Fine needle aspiration cytology of high grade mucoepidermoid carcinoma of the breast: a case report.

BACKGROUND: Primary mucoepidermoid carcinoma of the breast is a very unusual tumor. It is often misdiagnosed, masquerading under different diagnoses. The cytologic assessment is especially difficult when the lesion is high grade. One reported case was initially diagnosed by fine needle aspiration cytology. CASE: A 69-year-old woman presented with a 6 x 4-cm tumor located in the upper outer quadrant of the right breast. The first cytologic diagnosis suggested ductal carcinoma with atypical squamous metaplasia; further review disclosed that the clusters of epithelial ductal cells displayed a mixed pattern of glandular, squamous and intermediate cells. There also was a scant intracellular and extracellular mucous substance, confirming the diagnosis of mucoepidermoid carcinoma. Histochemistry and immunohistochemistry, performed on the tumor and lymph node metastases, showed cellular staining for periodic acid-Schiff, and keratin, epithelial membrane antigen and carcinoembryonic antigen demonstrated the epithelial origin. The high expression of Ki-67, as well as the finding of 24 metastasized nodes in the axilla, demonstrated the tumor's aggressiveness. CONCLUSION: Fine needle aspiration cytology is a very reliable tool in achieving a fast and accurate diagnosis of primary mucoepidermoid carcinoma of the breast.

DNA quantification as prognostic factor in a case of acinar cell carcinoma of the parotid gland, diagnosed by FNA.

Hereby we present a case of a 43-years-old male who complained of a three years history preauricular painful mass. Fine needle aspiration cytology was performed, diagnosing of compatible with acinar cell carcinoma, thus DNA quantification by image cytometry was carried out. Biological parameters studied (ploidy, S-phase, 5-c exceeding rate) showed that it is a low grade of malignancy lesion. Total parotidectomy conservative of facial nerve was recommended, without regional lymphadenectomy. Patient remains, one year later, asymptomatic and free of disease.

Cuantificación de ADN como factor pronóstico en un caso de Carcinoma de células Acinares de parótida diagnosticado por PAAF / DNA quantification as prognostic factor in a case of Acinar Cell Carcinoma of the parotid gland, diagnosed by FNA

Presentamos el caso de un varón de 43 años de edad que consulta por una tumoración preauricular derecha dolorosa a la palpación que, según refiere, tiene más de tres años de evolución. Se realiza punción aspiración con aguja fina que informa de compatible con carcinoma de células acinares, por lo que se realiza cuantificación de ADN por citometría de imagen. Los parámetros biológicos estudiados (ploidía, fase S, células por encima de 5c) indican que se trata de una lesión de bajo grado de malignidad.Con estos datos se practica parotidectomía total conservadora del facial, sin vaciamiento ganglionar. El paciente se encuentra, un año después, asintomático y libre de signos tumorales residuales

Hereby we present a case of a 43-years-old male who complained of a three years history preauricular painful mass. Fine needle aspiration cytology was performed, diagnosing of compatible with acinar cell carcinoma, thus DNA quantification by image cytometry was carried out. Biological parameters studied (ploidy, S-phase, 5-c exceeding rate) showed that it is a low grade of malignancy lesion. ;;Total parotidectomy conservative of facial nerve was recommended, without regional lymphadenectomy. Patient remains, one year later, asymptomatic and free of disease

[Descriptive study of HPV prevalence in a private consultation]. / Estudio descriptivo de la prevalencia de HPV en una consulta privada.

Human papillomavirus (HPV) infection is the main aethiological factor in the development of squamous cervical neoplasia. Nowadays molecular hybridization is the most adequated method to detect HPV. Study is based on the HPV assay by means of mixed hybridization and capture of specific sequences of viral DNA. Detection of HPV by hybridization and capture is a very useful technique in patients with non-definitive cytologies of viral action, but that display high clinical suspect for such a infection. The cervical cancer screening increases its sensibility and specificity by a combined study of cytology and hibridization. The highest benefit is for those cases classified initially as ASCUS, in which the HPV results point out a more accurate diagnosis and guide subsequent adequate therapy. The results show that for normal cytology up to 87.1% was negative for virus, in the ASCUS group a 56.76% was positive for HPV, in the 32 LSIL cases a 43.8% was negative for HPV, while only a 33% of the HSIL II was negative, finally there were no negative cases for HSIL III, finding an 11.1% for low risk and low plus high risk, and a 77.8% for high risk alone. In spite of the clear relationship between cytology and HPV determination, one must be careful in establishing the treatment for the cases that displayed high risk HPV with cytology within normal limits or ASCUS. We consider these patients as high risk ones thus being suitable for a more strict follow up, keeping more aggressive procedures for the cases with cytologic manifestations.