RESUMO
Aim: In Kampo medicine, there exists an important system of diagnosis called Fukushin, or abdominal diagnosis or palpation. By applying pressure to the abdomen of the patient, the physician can gain important information on the patient's physical state and use those indications to choose a suitable Kampo formulation. We have previously developed a Fukushin simulator, a teaching tool that reproduces the important abdominal patterns that doctors will encounter in clinical practice and that has received favourable feedback for students and practitioners. In order to make diagnosis and prescription easier, it is desirable to have matched formula-pattern pairings. The present study aims to develop such pairings. Methods: With the previously developed models as a foundation, in the present study the production team (two members) used materials such as urethane foam and silicone rubber to build an additional 13 standard abdominal pattern models matched to Kampo herbal formulas commonly used by practitioners in Japan. Subsequently, the evaluation team (the remaining 10 authors) investigated the viability of these models. Results: The evaluation team determined that abdominal pattern models matched to the following typical Kampo formulas were created successfully: Dai-saiko-To (), Dai-joki-To (), Shigyaku-San (), Saiko-ka-ryukotsu-borei-To (), Keishi-bukuryo-Gan (), Hachimi-jio-Gan (), Hange-shashin-To (), Sho-saiko-To (), Hochu-ekki-To (), Sho-kenchu-To (), Toki-shakuyaku-San (), Ninjin-To (), and Dai-kenchu-To (). Conclusion: We suggest that these new formula-pattern models can make an important contribution to the standardization of abdominal diagnosis and prescription and to Kampo education.
RESUMO
Seven novel norcycloartane glycosides, maryloside A-G (1-7), were isolated from the leaves of Cymbidium Great Flower 'Marylaurencin', along with a known norcycloartane glycoside, cymbidoside (8). These structures were determined on the basis of mainly NMR experiments as well as chemical degradation and X-ray crystallographic analysis. The isolated compounds (1-6 and 8) were evaluated for the inhibitory activity on lipopolysaccharide (LPS) and interferon-γ (IFN-γ)-stimulated nitric oxide (NO) production in RAW 264.7 cells. Consequently, 1 and 3 exhibited moderate activity.
Assuntos
Flores/química , Glicosídeos/química , Orchidaceae/química , Folhas de Planta/química , Sobrevivência Celular , Flores/metabolismo , Glicosídeos/isolamento & purificação , Espectroscopia de Ressonância Magnética , Modelos Moleculares , Estrutura Molecular , Óxido Nítrico/biossíntese , Orchidaceae/metabolismo , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Folhas de Planta/metabolismoRESUMO
The roots and stolons of some Glycyrrhiza species are used worldwide for traditional folk medicines and commercial pharmaceuticals. Phenolic constituents such as flavonoids and coumarins are medicinal and vary according to species. Therefore, species identification is important for quality analysis. In order to identify Glycyrrhiza species by chemical fingerprinting, methanol extracts of the root bark of Glycyrrhiza uralensis Fischer and G. glabra Linn6 were analyzed using EI-MS. Differences in kinds and quantity of components are reflected in complex EI-MS data and determining characteristic peaks for each species is straightforward.. The chaiacteristic peaks were determined statistically by volcano plot, a multivariate analysis method. EI-MS data of G. uralensis and G. glabra showed differential patterns, and the notable peaks in each pattern were identified. Peaks at m/z 153 and 221 are signature peaks of G. uralensis, and at 11/z 173, 309, and 324 are those of G. glabra. In conclusion, we found species-specific patterns by EI-MS that distinguish G. uralensis and G. glabra. This method based on chemical constituent patterns can be applied to identify other Glycyrrhiza species and similar natural products.
