isolates of staphylococci identified previously with the ID32 STAPH system. Of these, 101 (60.1%) were identified to the species level with ID32 STAPH, while 67 (39.9%) were misidentified or not identified with certainty. Sequencing of sodA proved useful for resolving all ambiguities or inconclusive identifications generated by the commercially available biochemical identification system.
