Effects of essential oil mixtures on expression of genes involved in lipogenesis and fatty acid oxidation in geese (Anser anser).

The use of essential oils has recently increased in the poultry sector. The aim of this study was to investigate the effects of essential oil mixture (juniper, mint, oregano and rosemary oil) on fatty acid oxidation and lipogenic gene expression in geese. Research groups were formed as C (control; no additives), EK1 (0.4 ml/l essential oil mixture supplemented) and EK2 (0.8 ml/l essential oil mixture supplemented). Relative expression levels of genes included in lipogenesis (ACCα, ChREBP, FASN, LXRα and SREBP-1) expression levels of genes included in fatty acid oxidation (ACOX1, CPT1, CPT1A, PPARα and PPARγ) were measured using RT-qPCR. Group EK1 upregulates the mRNA expression levels of genes involved in lipogenesis such as ACCα, ChREBP and SREBP-1, while it downregulates the mRNA expression in levels of all genes involved in fatty acid oxidation. Group EK2 increases the mRNA expression levels of genes involved in lipogenesis such as ACCα, FASN and SREBP-1, while it decreased mRNA expression at the levels of all genes involved in fatty acid oxidation, as in the other group. In the study, adding an essential oil mixture to drinking water is predicted to increase fatty liver because it upregulates genes related to fat synthesis (lipogenesis) and downregulates genes related to fat degradation (fatty acid oxidation).

Effects of bergamot (Citrus bergamia) peel oil-supplemented diets on growth performance, haematology and serum biochemical parameters of Nile tilapia (Oreochromis niloticus).

The present study investigated the effects of dietary Citrus bergamia peel oil (CBO) on growth performance, haematological and serum biochemical responses of Nile tilapia, Oreochromis niloticus. Volatile content of C. bergamia peel oil used in study was detected with GC/MS. The 99.47% of the analysed volatile components were identified in decreasing quantities as limonene, linalool and linalyl acetate. The experimental fish were divided into four groups stocked into triplicate tanks (20 fish/tank), and fed daily with an additive-free basal diet containing 0% (CBO0), 0.5% (CBO0.5), 1.0% (CBO1), 2.0% (CBO2) of bergamot peel oil for an 8-week period. Results showed that dietary bergamot peel oil especially at 0.5 g 100 g-1 significantly increased growth performance of fish (p < 0.05). The levels of haemoglobin and haematocrit significantly increased in fish fed with CBO0.5 and CBO1 compared with the CBO0-fed group (p < 0.05), despite no significant difference was observed in CBO2 group (p > 0.05). Highest haematocrit value was found in the CBO0.5 group compared with the control group. The serum glucose of the fish fed with CBO0.5 and CBO1 feeds decreased significantly compared with the control group (p < 0.05). The highest total protein, lowest cholesterol and triglyceride in serum were observed in fish fed with bergamot oil-supplemented groups. According to the results, it was revealed that 0.5% bergamot oil supplementation to Nile tilapia diets optimizes the growth performance, feed utilization and health status related with blood parameters of the fish.