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1.
ACS Appl Mater Interfaces ; 10(39): 33545-33555, 2018 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-30192508

RESUMO

Antibiotic resistance in bacterial cells has motivated the scientific community to design new and efficient (bio)materials with targeted bacteriostatic and/or bactericide properties. In this work, a series of polyelectrolyte multilayer films differing in terms of polycation-polyanion combinations are constructed according to the layer-by-layer deposition method. Their capacities to host T4 and φx174 phage particles and maintain their infectivity and bacteriolytic activity are thoroughly examined. It is found that the macroscopic physicochemical properties of the films, which includes film thickness, swelling ratio, or mechanical stiffness (as derived by atomic force microscopy and spectroscopy measurements), do not predominantly control the selectivity of the films for hosting infective phages. Instead, it is evidenced that the intimate electrostatic interactions locally operational between the loaded phages and the polycationic and polyanionic PEM components may lead to phage activity reduction and preservation/enhancement, respectively. It is argued that the underlying mechanism involves the screening of the phage capsid receptors (operational in cell recognition/infection processes) because of the formation of either polymer-phage hetero-assemblies or polymer coating surrounding the bioactive phage surface.


Assuntos
Bacteriófagos/patogenicidade , Materiais Biocompatíveis/química , Bioensaio/métodos , Polímeros/química , Difusão Dinâmica da Luz , Microscopia de Força Atômica , Polieletrólitos
2.
Biomed Mater Eng ; 28(s1): S95-S100, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28372283

RESUMO

BACKGROUND: Human tissue derived natural extracellular matrix (ECM) has great potential in tissue engineering. OBJECTIVE: We sought to isolate extracellular matrix derived from human umbilical cord and test its potential in tissue engineering. METHODS: An enzymatic method was applied to isolate and solubilized complete human umbilical cord derived matrix (hUCM). The obtained solution was analyzed for growth factors, collagen and residual DNA contents, then used to coat 2D and 3D surfaces for cell culture application. RESULTS: The hUCM was successfully isolated with trypsin digestion to acquire a solution containing various growth factors and collagen but no residual DNA. This hUCM solution can form a coating on 2D and 3D substrates suitable cell culture. CONCLUSION: We developed a new matrix derived from human source that can be further used in tissue engineering.


Assuntos
Matriz Extracelular/química , Células Endoteliais da Veia Umbilical Humana/citologia , Células-Tronco Mesenquimais/citologia , Alicerces Teciduais/química , Cordão Umbilical/química , Materiais Biocompatíveis/química , Adesão Celular , Técnicas de Cultura de Células/métodos , Proliferação de Células , Células Cultivadas , Colágeno/análise , DNA/análise , Humanos , Peptídeos e Proteínas de Sinalização Intercelular/análise , Engenharia Tecidual/métodos
3.
ACS Omega ; 2(3): 918-929, 2017 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-30023620

RESUMO

Delivery of growth factors is an indispensable part of tissue engineering. Here, we describe a detachable membrane-based release system composed of extracellular matrix components that can be attached to hydrogels to achieve directional release of bioactive molecules. This way, the release of cytokines/growth factors can be started at a desired point of tissue maturation or directly in vivo. As a model, we develop thin films of an interpenetrating network of double-cross-linked gelatin and hyaluronic acid derivatives. The use of the auxiliary release system with vascular endothelial growth factor results in extensive sprouting by encapsulated vascular endothelial cells. The presence of the release system with interleukin-4 results in clustering of encapsulated macrophages with a significant decrease in M1 macrophages (proinflammatory). This system can be used in conjunction with three-dimensional structures as an auxiliary system to control artificial tissue maturation and growth.