Assuntos
Glycyrrhiza/química , Cromatografia Líquida de Alta Pressão , Cromatografia por Troca Iônica , Metabolômica , Recursos Naturais , Mapeamento de Peptídeos , Extratos Vegetais/química , Raízes de Plantas/química , Especificidade da Espécie , Espectrometria de Massas por Ionização por ElectrosprayRESUMO
In order to identify the country of growth of Sophora flavescens by chemical fingerprinting, extracts of plants grown in China and Japan were analyzed using direct analysis in real time mass spectrometry (DART)-MS. The peaks characteristic of each country of growth were statistically analyzed using a volcano plot to summarize the relationship between the p-values of a statistical test and the magnitude of the difference in the peak intensities of the samples in the groups. Peaks with ap value < 0.05 in the t-test and a ≥ 2 absolute difference were defined as characteristic. Peaks characteristic of Chinese S. flavescens were found at m/z 439 and 440. In contrast, peaks characteristic of Japanese S. flavescens were found at m/z 313, 423, 437 and 441. The intensity of the selected peaks was similar in Japanese samples, whereas the m/z 439 peak had a significantly higher intensity than the other peaks in Chinese samples. Therefore, differences in selected peak patterns may allow identification of the country of growth of S. flavescens.
Assuntos
Espectrometria de Massas/métodos , Sophora/química , China , JapãoRESUMO
BACKGROUND: Castanea mollissima Blume (Chinese chestnut), as a food product is known for its various nutrients and functional values to the human health. The present study was carried out to analyze the anti-diabetic complications and anti-cancer activities of the bioactive compounds present in C. mollissima. METHODS: The kernels (CK), shells (CS) and involucres (CI) parts of C. Blume were extracted with 90% alcohol. The water suspension of these dried alcohol extracts were extracted using EtOAc and n-BuOH successively. The n-BuOH fraction of CI (CI-B) was isolated by silica gel column, Sephadex LH 20 column and preparative HPLC. The isolated compounds were identified by 1H-NMR, 13C-NMR, HMBC, HMQC and ESI-Q-TOF MS, All the fractions and compounds isolated were evaluated on human recombinant aldose reductase (HR-AR) assay, advanced glycation end products (AGEs) formation assay and human COLO 320 DM colon cancer cells inhibitory assay. RESULTS: CI-B was found to show a significant inhibitory effect in above biological screenings. Six flavonoids and three polyphenolic acids were obtained from CI-B. They were identified as kaempferol (1), kaempferol-3-O-[6''-O-(E)-p-coumaroyl]-ß-D-glucopyranoside (2), kaempferol-3-O-[6''-O-(E)-p-coumaroyl]-ß-D-galactopyranoside (3), kaempferol-3-O-[2''-O-(E)-p-coumaroyl]-ß-D-glucopyranoside (4), kaempferol-3-O-[2", 6"-di-O-(E)-p-coumaroyl]-ß-D-glucopyranoside (5) and kaempferol-3-O-[2", 6"-di-O-(E)-p-coumaroyl]-ß-D-galactopyranoside (6), casuariin (7), casuarinin (8) and castalagin (9). Compounds 2-9 were found to show higher activity than quercetin (positive control) in the AR assay. Compounds 3-6, 8, and 9 showed stronger inhibitory effects than amino guanidine (positive control) on AGEs production. Compounds 4-6, 7, and 8 showed much higher cytotoxic activity than 5-fluorouracil (positive control) against the human COLO 320 DM colon cancer cells. CONCLUSIONS: Our results suggest that flavonoids and polyphenolic acids possesses anti-diabetes complications and anti-cancer properties, and they were presumed to be the bioactive components of Castanea mollissima Blume.
