RESUMO
OBJECTIVES: This study characterised 48 Klebsiella pneumoniae isolates from outpatients with urinary tract infection in the micro-region of Ribeirão Preto, located in southeastern Brazil. METHODS: The isolates were identified by conventional biochemical and phenotypic tests and were confirmed as K. pneumoniae using a MALDI-TOF VITEK® MS system. Antimicrobial susceptibility testing was performed by the disk diffusion method as recommended by the Clinical and Laboratory Standards Institute (CLSI) using 38 different antibiotic discs. Fifteen ß-lactamase and ten virulence genes were investigated by PCR. Clonal relationships among the isolates were determined by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) and multilocus sequence typing (MLST). RESULTS: Of the 48 isolates, 29 (60.4%) were classified as multidrug-resistant. A total of 46 ß-lactamase genes were found in 27 (56.3%) of the isolates, with blaKPC being the most prevalent distributed in 18 isolates (37.5%). Moreover, 73 virulence genes were found in 30 isolates (62.5%). ERIC-PCR results showed high genetic diversity among the isolates. Twelve different sequence types (STs) were found by MLST (ST14, ST17, ST101, ST200, ST334, ST433, ST437, ST442, ST449, ST502, ST1246 and ST2729), with ST2729 being described for the first time in this study. Seven STs were grouped in clonal complex 258 (CC258) frequently associated with various resistance and virulence genes. CONCLUSIONS: These results raise concern about epidemiological surveillance related to colonisation of patients discharged from hospitals in order to prevent both the occurrence and spread of resistant bacterial infections in the community.
Assuntos
Farmacorresistência Bacteriana Múltipla , Infecções por Klebsiella/microbiologia , Klebsiella pneumoniae/isolamento & purificação , Tipagem de Sequências Multilocus/métodos , Infecções Urinárias/microbiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Proteínas de Bactérias/metabolismo , Brasil , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Klebsiella pneumoniae/genética , Masculino , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Pacientes Ambulatoriais , Fatores de Virulência/genética , Adulto Jovem , beta-Lactamases/genéticaRESUMO
Enterobacter cloacae and E. aerogenes have been increasingly reported as important opportunistic pathogens. In this study, a high prevalence of multi-drug resistant isolates from Brazil, harboring several ß-lactamase encoding genes was found. Several virulence genes were observed in E. aerogenes, contrasting with the E. cloacae isolates which presented none.
Assuntos
Proteínas de Bactérias/metabolismo , Enterobacter aerogenes/isolamento & purificação , Enterobacter cloacae/isolamento & purificação , Infecções por Enterobacteriaceae/microbiologia , Fatores de Virulência/metabolismo , beta-Lactamases/metabolismo , Adulto , Idoso , Antibacterianos/farmacologia , Proteínas de Bactérias/genética , Brasil , Enterobacter aerogenes/classificação , Enterobacter aerogenes/enzimologia , Enterobacter aerogenes/genética , Enterobacter cloacae/classificação , Enterobacter cloacae/enzimologia , Enterobacter cloacae/genética , Feminino , Humanos , Masculino , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Filogenia , Virulência , Fatores de Virulência/genética , beta-Lactamases/genéticaRESUMO
ABSTRACT Enterobacter cloacae and E. aerogenes have been increasingly reported as important opportunistic pathogens. In this study, a high prevalence of multi-drug resistant isolates from Brazil, harboring several β-lactamase encoding genes was found. Several virulence genes were observed in E. aerogenes, contrasting with the E. cloacae isolates which presented none.
Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Proteínas de Bactérias/metabolismo , beta-Lactamases/metabolismo , Enterobacter cloacae/isolamento & purificação , Enterobacter aerogenes/isolamento & purificação , Fatores de Virulência/metabolismo , Infecções por Enterobacteriaceae/microbiologia , Filogenia , Proteínas de Bactérias/genética , Virulência , beta-Lactamases/genética , Brasil , Testes de Sensibilidade Microbiana , Enterobacter cloacae/classificação , Enterobacter cloacae/enzimologia , Enterobacter cloacae/genética , Enterobacter aerogenes/classificação , Enterobacter aerogenes/enzimologia , Enterobacter aerogenes/genética , Fatores de Virulência/genética , Pessoa de Meia-Idade , Antibacterianos/farmacologiaRESUMO
ABSTRACT Enterobacter cloacae and E. aerogenes have been increasingly reported as important opportunistic pathogens. In this study, a high prevalence of multi-drug resistant isolates from Brazil, harboring several -lactamase encoding genes was found. Several virulence genes were observed in E. aerogenes, contrasting with the E. cloacae isolates which presented none.
