Microcotyle justinei n. sp. (Monogenea: Microcotylidae) from the Gills of the Cardinal Fish Apogon imberbis (Teleostei: Apogonidae) off the Algerian Coast of the Western Mediterranean.

PURPOSE: A new monogenean Microcotyle justinei n. sp. (Monogenea: Microcotylidae) is described based on specimens found on the gill filaments of the cardinal fish Apogon imberbis (Apogonidae) off the Algerian coast of the Western Mediterranean. METHODS: Monogeneans were examined, measured and drawn for a comparative morphological study with other species of Microcotyle and characterised molecularly using a partial fragment of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. The identification of fish was confirmed by molecular barcoding using the cox1 gene. RESULTS: The new species is distinguished from all other species of the genus by a combination of features, such as the number and size of the clamps, the shape and size of the genital atrium and the number of testes. The molecular analysis of the cox1 gene sequences showed that interspecific differences between Microcotyle justinei n. sp. and published sequences of Microcotyle spp. was greater than 8.8%, strongly suggesting that the new species is distinct from other congeners with sequences available on GenBank. CONCLUSION: The morphological and molecular analyses support the status of M. justinei as a new species. The present finding extends the list of Microcotyle spp. to 72.

Redescription, complete mitochondrial genome and phylogenetic relationships of Hexostoma thynni (Delaroche, 1811) Rafinesque, 1815 (Monogenea, Hexostomatidae).

Specimens of Hexostoma thynni (Delaroche, 1811) Rafinesque, 1815 were collected from their type-host, the bluefin tuna Thunnus thynnus, caught off Algeria, i.e. close to the type-locality, off Mallorca, which is also in the Mediterranean. The species is briefly redescribed and compared to previous descriptions, under the same name or as its synonym Plagiopeltis duplicata Diesing, 1858, to ascertain identity of specimens. The three genera within the Hexostomatidae (Hexostoma Rafinesque, 1815, Neohexostoma Price, 1961 and Homostoma Unnithan, 1965) are briefly discussed, with comments on the fragility of characters used to distinguish them. Using next-generation sequencing, the complete mitogenome and the cluster of ribosomal genes (SSU, LSU, ITS1, ITS2, 5.8S) were obtained. The mitogenome is 14,649 bp long and codes for 12 protein-coding genes, 2 ribosomal RNA genes and 22 transfer RNA genes; its size is similar to other mitogenomes obtained from polyopisthocotylean monogeneans. A phylogeny based on concatenated mitogenome protein-coding genes from nine species of polyopisthocotylean monogeneans produced a tree in which the Hexostomatidae H. thynni was associated with other Mazocraeidea, such as Chauhaneidae and Diclidophoridae. This invalidates the hypothesis of Boeger & Kritsky (1993) of Hexostomatidae as sister-group to the Mazocraeidea and suggests the demise of the suborder Hexostomatinea Boeger & Kritsky, 1993. We insist on the usefulness of depositing parts of specimens used for molecular analyses, prepared on permanent slides, in a curated collection.

Title: Redescription, génome mitochondrial complet et relations phylogénétiques d'Hexostoma thynni (Delaroche, 1811) Rafinesque, 1815 (Monogenea, Hexostomatidae). Abstract: Des spécimens d'Hexostoma thynni (Delaroche, 1811) Rafinesque, 1815 ont été collectés sur leur hôte-type, le thon rouge Thunnus thynnus, capturé au large de l'Algérie, c'est-à-dire près de la localité-type, au large de Majorque, qui se trouve également en Méditerranée. L'espèce est brièvement redécrite et comparée aux descriptions précédentes, sous le même nom ou sous son synonyme Plagiopeltis duplicata Diesing, 1858, pour vérifier l'identité des spécimens. Les trois genres au sein des Hexostomatidae (Hexostoma Rafinesque, 1815, Neohexostoma Price, 1961 et Homostoma Unnithan, 1965) sont brièvement discutés, avec des commentaires sur la fragilité des caractères utilisés pour les distinguer. En utilisant le séquençage de nouvelle génération, le mitogénome complet et le groupe de gènes ribosomiques (SSU, LSU, ITS1, ITS2, 5.8S) ont été obtenus. Le mitogénome a une longueur de 14 649 pb et code pour 12 gènes codant pour des protéines, 2 gènes d'ARN ribosomal et 22 gènes d'ARN de transfert, et sa taille est similaire à celle des autres mitogénomes obtenus de monogènes Polyopisthocotylea. Une phylogénie basée sur les gènes codant pour les protéines concaténées du mitogénome de 9 espèces de monogènes Polyopisthocotylea a produit un arbre dans lequel l'Hexostomatidae H. thynni était associé à d'autres Mazocraeidea tels que les Chauhaneidae et les Diclidophoridae. Ceci réfute l'hypothèse de Boeger & Kritsky (1993) des Hexostomatidae comme groupe-frère des Mazocraeidea et suggère la disparition du sous-ordre Hexostomatinea Boeger & Kritsky, 1993. Nous insistons sur l'intérêt de déposer dans une collection entretenue des parties des spécimens utilisées pour les analyses moléculaires, préparées sur des lames microscopiques permanentes.