4.
Biomacromolecules ; 17(6): 2189-98, 2016 06 13.
Artigo em Inglês | MEDLINE | ID: mdl-27183396

RESUMO

This study aims to design an optimal polyelectrolyte multilayer film of poly-l-lysine (PLL) and hyaluronic acid (HA) as an anti-inflammatory cytokine release system in order to decrease the implant failure due to any immune reactions. The chemical modification of the HA with aldehyde moieties allows self-cross-linking of the film and an improvement in the mechanical properties of the film. The cross-linking of the film and the release of immunomodulatory cytokine (IL-4) stimulate the differentiation of primary human monocytes seeded on the films into pro-healing macrophages phenotype. This induces the production of anti-inflammatory cytokines (IL1-RA and CCL18) and the decrease of pro-inflammatory cytokines secreted (IL-12, TNF-α, and IL-1ß). Moreover, we demonstrate that cross-linking PLL/HA film using HA-aldehyde is already effective by itself to limit inflammatory processes. Finally, this functionalized self-cross-linked PLL/HA-aldehyde films constitutes an innovative and efficient candidate for immunomodulation of any kind of implants of various architecture and properties.


Assuntos
Reagentes de Ligações Cruzadas/química , Citocinas/administração & dosagem , Ácido Hialurônico/química , Imunomodulação/efeitos dos fármacos , Inflamação/tratamento farmacológico , Polieletrólitos/química , Adesão Celular/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Células Cultivadas , Citocinas/química , Humanos , Inflamação/imunologia , Macrófagos/citologia , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Monócitos/citologia , Monócitos/efeitos dos fármacos , Monócitos/metabolismo , Propriedades de Superfície
5.
Nanoscale ; 8(9): 5268-79, 2016 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-26879405

RESUMO

DDB2, known for its role in DNA repair, was recently shown to reduce mammary tumor invasiveness by inducing the transcription of IκBα, an inhibitor of NF-κB activity. Since cellular adhesion is a key event during the epithelial to mesenchymal transition (EMT) leading to the invasive capacities of breast tumor cells, the aim of this study was to investigate the role of DDB2 in this process. Thus, using low and high DDB2-expressing MDA-MB231 and MCF7 cells, respectively, in which DDB2 expression was modulated experimentally, we showed that DDB2 overexpression was associated with a decrease of adhesion abilities on glass and plastic areas of breast cancer cells. Then, we investigated cell nanomechanical properties by atomic force microscopy (AFM). Our results revealed significant changes in the Young's Modulus value and the adhesion force in MDA-MB231 and MCF7 cells, whether DDB2 was expressed or not. The cell stiffness decrease observed in MDA-MB231 and MCF7 expressing DDB2 was correlated with a loss of the cortical actin-cytoskeleton staining. To understand how DDB2 regulates these processes, an adhesion-related gene PCR-Array was performed. Several adhesion-related genes were differentially expressed according to DDB2 expression, indicating that important changes are occurring at the molecular level. Thus, this work demonstrates that AFM technology is an important tool to follow cellular changes during tumorigenesis. Moreover, our data revealed that DDB2 is involved in early events occurring during metastatic progression of breast cancer cells and will contribute to define this protein as a new marker of metastatic progression in this type of cancer.


Assuntos
Neoplasias da Mama , Proteínas de Ligação a DNA/biossíntese , Módulo de Elasticidade , Regulação Neoplásica da Expressão Gênica , Proteínas de Neoplasias/biossíntese , Neoplasias da Mama/química , Neoplasias da Mama/metabolismo , Neoplasias da Mama/ultraestrutura , Adesão Celular , Feminino , Humanos , Células MCF-7 , Microscopia de Força Atômica , Metástase Neoplásica
6.
Biochim Biophys Acta ; 1858(1): 75-84, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26525662

RESUMO

Against the increase of bacterial resistance to traditional antibiotics, antimicrobial peptides (AMP) are considered as promising alternatives. Bacterial biofilms are more resistant to antibiotics that their planktonic counterpart. The purpose of this study was to investigate the action of an AMP against a nascent bacterial biofilm. The activity of dermaseptin S4 derivative S4(1-16)M4Ka against 6 h-old Pseudomonas fluorescens biofilms was assessed by using a combination of Attenuated Total Reflectance-Fourier Transform InfraRed (ATR-FTIR) spectroscopy in situ and in real time, fluorescence microscopy using the Baclight™ kit, and Atomic Force Microscopy (AFM, imaging and force spectroscopy). After exposure to the peptide at three concentrations, different dramatic and fast changes over time were observed in the ATR-FTIR fingerprints reflecting a concentration-dependent action of the AMP. The ATR-FTIR spectra revealed major biochemical and physiological changes, adsorption/accumulation of the AMP on the bacteria, loss of membrane lipids, bacterial detachment, bacterial regrowth, or inhibition of biofilm growth. AFM allowed estimating at the nanoscale the effect of the AMP on the nanomechanical properties of the sessile bacteria. The bacterial membrane elasticity data measured by force spectroscopy were consistent with ATR-FTIR spectra, and they allowed suggesting a mechanism of action of this AMP on sessile P. fluorescens. The combination of these three techniques is a powerful tool for in situ and in real time monitoring the activity of AMPs against bacteria in a biofilm.


