RESUMO
The minor form of valine tRNA from baker's yeast-tRNAVal 2b--purified by column chromatography was completely digested with guanylo-RNase and pancreatic RNase. The products of these digestions were separated by a combination of thin-layer chromatography on cellulose and high voltage electrophoresis on DEAE-paper and then identified. The halves of tRNA Val 2b were prepared by partial digestion with pancreatic RNase, and their complete guanylo-RNase and pancreatic RNase digests were analysed. Basing on the obtained data the primary structure of baker's yeast tRNA Val 2b was reconstructed.
Assuntos
RNA de Transferência , Sequência de Bases , Oligorribonucleotídeos/análise , Pâncreas/enzimologia , RNA de Transferência/isolamento & purificação , Ribonuclease T1 , Ribonucleases , Ribonucleotídeos/análise , Saccharomyces cerevisiae/análise , ValinaRESUMO
A modified procedure for the separation of oligoribonucleotides is described that is based on the combination of t.l.c. on cellulose and electrophoresis on DEAE-paper at 4000 V on a cooling plate. The technique is relatively rapid and allows the analysis of larger quantities than is possible by electrophoresis on cellulose acetate.
