RESUMO
BACKGROUND/PURPOSE: Apoptotic factors inducing or preventing cell death may intrinsically govern the behavior of some tumors. Survivin is a recently described member of the inhibitor of apoptosis protein (IAP) family, that is expressed in a cell cycle-dependent manner and is found in tumors of unfavorable histology. This study examines the presence of several apoptotic factors, including survivin, in neuroblastoma (NB) tumors. Clues to survivin's function in NB are provided by examining its association with behavior and cell dynamics in tumors and cell lines. METHODS: Expression of a panel of apoptosis factors were quantified in 15 NB and related tumors before chemotherapy and in 3 NB cell lines (NB7, NB10, and NB16). Survivin and other apoptotic factors, as well N-myc amplification in primary tumors was correlated with recurrent disease and outcome. Proliferation rate, apoptosis assays, cell cycle analysis, and drug- or immune-mediated cell death were assessed in cell lines and evaluated in the context of differential survivin and apoptosis gene expression. RESULTS: All 7 tumors that went on to recur expressed survivin, whereas expression was absent in all 8 tumors that went into remission. N-myc was amplified in 4 (57.1%) of the 7 recurrent tumors. Of the 8 tumors that were cured, Fas was expressed in 3 (38%), TRAIL-R1 in 6 (75%) and tumor necrosis factor (TNF)-R1 in 8 (100%), whereas these pro-apoptotic receptors were present in only 1 (14%), 1 (14%), and 4 (57%) of the 7 tumors that went on to recur, respectively. Of the 3 cell lines, NB10 expressed the least survivin, displayed the lowest proliferation index, and had the fewest number of cells in the G2/M (mitotic) phase of the cell cycle. Furthermore, NB10 also was most sensitive to TNF-related apoptosis-inducing ligand (TRAIL) or etoposide-induced cell death. CONCLUSIONS: In primary NB tumors, survivin expression was associated with tumors of high risk and unfavorable prognosis, whereas pro-apoptotic receptor expression was more abundant in tumors of favorable prognosis. In this small series, survivin expression appeared to be more predictive of recurrent disease than N-myc amplification. In cell lines, survivin expression was cell cycle dependent, and its expression was associated with greater proliferation rates and greater resistance to drug- or immune-mediated cell death. Survivin expression may become a useful prognostic marker in NB and could be a potential target for the treatment of this tumor. J Pediatr Surg 36:1785-1791.
Assuntos
Apoptose/genética , Proteínas Cromossômicas não Histona/antagonistas & inibidores , Proteínas Cromossômicas não Histona/biossíntese , Inibidores de Cisteína Proteinase/biossíntese , Neoplasias Renais/patologia , Proteínas Associadas aos Microtúbulos , Neuroblastoma/patologia , Proteínas Reguladoras de Apoptose , Biomarcadores Tumorais , Ciclo Celular/efeitos dos fármacos , Ciclo Celular/genética , Inibidores de Cisteína Proteinase/metabolismo , Etoposídeo/farmacologia , Expressão Gênica , Humanos , Proteínas Inibidoras de Apoptose , Neoplasias Renais/diagnóstico , Neoplasias Renais/metabolismo , Glicoproteínas de Membrana/farmacologia , Proteínas de Neoplasias , Neuroblastoma/diagnóstico , Neuroblastoma/metabolismo , RNA Mensageiro/metabolismo , Survivina , Ligante Indutor de Apoptose Relacionado a TNF , Células Tumorais Cultivadas , Fator de Necrose Tumoral alfa/farmacologiaRESUMO
OBJECTIVE: We examined the soluble CD23 (sCD23) molecules in sera and saliva from patients with Sjögren's syndrome. METHODS: The determination of sCD23 and other soluble molecules were made by the enzyme-linked immunosorbent assay. RESULTS: The amounts of sCD23 in the sera/saliva were significantly increased in the patients compared to the controls and the levels were significantly correlated with sialoectasis. CONCLUSION: The findings suggest that increased sCD23 molecules in saliva from patients with Sjögren's syndrome may reflect active sialoectasis.
Assuntos
Receptores de IgE/biossíntese , Saliva/química , Glândulas Salivares/metabolismo , Glândulas Salivares/patologia , Adulto , Feminino , Humanos , Imunoglobulinas/análise , Pessoa de Meia-Idade , Receptores de IgE/sangue , Saliva/imunologia , Síndrome de SjogrenRESUMO
The scanning laser tomograph was used to measure the optic disk topography in the left eye of five normal volunteers and five open angle glaucoma patients during the same day. We studied the reproducibility of 7 topographic parameters with the coefficient of variation (CV) and coefficient of reliability (CR) from topographic images of the scanning laser tomograph. The mean CV of the topographic parameters was 8.1 +/- 3.3% (range: 5.3-15.0%) and the mean CR of the topographic parameters was 91.8 +/- 7.6% (range: 78.6-98.4%) in normal eyes. In glaucoma eyes, the mean CV was 7.2 +/- 3.7% (range: 4.4-15.0%) and the mean CR was 84.8 +/- 7.5% (range: 73.4-95.3%), respectively. The results showed that the scanning laser tomograph has good reproducibility for clinical use in optic disk measurements.