Assuntos
Proteínas de Anfíbios/farmacologia , Antibacterianos/farmacologia , Peptídeos Catiônicos Antimicrobianos/farmacologia , Biofilmes/efeitos dos fármacos , Pseudomonas fluorescens/efeitos dos fármacos , Proteínas de Anfíbios/síntese química , Antibacterianos/síntese química , Peptídeos Catiônicos Antimicrobianos/síntese química , Aderência Bacteriana/efeitos dos fármacos , Biofilmes/crescimento & desenvolvimento , Membrana Celular/química , Membrana Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Módulo de Elasticidade/efeitos dos fármacos , Lipídeos de Membrana/química , Testes de Sensibilidade Microbiana , Microscopia de Força Atômica , Microscopia de Fluorescência , Pseudomonas fluorescens/química , Pseudomonas fluorescens/crescimento & desenvolvimento , Pseudomonas fluorescens/ultraestrutura , Espectroscopia de Infravermelho com Transformada de Fourier
7.
Biomater Sci ; 3(9): 1302-11, 2015 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26261857

RESUMO

Mammalian cell culture is the starting point in many research studies focusing on biomedical applications. However, researchers have little control over the standardized cell microenvironment parameters. Here a modular ECM-mimicking surface coating for cell culture environment is designed. This substrate is a new and versatile thin film obtained by spin-coating of concentrated gelatin crosslinked by transglutaminase. It can be modified with respect to the biochemical and biophysical needs of the final cell destination, i.e. it delivers loaded multi-growth factors and serum components and allows for cell culture in a serum-free culture medium. Also, a well-known cell behavior modulator, the substrate stiffness, is controlled exogenously by addition of nanoparticles. In addition to growth factors, antimicrobial agents such as natural peptides are added to the substrate for limiting the repeated addition of antimicrobial agents to the culture medium and to prevent the increase of resistant bacterial strains in the culture environment. Finally, this substrate contains simultaneously ECM components, growth factors, stiffening elements and antimicrobial agents. It provides a favorable microenvironment and sterile conditions. It is a free-of-maintenance system, as cells will grow without addition of serum or antimicrobial cocktails. This low cost and easy-to-use substrate could emerge as a new standard for cell culture.


Assuntos
Anti-Infecciosos/química , Técnicas de Cultura de Células/métodos , Meios de Cultura/química , Matriz Extracelular/química , Soro/química , Animais , Microambiente Celular , Matriz Extracelular/metabolismo , Matriz Extracelular/fisiologia , Humanos
8.
J Mater Chem B ; 3(9): 1801-1812, 2015 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-32262253

RESUMO

The Bell-Evans theory relative to rupture forces between non-covalently interacting molecules predicts that the rupture force increases linearly with the logarithm of the force loading rate. Here we investigate by force spectroscopy performed with an atomic force microscope (AFM) the rupture forces between surfaces covered by ß-cyclodextrin (ß-CD) molecules and AFM tips coated with adamantane (AD) groups. The ß-CD molecules are either deposited through a self-assembled monolayer (SAM) or grafted on poly(allylamine hydrochloride) chains (PAH-CD) that are adsorbed on the substrate. The AD groups are fixed covalently on the AFM tip through either a one-AD or a four-AD platform linked to the tip though a PEO chain. It is found that while the rupture forces between AFM tips covered with tetravalent AD molecules and SAM-CD surfaces do not exceed twice those found with tips covered by monovalent AD molecules, the rupture forces increase by a factor of 20 on PAH-CD substrates for a tetravalent AD covered tip compared to a monovalent one. Thus, there seems to exist a synergistic effect between the molecule multivalence and the polymeric nature of the CD-covered substrate. As found in the literature, we observe an increase of the intensity of the rupture forces between the AD-covered AFM tip and the ß-CD covered substrate with the contact time over timescales up to several seconds. Finally, we find that when the host-guest system involves the multivalency of the AD guest and/or the polymeric nature of the host the mean rupture force decreases with the loading rate in contrast to what is predicted by the Bell-Evans theory. We tentatively explain this "anti-Bell-Evans" behavior by the possibility of rebinding during the rupture process. This effect should have important implications in the understanding of forces at the cellular level.

