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1.
Vet Immunol Immunopathol ; 197: 15-20, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29475501

RESUMO

The mosquito-borne Rift Valley fever virus (RVFV) causes severe diseases in domesticated animals including cattle, sheep, camels and goats. Capripoxviruses (CPV) are suitable vectors for multivalent vaccine development. A recombinant rKS1-based CPV expressing the gene encoding the viral glycoprotein Gn of RVFV has been shown to induce protection in mice and sheep. The aim of this study was to evaluate the immunogenicity induced by this candidate vaccine in goats, and the level of cytokines produced by RVFV-specific Th1 and Th2 lymphocytes. The results of this study suggest that Th2 mediates immunity mainly through the significant production of IL4, which, coupled with a decrease in IFN-γ, may be involved in the replication of the capripoxvirus expressing the GN of RVFV. CD4+ cells may play the role of helper cells in B cell responses and neutralizing antibody production in the anti-CPV humoral response, leading to strong immunity against RVFV.


Assuntos
Citocinas/imunologia , Febre do Vale de Rift/prevenção & controle , Vacinação/veterinária , Proteínas do Envelope Viral/imunologia , Vacinas Virais/imunologia , Animais , Anticorpos Neutralizantes/sangue , Anticorpos Antivirais/sangue , Capripoxvirus , Cabras , Imunogenicidade da Vacina , Proteínas Recombinantes/imunologia , Febre do Vale de Rift/imunologia , Vírus da Febre do Vale do Rift , Vacinas Virais/genética
2.
J Gen Virol ; 93(Pt 7): 1456-1464, 2012 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-22513390

RESUMO

Currently, there are no worldwide licensed vaccines for Rift Valley fever (RVF) that are both safe and effective. Development and evaluation of vaccines, diagnostics and treatments depend on the availability of appropriate animal models. Animal models are also necessary to understand the basic pathobiology of infection. Here, we report the use of an inbred MBT/Pas mouse model that consistently reproduces RVF disease and serves our purpose for testing the efficacy of vaccine candidates; an attenuated Rift Valley fever virus (RVFV) and a recombinant RVFV-capripoxvirus. We show that this model is relevant for vaccine testing.


Assuntos
Modelos Animais de Doenças , Avaliação Pré-Clínica de Medicamentos/métodos , Febre do Vale de Rift/imunologia , Febre do Vale de Rift/prevenção & controle , Vírus da Febre do Vale do Rift/imunologia , Vacinação/métodos , Vacinas Virais/imunologia , Animais , Feminino , Humanos , Camundongos , Vacinas Atenuadas/administração & dosagem , Vacinas Atenuadas/imunologia , Vacinas Virais/administração & dosagem
3.
Vet J ; 187(3): 402-4, 2011 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20167519

RESUMO

This study, carried out between September 2006 and January 2007, is the first cross-sectional serological investigation of peste-des-petits-ruminants (PPR) and Rift Valley fever (RVF) in Tunisia. The objective was to assess the potential need to develop a dual, recombinant PPR-RVF vaccine and how such a vaccine might be utilised in Tunisia. An overall PPR seroprevalence of 7.45% was determined, a finding supported by the high specificity (99.4%) and sensitivity (94.5%) of the ELISA used. On assessment of the diversity and density of mosquitoes in the sampling area, four species of RVF-vectors of the genus Aedes and Culex were identified. However, no serological evidence of RVF was found despite the use of a highly sensitive ELISA (99-100%). Larger scale investigations are underway to confirm these findings and the continuation of the emergency vaccination program against these two diseases remains valid.


Assuntos
Anticorpos Antivirais/sangue , Doenças das Cabras/epidemiologia , Peste dos Pequenos Ruminantes/veterinária , Febre do Vale de Rift/veterinária , Doenças dos Ovinos/epidemiologia , Animais , Estudos Transversais , Vetores de Doenças , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Cabras , Masculino , Peste dos Pequenos Ruminantes/epidemiologia , Vírus da Peste dos Pequenos Ruminantes/imunologia , Febre do Vale de Rift/epidemiologia , Vírus da Febre do Vale do Rift/imunologia , Estudos Soroepidemiológicos , Ovinos , Tunísia/epidemiologia
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