Assuntos
Antineoplásicos Fitogênicos/química , Fagaceae/química , Hipoglicemiantes/química , Extratos Vegetais/química , Aldeído Redutase/antagonistas & inibidores , Aldeído Redutase/metabolismo , Antineoplásicos Fitogênicos/farmacologia , Cromatografia Líquida de Alta Pressão , Complicações do Diabetes/tratamento farmacológico , Complicações do Diabetes/enzimologia , Inibidores Enzimáticos/química , Inibidores Enzimáticos/farmacologia , Flavonoides/química , Flavonoides/farmacologia , Fluoruracila/química , Fluoruracila/farmacologia , Humanos , Hipoglicemiantes/farmacologia , Estrutura Molecular , Neoplasias/tratamento farmacológico , Neoplasias/enzimologia , Extratos Vegetais/farmacologiaRESUMO
In order to identify Glycyrrhiza species by chemical fingerprinting, the bark of the roots and stolons of Glycyrrhiza uralensis Fischer and G. glabra Linné were analyzed using DART (Direct Analysis in Real Time)-MS. The characteristic peaks of each species were determined statistically by volcano plot. This summarizes the relationship between the p-values of a statistical test and the magnitude of the difference in values of the samples in the groups. In this experiment, peaks that had a p value <0.05 in the t test and Z2 absolute difference were defined as characteristic. As a result, characteristic peaks of G. uralensis were found at m/z 299, 315, 341, and 369. In contrast, characteristic peaks of G. glabra were found at m/z 323, 325, 337, 339, and 391. In conclusion, we found several characteristic peaks to distinguish G. uralensis and G. glabra by DART-MS using volcano plot. This method can be applied to identify the Glycyrrhiza species.
Assuntos
Glycyrrhiza uralensis/química , Glycyrrhiza uralensis/classificação , Espectrometria de Massas , Casca de Planta/química , Especificidade da EspécieRESUMO
The research in our laboratory focuses on the isolation of saponins from cactus. In this study, we report five new triterpenoid saponins, dumortierinoside A methyl ester (1), pachanoside I1 (2), pachanoside D1 (3), gummososide A (4), and gummososide A methyl ester (5). Compounds 1-3 isolated from Isolatocereus dumortieri Backbg., and compounds 4 and 5 were isolated from Stenocereus alamosensis A. C. Gibson & K. E. Horak. Compound 2 possessed a new pachanane-type triterpene skeleton, pachanol I, in its aglycon. The aglycon of 3 was pachanol D, while those of 4 and 5 were both gummosogenin, which we have previously reported, but this is the first report of pachanol D and gummosogenin in their aglycon forms. Additionally, we evaluated the anti-type I allergy activity of the saponins with RBL-2H3 (Rat basophilic leukemia) cells by measuring the ß-hexosaminidase release inhibitory activity. As a result of these studies, gummososide A methyl ester (5) was found to show activity (IC(50)=99.5 µM) and thurberoside A exhibited mild activity (IC(50)=166.9 µM).
Assuntos
Antialérgicos/química , Cactaceae/química , Saponinas/química , Triterpenos/química , Animais , Antialérgicos/isolamento & purificação , Antialérgicos/farmacologia , Linhagem Celular Tumoral , Modelos Moleculares , Estrutura Molecular , Ratos , Saponinas/isolamento & purificação , Saponinas/farmacologia , Relação Estrutura-Atividade , Triterpenos/isolamento & purificação , Triterpenos/farmacologia , beta-N-Acetil-Hexosaminidases/metabolismoRESUMO
This study presents the application of the NMR-based analyses, DOSY and ROSY, to the chalcones, xanthoangelol (1) and 4-hydroxyderricin (2) from Angelica keiskei. We investigated whether virtual separation and structural information from each compound can be obtained. DOSY displays spectra of (1) and (2) in one dimension and diffusion spectra in the other. And the 1H slice spectra were analyzed in detail by comparison with authentic samples previously isolated from the same material. The resulting ROSY spectrum clearly showed two distinct peaks in the 1H T1 dimension. Each slice of the ROSY spectrum along the 13C dimension contains over-lapped signals, which are difficult to assign at this time.
