[MAJOR MOLECULAR AND CELLULAR MANIFESTATIONS OF ATHEROSCLEROSIS].

The authors consider machanisms and peculiarities of development of atherosclerosis at the tissue level and the main characteristics of atherogenesis, viz. lipoidosis, fibrosis, enhanced intimacyte proliferation, local intima-media thickening. Results of counting subendothelial cells, assessment of their proliferative and synthetic activities in affected and intact arterial segments are presented Variants of lipid metabolism disorders are described.

[Prediction of peptide retention volumes in the gradient reversed phase HPLC].

A method of computation of retention volumes of linear peptides of known composition that contain no more than 25 amino acids in gradient reversed phase HPLC was developed. The method is suitable for various acetonitrile gradient profiles. The calculations were carried out on the basis of a statistical model, the parameters of which were experimental dependences of the retention of individual amino acids on acetonitrile concentration. The method developed was used to predict the chromatographic behavior of 34 peptides in four different acetonitrile gradients. The correlation coefficients between the predicted and experimental retention volumes were more than 0.9, and the average relative error of prediction was less than 15%. The English version of the paper: Russiatn .lournal of Bioorganic Chemistry, 2008, vol. 34, no. 2; see also http://www.maik.ru.

[Prediction of retention volumes and UV spectra of peptides in reversed phase HPLC].

A method for calculating retention volumes of linear peptides with known primary structures and the values of their UV absorption at chosen wavelengths in reversed phase HPLC are described. These parameters are calculated for every peptide on the basis of the contributions of its amino acid residues determining its degree of retention and its UV spectrum. The contribution values are experimentally found from chromatograms of the free amino acids obtained by multiwavelength photometric detection under the conditions of the peptide chromatography. Thirty peptides have been chromatographed for the evaluation of the proposed method, and the correlation coefficients between the calculated and the experimental retention volumes have been found to be 0.95.

[Degradation of bis(2-ethylhexyl)phthalate by microorganisms of water and sediments of the Selenga river and Baikal Lake under experimental conditions]. / Degradatsiia bis-(2-étilgeksil)ftalata mikroorganizmami vody i donnykh osadkov r. Selengi i oz. Baikal v usloviiakh model'nogo éksperimenta.

Degradation of bis-(2-ethylhexyl)phthalate (BEHP) by microbial associations of water and bottom sediments of the Selenga River and Lake Baikal and by pure cultures of microbial species belonging to various taxa isolated from the sediments under discussion has been studied. It has been shown that intense biological degradation occurs in both water and sediments. The degrees of conversion in experimental closed systems on minimal media are 46 and 24%, respectively. The most active of the organisms studied is a Micromironospora actinomycete. It degraded BEHP by 36% of its initial concentration. Spore-forming bacteria and microorganisms of the genus Pseudomonas were less active (17-23% and 7-11%).