9.
J Mater Chem B ; 3(15): 3098, 2015 Apr 21.
Artigo em Inglês | MEDLINE | ID: mdl-32262510

RESUMO

Correction for 'Multivalency: influence of the residence time and the retraction rate on rupture forces measured by AFM' by Jalal Bacharouche et al., J. Mater. Chem. B, 2015, 3, 1801-1812.

10.
Biochim Biophys Acta ; 1854(2): 138-45, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25498116

RESUMO

IgG films are widely used in the field of immunoassays, especially in (double) antibody-sandwich ELISA tests where capture antibodies are coated on surfaces like polystyrene or hydrophobic self-assembled monolayers (h-SAMs). It is critical to analyze-at a molecular scale and under liquid conditions-the structure of the deposited IgG film in order to quantitatively address the efficiency of the ELISA test in terms of antigen detection. In this communication, we report an atomic force microscopy (AFM) analysis evidencing a strong relationship between immunological activities of mouse monoclonal anti-human interleukin-2 (IL-2) and 6 (IL-6) antibodies, thickness and roughness of the IgG monolayer adsorbed onto h-SAMs, and surface concentration of IgG molecules. Indirect information may be further obtained on antibody orientation. Collating the results obtained by AFM and those from ELISA tests leads us to conclude that antibodies like anti-IL-6 forming flat monolayers should be more efficient under ELISA detection conditions. In addition, the concentration of IgG in the coating suspension should be optimized to obtain a monolayer heavily populated by "end-on" adsorbed molecules, an orientation that is desirable for enhancing ELISA tests performance.


Assuntos
Anticorpos/ultraestrutura , Ensaio de Imunoadsorção Enzimática , Imunoglobulina G/química , Adsorção , Animais , Anticorpos/química , Anticorpos/imunologia , Humanos , Interações Hidrofóbicas e Hidrofílicas , Imunoglobulina G/imunologia , Imunoglobulina G/ultraestrutura , Interleucina-6/imunologia , Camundongos , Microscopia de Força Atômica , Propriedades de Superfície
11.
ACS Nano ; 7(4): 3457-65, 2013 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-23530596

RESUMO

Chemo-mechanotransduction, the way by which mechanical forces are transformed into chemical signals, plays a fundamental role in many biological processes. The first step of mechanotransduction often relies on exposure, under stretching, of cryptic sites buried in adhesion proteins. Likewise, here we report the first example of synthetic surfaces allowing for specific and fully reversible adhesion of proteins or cells promoted by mechanical action. Silicone sheets are first plasma treated and then functionalized by grafting sequentially under stretching poly(ethylene glycol) (PEG) chains and biotin or arginine-glycine-aspartic acid (RGD) peptides. At unstretched position, these ligands are not accessible for their receptors. Under a mechanical deformation, the surface becomes specifically interactive to streptavidin, biotin antibodies, or adherent for cells, the interactions both for proteins and cells being fully reversible by stretching/unstretching, revealing a reversible exposure process of the ligands. By varying the degree of stretching, the amount of interacting proteins can be varied continuously.


Assuntos
Materiais Biomiméticos/química , Mecanotransdução Celular , Membranas Artificiais , Proteínas/química , Módulo de Elasticidade , Teste de Materiais , Ligação Proteica , Proteínas/ultraestrutura , Estresse Mecânico , Propriedades de Superfície
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