Assuntos
Angelica/química , Chalconas/análise , Espectroscopia de Ressonância Magnética/métodos , Misturas Complexas/análiseRESUMO
We demonstrate that NMR-based metabolomics can be used to identify the country of growth (Japan or China) of Sophora flavescens plants. Principle Component Analysis (PCA) conducted on extracts of S. flavescens grown in China provided data distinct from that of extracts of plants grown in Japan. Loading plot analysis showed signals characteristic of Japanese S. flavescens. NMR analyses showed these signals to be due to kurarinol (1) and kushenol H (2). These compounds were confirmed by HPLC analysis to be distinctive markers for Japanese S. flavescens.
Assuntos
Sophora/metabolismo , China , Japão , Espectroscopia de Ressonância Magnética , Metaboloma , Análise de Componente Principal , Sophora/classificaçãoRESUMO
CONTEXT: Prevalence of diabetes mellitus type 2 (DM-II) is increasing in Japan. Brown alga Ecklonia kurome Okamura (Laminariaceae) (kurome in Japanese) is rich in phlorotannins, a kind of polyphenol. Phlorotannins have been reported to possess various bioactivities; however, few studies have reported its effect on DM-II. OBJECTIVE: The present study was conducted to investigate the antidiabetic effect of polyphenols from E. kurome (KPP) on KK-A(y) mice, the animal model for human DM-II. MATERIALS AND METHODS: Inhibitory activities of KPP against α-amylase and α-glucosidase in vitro, and effects on oral carbohydrate tolerance test in vivo were investigated. KK-A(y) mice were fed with 0.1% KPP containing water for 5 weeks. A glucose tolerance test was conducted at week 4 of the 5-week period. At the end of experiment, blood biochemical parameters, including blood glucose, insulin, glycoalbumin, and fructosamine were determined. Furthermore, the kidneys and pancreatic islets were histologically examined. RESULTS: KPP showed inhibitory activities on carbohydrate-hydrolyzing enzymes and decreased postprandial blood glucose levels. The body weight gain and blood glucose levels in the KPP group were lower than the control group during the experimental period. KPP improved glucose tolerance and decreased the fasting blood glucose and insulin levels, fructosamine and glycoalbumin levels compared with the control group. Furthermore, KPP contracted the pancreatic islet size and decreased renal mesangial matrix in KK-A(y) mice. DISCUSSION AND CONCLUSION: These results suggest that KPP is effective against DM-II and might provide a source of therapeutic agents for DM-II.
Assuntos
Diabetes Mellitus Tipo 2/tratamento farmacológico , Hipoglicemiantes/farmacologia , Phaeophyceae/química , Polifenóis/farmacologia , Animais , Glicemia/efeitos dos fármacos , Diabetes Mellitus Experimental/tratamento farmacológico , Diabetes Mellitus Experimental/fisiopatologia , Diabetes Mellitus Tipo 2/fisiopatologia , Teste de Tolerância a Glucose , Inibidores de Glicosídeo Hidrolases , Hipoglicemiantes/isolamento & purificação , Insulina/metabolismo , Ilhotas Pancreáticas/metabolismo , Rim/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos ICR , Polifenóis/isolamento & purificação , Período Pós-Prandial , alfa-Amilases/antagonistas & inibidoresRESUMO
Recently, NMR-based metabolomic analysis has been used to acquire information based on differentiation among biological samples. In the present study, we examined whether multivariate analysis was able to be applied to natural products and/or material field. Each extraction of 24 leaf samples, divided into six locations from the tip of the stem in each of four strains, was analyzed by pattern recognition methods, known as Principal Component Analysis (PCA) and Soft Independent Modeling of Class Analogy (SIMCA). Twenty-four extracts from mulberry leaf showed independent spectra by 1H NMR. The separation of leaf extraction data due to the difference at six locations was achieved in the PCA score plot as correlation PC1 (86.1%) and PC3 (4.6%) and showed two loading plots, suggesting classification by leaf position as an independent variable in the loading plot. Moreover, the difference among six locations clarified the seven highest discrimination powers by the SIMCA method. Meanwhile, the PCA score plot obtained classification by the variety of mulberry strains with three loading plots, but the SIMCA method did not give a peak by classification.
