Assessment of the impact of co-curricular activities on achievement of Doctor of Pharmacy program outcomes.

INTRODUCTION: Co-curricular activities are recognized as an increasingly important aspect of pharmacy education. However, the impact of these activities on student learning is not well understood compared to that of curricular learning. The purpose of this study was to assess student-perceived progress in achieving program outcomes through voluntary co-curricular activities compared with learning of the same outcomes through mandatory curricular activities. METHODS: The study was performed over six semesters between fall 2017 and spring 2020 at the University of Southern California School of Pharmacy. Separate surveys were sent to all first- through third-year doctor of pharmacy students each semester to assess the impact of curricular and co-curricular activities on improvement in six program outcomes. Graduating student survey data were also mapped to learning outcomes to assess improvement of these outcomes upon graduation. RESULTS: Three main results emerged from these data. First, there was greater variation in the impact of co-curricular activities on different learning outcomes compared to the effect of curricular activities on the same outcomes. Second, co-curricular activities had a greater impact on "soft skills," including leadership and professionalism, compared to concrete knowledge in areas such as therapeutic mechanisms. Finally, the impact of co-curricular activities on most learning outcomes diminished with progression through the curriculum while the impact of curricular activities remained relatively constant. CONCLUSIONS: Student-perceived improvement in learning of program outcomes differs when based on co-curricular compared to curricular activities. These results show how these activities can complement each other in achievement of program outcomes.

An optimized microfluidic paper-based NiOOH/Zn alkaline battery.

In this paper, an alkaline nickel oxide hydroxide/zinc (NiOOH/Zn) battery featuring a cellulose matrix separator between electrodes is presented. The metallic electrodes and the paper separator are inserted in a layer-by-layer assembly that provides mechanical stability to the system resulting in a lightweight and easy-to-use device. The battery was optimized for the amount of NiOOH-ink used at the cathode (11.1 mg/cm2 ) and thickness of the paper membrane separating the electrodes (360 µm). The battery was able to function using a small volume (75 µL) of 1.5 M potassium hydroxide (KOH) producing a maximum voltage, current density, and power density of 1.35 ± 0.05 V, 10.62 ± 0.57 mA/cm², and 0.56 ± 0.01 mW/cm², respectively. The system displayed a maximum current of 23.9 mA and a maximum power of 1.26 mW. Moreover, four batteries connected in series were able to power a small flameless candle for approximately 22 min. This work has potential in fulfilling the demands for short-term and lightweight power supplies.

Use of chemometrics to optimize a glucose assay on a paper microfluidic platform.

We describe the use of a chemometrics-based computational platform to optimize a glucose assay on a microfluidic paper-based analytical device (µPAD). Glucose is colorimetrically detected in the presence of glucose oxidase (GOx), horseradish peroxidase (HRP), and potassium iodide (KI). Using a Y-shaped paper microfluidic chip, the concentration of glucose, volume of reagents, and the length and width of the microfluidic channel were examined. The responses of the microfluidic chips were analyzed at the halfway point of the channel length. Variables affecting the response were screened by using a 24 factorial design, and among them, volume and concentration of the glucose were optimized by applying a rotatable central composite design (CCD). The optimum and experimental responses are 151.58 and 149.80, respectively, with an absolute error of 1.2%.

An Inexpensive Paper-Based Aluminum-Air Battery.

Paper-based batteries are an alternative to traditional batteries due to their low cost, portability, and simplicity to operate. In the present work, we demonstrate an improved and inexpensive paper-based aluminum-air battery employing KOH as the electrolyte with sufficient energy to power small devices. The dimensions of the device, electrode size, and electrolyte concentration were optimized with respect to amperage and reproducibility. The maximum amperage of 17.4 mA and maximum power of 3.0 mW was achieved with a 9 cm² battery with anode and cathode electrode areas of 5.1 cm² and 3.75 cm² respectively, using 1.5 M potassium hydroxide (KOH). In a series configuration, the batteries generate sufficient energy to power light-emitting diodes (LEDs), a flashlight, a glucometer, and a pregnancy test.

Easily Fabricated Microfluidic Devices Using Permanent Marker Inks for Enzyme Assays.

In this communication, we describe microfluidic paper analytical devices (µPADs) easily fabricated from commercially available Sharpie ink permanent markers on chromatography paper to colorimetrically detect glucose using glucose oxidase (GOx). Here, solutions of horseradish peroxidase (HRP), GOx, and potassium iodide (KI)were directly spotted onto the center of the µPAD and flowed into samples of glucose that were separately spotted on the µPAD. Using an XY plotter (Roland DGA Corporation, Irvine, CA USA), several ink marks drawn in the paper act as the hydrophobic barriers, thereby, defining the hydrophilic fluid flow paths of the solutions. Two paper devices are described that act as independent assay zones. The glucose assay is based on the enzymatic oxidation of iodide to iodine whereby a color change from clear to brownish-yellow is associated with the presence of glucose. In these experiments, two designs are highlighted that consist of circular paper test regions fabricated for colorimetric and subsequent quantification detection of glucose. The use of permanent markers for paper patterning is inexpensive and rapid and does not require special laboratory equipment or technical skill.

Development of a microfluidic-based assay on a novel nitrocellulose platform.

A novel microfluidic paper-based analytical device (µPAD) utilizing a nitrocellulose (NC) membrane to detect IgG antibodies through a colorimetric analysis is described. The µPAD was constructed using layered polyethylene terephthalate (PET) and pressure-sensitive adhesives (PSA). The biotin labeled Goat Anti-Mouse IgG antibody was spotted and dried on the NC channel prior to subjecting it to a series of wash solutions (Tris-tween), increasing concentrations of alkaline phosphatase conjugated to streptavidin (Strep-ALP), and para-nitrophenyl phosphate (p-NPP) realizing a vibrant yellow color. The reaction proceeds for 10 min before applying the p-NPP stop solution. The device was then dried, scanned, and analyzed yielding a linear range of inverse yellow color intensities versus Strep-ALP concentrations. The development of this simple µPAD should further facilitate the use of NC in colorimetric assays to detect and quantitate antibodies.