Distribution of lactate dehydrogenase isoenzymes in normal and inflamed bovine udders and milk.

The patterns of distribution of lactate dehydrogenase (LDH) isoenzymes were determined in normal and inflamed bovine udder tissues, in normal and mastitic milk-leucocytes and serum. LDH1 was the most common isoenzyme found in all the types of tissues examined, normal tissues contained the lowest proportions of LDH5 whereas the inflamed tissues and leucocytes from mastitic milk showed a higher proportion of LDH4 and LDH5. It seems that the origin of the elevated LDH in mastitic milk is the leucocytes and the parenchyma cells of the udder. The significance of the shift in the molecular forms of LDH isoenzymes is discussed in relation to possible alternations in energy metabolism in the inflamed bovine mammary gland.

Changes in the biochemical composition of blood in chickens infected with Borrelia anserina.

Three-month-old chickens were inoculated intramuscularly with blood infected with Borrelia anserina. The serum levels of the enzymes alkaline and acid phosphatases (ALP and AcP), glutamate-oxaloacetate transaminase (GOT), proteins, lipids, uric acid, blood sugar and inorganic phosphate were measure. There was a decrease of ALP and increase of GOT levels in the infected group. The level of AcP was unchanged. The spirochaetosis was also accompanied by an increase of total protein, globulin and uric acid, reduction of albumin, total lipids, cholesterol and a slight decrease of inorganic phosphate. Blood sugar level was unchanged. These changes corresponded to the clinical and the post-mortem findings following avian spirochaetosis.