Assuntos
Metaboloma , Morus/química , Espectroscopia de Ressonância Magnética , Metabolômica , Morus/classificação , Morus/metabolismo , Análise Multivariada , Extratos Vegetais/química , Extratos Vegetais/classificação , Folhas de Planta/química , Folhas de Planta/classificação , Folhas de Planta/metabolismo , Análise de Componente PrincipalRESUMO
Linear-type furocoumarins, 5-[(2"E,6"R)-6"-hydroxy-3",7"-dimethylocta-2",7"-dienyloxy]psoralen and 5-[(2"E,6"S)-6"-hydroxy-3",7"-dimethylocta-2",7"-dienyloxy]psoralen (1) were first isolated from whole plants of Seseli hartvigii together with one new natural product 6-(3'-methyl-2'-oxo-3'-butenyl)-7-methoxycoumarin (2), and four known compounds (tamarin, bergaptol, notoptol, and a mixture of beta-sitosterol and stigmasterol). The structure of 1 was elucidated by extensive spectroscopic analysis and chemical conversion. The modified Mosher's method and HPLC were applied to determine its stereochemistry. Both R- and S-configurations exist in 1; after modification by Mosher's reagent, they were effectively separated, and their ratio was deduced to be 59% and 41%, respectively.
Assuntos
Apiaceae/química , Cumarínicos/química , Cromatografia Líquida de Alta Pressão , Espectroscopia de Ressonância Magnética , Estrutura Molecular , EstereoisomerismoRESUMO
Urtica cannabina L. (Urticaceae) is a perennial herb that grows in Xinjiang Uighur Autonomous Region (northwest China). Two megastigmanes (1, 2) and five flavonoid glycosides (3-7) were isolated from its fruit. Compound 1 was determined by spectroscopic analysis to be (+)-blumenol A, and its absolute stereochemistry was determined in detail using chemical conversion and a modification of Mosher's method. Other compounds were identified as (+)-dehydrovomifoliol (2), isovitexin (3), isoquercitrin (4), astragalin (5), afzelin (6), and quercitrin (7) using spectroscopic (NMR, HMBC, MS) and physical methods (melting point and optical rotation). Compounds 1-7 were isolated from this plant for the first time, while this is the first report of megastigmanes in the Urticaceae family. The chemotaxonomic significance of the isolation of these megastigmanes and flavonoid glycosides from Urtica species is discussed.
Assuntos
Medicamentos de Ervas Chinesas/isolamento & purificação , Medicamentos de Ervas Chinesas/uso terapêutico , Medicina Tradicional Chinesa , Urticaceae , China , Medicamentos de Ervas Chinesas/química , FrutasRESUMO
DART (Direct Analysis in Real Time)-MS is a novel mass spectrometric ion source, and allows the analysis of most compounds at ambient pressure and ground potential by producing [M+H]+ molecular ion species. Using this method, we examined the compounds characteristic of several kinds of licorices. For the analysis of Glycyrrhiza inflata Batalin, the peak at m/z 339 originates mainly from [M+H]+ of licochalcone A (LA), a species-specific compound. This peak was hardly detected in G. glabra Linné and G. uralensis Fischer. These results indicate that G. inflata can be differentiated from the other two species by detection of LA peaks using DART-MS analysis.
