Antioxidant and Antimicrobial Properties of Stachys maritima via Quantum Dots and Molecular Docking.

The antioxidant activity of Stachys maritima was determined via initial tests, yielding a DPPH IC50 value of 0.114 ± 0.001 mg/mL, indicative of its ability to neutralize free radicals and mitigate oxidative stress. Moreover, the plant extract demonstrated a significant iron chelation capacity comparable to EDTA (IC50: 5.98 ± 0.10 mg/mL). Analysis of the extract revealed high levels of flavonols, flavonoids, phenolic compounds, proanthocyanidins, and tannins, notably totaling 1039.48 ± 6.53 mg GAE/g and 276.21 ± 4.38 mg CAE/g, respectively, contributing to its robust antioxidant potential. ST-CQDs, nanoscale carbon structures, have garnered interest in biomedicine for their antioxidant properties. Confirmatory analyses showed their high antioxidant activity with DPPH IC50 value of 0.69 ± 0.03 mg/mL and iron chelation capacity of 3.86 ± 0.41 mg/mL. Antimicrobial tests revealed S. maritima efficacy against various pathogens, while ST-CQDs showed no effect. High peak areas in analyses suggest specific compounds' presence, notably certain fatty acids, highlighting their crucial role in composition understanding. Notably, 9,12,15-Octadecatrienoic acid's importance is underscored. These findings provide a significant basis for further exploring TNF development's molecular mechanisms.

Phytochemical Analysis of Anacamptis coriophora Plant Cultivated Using Ex Vitro Symbiotic Propagation.

This study aims to investigate the functional groups and phytochemical profile of Anacamptis coriophora seeds, tubers, and flowers. Symbiotic seedlings produced using the ex vitro method were transferred to their natural habitat and grown to analyze the functional groups and phytochemical profiles of tubers and flowers. The life cycles of the transferred seedlings were monitored, and tubers and flowers were harvested for analysis. ATR-FTIR spectroscopy revealed the presence of functional groups such as polysaccharides, lignin, and proteins in both tubers and flowers. Differences in spectral frequencies between first-year and second-year tubers were observed. Fatty acid analysis identified 30 different compounds in seeds, flowers, and tubers, with linoleic acid being the most abundant (27 % in seeds, 33 % in tubers), and palmitic acid present in flowers (24 %). GC-MS analysis of ethanol extracts from these components highlighted the presence of 32 compounds, including hydroxyacetic acid, hydrazide, cytidine (Z)-7-hexadecenal, 2,2-dimethoxyethane, 2,5,6-trimethyldecane, and butanamide, 4-amino-N-hydroxy. A. coriophora's tubers, flowers, and seeds may contain active metabolites with therapeutic potential. These results are valuable for the commercial cultivation of the plant.