A revision of Plectanocotyle (Monogenea, Plectanocotylidae), with molecular barcoding of three species and the description of a new species from the streaked gurnard Chelidonichthys lastoviza off Algeria.

BACKGROUND: The family Plectanocotylidae includes parasites of the gills of marine fish; although nine genera and about 20 species have been described, almost no molecular information is available. Putting aside Plectanocotyle elliptica Diesing, 1850, supposedly a parasite of the white perch Morone americana, never found again since its original description, two species were valid within Plectanocotyle Diesing, 1850 before this work: Plectanocotyle gurnardi (Van Beneden & Hesse, 1863) Llewellyn, 1941 and Plectanocotyle major Boudaya, Neifar & Euzet, 2006. METHODS: In this paper, we describe the third species of the genus Plectanocotyle and perform a comparative morphological and molecular analysis of the three species and of Triglicola obscura (Euzet & Suriano, 1974) Mamaev, 1976. Host fishes were also barcoded (COI) for confirmation of host identifications. RESULTS: Plectanocotyle lastovizae n. sp. is described from the gills of the streaked gurnard Chelidonichthys lastoviza collected off Algeria. The species is compared with specimens of Plectanocotyle cf. gurnardi (from C. lastoviza) from the same locality and P. major and T. obscura (both from the longfin gurnard C. obscurus). Molecules from Plectanocotyle cf. gurnardi could not be compared with P. gurnardi from the type-host and type-locality and we kept the status of the Mediterranean specimens as pending. Algeria is a new geographic record for P. major and T. obscura. Plectanocotyle lastovizae n. sp. is distinguished from the other species found in the Mediterranean by the measurements of clamps, number of testes, and COI sequences, with notable divergence (7.8-11.8%) from the other two species of the genus. DISCUSSION: We briefly present a list of currently known members of the family Plectanocotylidae, their biology and their hosts.

A new species of Microcotyle (Monogenea: Microcotylidae) from Scorpaena notata (Teleostei: Scorpaenidae) in the Mediterranean Sea.

We collected specimens of Microcotyle spp. from two species of scorpaeniform fishes off Algeria, namely Scorpaena notata and Helicolenus dactylopterus. The identification of both fishes was confirmed by molecular barcoding of the COI gene. Sequences of COI gene were also obtained for both parasite species. The species from S. notata is described as Microcotyle algeriensis n. sp., on the basis of morphological differences from other species (number of clamps, number of spines in genital atrium, number of testes). Its COI sequence differs from M. sebastis Goto, 1894 (from Sebastes schlegeli from a fish farm in South Korea) by 14.6%. The species from H. dactylopterus is distinct from M. algeriensis on the basis of morphology (number of clamps, number of spines in genital atrium) and COI sequence (4.5% divergence) and is also distinct from M. sebastis in its COI sequence (12.3%). We refrained from describing it as new because M. sebastis, a species originally described from scorpaeniform fishes off Japan, has been recorded in various hosts in the North and South Pacific, Atlantic and Mediterranean (for the latter, in the same host, H. dactylopterus). We believe that correct specific assignment of species of Microcotyle from scorpaeniform fishes needs a detailed morphological and molecular study of representatives from various locations and hosts.