Assuntos
Glycyrrhiza/classificação , Espectrometria de Massas/métodos , Estrutura Molecular , Raízes de Plantas/química , Especificidade da EspécieRESUMO
We compared the inhibitory effects on melanogenesis of six plant parts (leaves, stems, roots, whole fruits, calyxes, and fruits without calyxes) of Cucumis sativus. MeOH extracts of leaves and stems inhibited melanin production in B16 cells. These extracts did not affect the activity of mushroom tyrosinase or crude enzyme lysate from B16 cells. However, the extracts decreased tyrosinase expression at the protein level. These results suggest that the depigmenting mechanism of extracts from leaves and stems of C. SATIVUS involves the expression of tyrosinase. Of eight compounds isolated from the leaves, lutein ( 1) (IC (50) = 170.7 microM) and (+)-(1 R,2 S,5 R,6 S)-2,6-di-(4'-hydroxyphenyl)-3,7-dioxabicyclo[3.3.0]octane ( 2) (IC (50) = 270.8 microM) were found to suppress melanogenesis. Whereas 1 was found to markedly decrease the expression levels of tyrosinase, 2 only weakly reduced tyrosinase expression. This suggests that 1 is an active component in the leaves of C. sativus and is a potentially useful skin-whitening agent.
Assuntos
Cucumis sativus , Melaninas/biossíntese , Melanoma Experimental/metabolismo , Monofenol Mono-Oxigenase/metabolismo , Extratos Vegetais/farmacologia , Animais , Antioxidantes/farmacologia , Cucumis sativus/química , Hiperpigmentação/terapia , Camundongos , Monofenol Mono-Oxigenase/efeitos dos fármacos , Fitoterapia , Folhas de Planta , Caules de Planta , Pironas/farmacologia , Células Tumorais CultivadasRESUMO
A new simple coumarin glycoside, named praeroside VI (1), along with six known coumarins, were isolated from an aqueous extract of Bai-Hua Qianhu, the root of Peucedanum praeruptorum DUNN. (Umbelliferae). Spectroscopic analysis and chemical studies were carried out to investigate the structure of the new coumarin, which was concluded to be 1. Additionally, two simple glycosidic coumarins and four simple nonglycosidic coumarins were identified as apiosylskimmin (2), hymexelsin (3), umbelliferone (4), scopoletin (5), isofraxidin (6) and 8-carboxy-7-hydroxy coumarin (7), respectively. This is the first reported identification of compound 7 as a constituent of plant materials.
Assuntos
Apiaceae/química , Cumarínicos/química , Sequência de Carboidratos , Hidrólise , Espectroscopia de Ressonância Magnética , Dados de Sequência Molecular , Raízes de Plantas/química , Espectrometria de Massas por Ionização por Electrospray , Espectrometria de Massas de Bombardeamento Rápido de Átomos , Espectrofotometria UltravioletaRESUMO
Two new megastigmane, cucumegastigmanes I (1) and II (2), together with a known megastigmane, (+)-dehydrovomifoliol (3), and five other known compounds were isolated from the leaves of Cucumis sativus. The structures of the new compounds were elucidated from spectroscopic analysis and their absolute stereochemistries were determined in detail using the chemical conversion and a modified Mosher's method.
Assuntos
Cucumis sativus/química , Norisoprenoides/isolamento & purificação , Folhas de Planta/química , Norisoprenoides/química , Análise Espectral/métodosRESUMO
Cellular damage induced by chronic inflammation is a well known cause of colon carcinogenesis. Cyclooxygenase-2 (COX-2), the enzyme that converts arachidonic acid to prostanoids, is known to play an important role in inflammation. Herbal flavonoid isoliquiritigenin (ILTG) has previously been reported to be a strong suppresser of the COX-2 pathway as well as an inducer of apoptosis. Here we report that the susceptibility to apoptosis by ILTG is dependent on the level of COX-2 in mouse colon adenocarcinoma Colon 26, which spontaneously expresses COX-2. This dependency was observed to be enhanced by blockage of the lipoxigenases (LOXs)-mediated metabolic pathway and attenuated by addition of a number of prostaglandins and thromboxanes. Taken together, these findings indicate that ILTG-induced apoptosis is negatively regulated by the COX-2 expression level.
Assuntos
Adenocarcinoma/tratamento farmacológico , Apoptose/efeitos dos fármacos , Chalcona/análogos & derivados , Neoplasias do Colo/tratamento farmacológico , Inibidores de Ciclo-Oxigenase 2/uso terapêutico , Ciclo-Oxigenase 2/fisiologia , Adenocarcinoma/enzimologia , Adenocarcinoma/patologia , Animais , Caspase 3 , Caspases/metabolismo , Chalcona/uso terapêutico , Chalconas , Neoplasias do Colo/enzimologia , Neoplasias do Colo/patologia , Camundongos , Plantas/química , Poli(ADP-Ribose) Polimerases/metabolismo , Prostaglandinas/metabolismo , Tromboxanos/metabolismo , Células Tumorais CultivadasRESUMO
Artepillin C, a prenylated phenylpropanoid found specifically in Brazilian propolis, has been shown to be a bioavailable antioxidant. In this study, artepillin C was tested for colon cancer-preventing activity using azoxymethane-challenged ddY mice. Oral doses of 80 and 160 mg/kg body weight of propolis or 10mg/kg of artepillin C (equi-amounts to 160 mg propolis) reduced significantly the frequency of colonic aberrant crypt foci (ACF) by 39.2, 43.7 and 43.4%, respectively. In liver of the mice, glutathione S-transferase and NADPH:quinone reductase activity increased with the doses of propolis or artepillin C, and an antioxidant-responsive element (ARE) was found to be activated for binding DNA. Artepillin C is considered to suppress the formation of colonic ACF through the activation of ARE and induction of phase II enzymes in liver.
Assuntos
Antineoplásicos/farmacologia , Antioxidantes/farmacologia , Neoplasias do Colo/prevenção & controle , Mucosa Intestinal/efeitos dos fármacos , Fenilpropionatos/farmacologia , Lesões Pré-Cancerosas/prevenção & controle , Animais , Antineoplásicos/administração & dosagem , Azoximetano , Carcinógenos , Neoplasias do Colo/induzido quimicamente , Dieta , Relação Dose-Resposta a Droga , Glutationa Transferase/genética , Glutationa Transferase/metabolismo , Mucosa Intestinal/patologia , Fígado/efeitos dos fármacos , Fígado/enzimologia , Masculino , Camundongos , NAD(P)H Desidrogenase (Quinona)/genética , NAD(P)H Desidrogenase (Quinona)/metabolismo , Fenilpropionatos/administração & dosagem , Lesões Pré-Cancerosas/induzido quimicamente , Própole/administração & dosagem , Própole/farmacologia , Elementos de Resposta/efeitos dos fármacos , Regulação para CimaRESUMO
Daphne genkwa (Thymelaeaceae) has been used as a folk medicine in China. We investigated the effects of D. genkwa and Jyu-So-To on various pharmacologic models in mice including the azoxymethane (AOM)-induced colonic aberrant crypt focus formation assay, ornithine decarboxylase (ODC) activity assay, and two types of mouse ear swelling model. Administration of 236.3 ppm of Jyu-So-To in drinking water significantly suppressed AOM-induced colonic aberrant crypt focus formation (p < 0.05), with an inhibitory ratio of 46.7%. The effects of several extracts with organic solvents of D. genkwa on murine epidermal ODC activity were examined. In particular, the inhibitory ratio of the n-hexane extract was 30.8%. In the 12-O-Tetradecanoylphorbol-13-acetate (TPA)-induced ear edema model in mice, the methanol extract resulted in 56.3% inhibition compared with the control. On the other hand, there are two peaks of responses at 1 h (immediate-phase reaction; IPR) and 24 h (late-phase reaction) in biphasic cutaneous reactions, which are enhanced in the dinitrofluorobenzene model (DNFB). The water extract of D. genkwa clearly inhibited the IPR ear swelling. These results suggest that D. genkwa and Jyu-So-To should be a promising source of antitumor, antiinflammatory, and antiallergy agents.