Bud and Root Rot of Windmill Palm (Trachycarpus fortunei) Caused by Simultaneous Infections of Phytophthora palmivora and P. nicotianae in Sicily.

In June 2009 in a commercial nursery in eastern Sicily (Italy), 3-year-old potted windmill palms (Trachycarpus fortunei (Hooker) H. Wendl.) showed a decline in growth, wilt, droop, and basal rot of the youngest leaves. The rot progressed inward and killed the bud. Initially, older leaves remained green but eventually the entire plant collapsed. Root rot was consistently associated with aboveground symptoms. Two Phytophthora species were consistently isolated from the petiole base, heart, roots, and rhizosphere soil of symptomatic plants on a selective medium (2) and occasionally recovered from roots and rhizosphere soil of asymptomatic plants. Pure cultures were obtained by single-hypha transfers and the two species were identified on the basis of morphological and molecular characters as Phytophthora palmivora and P. nicotianae. Both species were recovered from all symptomatic plants. From multiple tissue samples per plant, we recovered either or both species. On potato dextrose agar (PDA), P. palmivora isolates grew between 10 and 35°C, with the optimum at 27°C. On V8 juice agar, they produced elliptical to ovoid, papillate, caducous sporangia (32 to 78 × 23 to 39 µm) with a mean length/breadth (l/b) ratio of 1.8:1 and a short pedicel (mean pedicel length = 5 µm). Isolates of P. nicotianae produced arachnoid colonies on PDA, grew at 37°C but did not grow at 40°C. Sporangia (29 to 55 × 23 to 45 µm) were spherical to ovoid (l/b ratio 1.3:1), papillate and often bipapillate, and noncaducous. Isolates of both species produced amphigynous antheridia and oogonia only when paired with reference isolates of P. nicotianae of the A2 mating type. The internal transcribed spacer (ITS) region of rDNA of two isolates of P. palmivora (IMI 398987 and IMI 398988) and an isolate of P. nicotianae (IMI 398989) from T. fortunei was amplified with primers ITS6/ITS4 and sequenced (1). Blast analysis of the sequences of isolates IMI 398987 and IMI 398988 (GenBank Accession Nos. HQ596556 and HQ596558) showed 99% homology with the sequence of two reference isolates of P. palmivora (GQ398157.1 and GU258862), while the sequence of isolate IMI 398989 (HQ596557) showed 99% homology with a reference isolate of P. nicotianae (EU331089.1). Pathogenicity of isolates IMI 398987 and IMI 398989 was proved by inoculating separately each isolate on 1-year-old potted plants of T. fortunei (10 plants per isolate). A zoospores suspension (2 × 104 zoospores/ml) was pipetted onto the petiole base of the three central leaves (200 µl per leaf) of each plant. Sterile water was used for control plants. All plants were incubated at 25 ± 2°C with 100% humidity for 48 h and then maintained in a greenhouse at 24 to 28°C. Within 3 weeks, all inoculated plants showed symptoms of bud rot. Control plants remained healthy. P. palmivora and P. nicotianae were reisolated only from inoculated plants. Bud rot of palms caused by P. palmivora was reported previously in Italy (3). However, to our knowledge, this is the first report of simultaneous infections of P. palmivora and P. nicotianae as causal agents of this disease. Outbreak of bud rot may have been favored by overhead sprinkler irrigation. The recovery of P. palmivora and P. nicotianae from rhizosphere soil and roots of asymptomatic plants suggests infested soil was the primary inoculum source. References: (1) D. E. L. Cooke et al. Fungal Genet. Biol. 30:17, 2000. (2) H. Masago et al. Phytopathology 67:425, 1977. (3) A. Pane et al. Plant Dis. 91:1059, 2007.

Long term effect on IOP of a stainless steel glaucoma drainage implant (Ex-PRESS) in combined surgery with phacoemulsification.

AIM: To evaluate the efficacy and safety of a stainless steel miniature glaucoma drainage device (Ex-PRESS R50) for the surgical treatment of primary open angle glaucoma (POAG) and cataract when combined with phacoemulsification. METHODS: Clinical, prospective, multicentre, single treatment arm, non-randomised, non-masked study. The Ex-PRESS device was implanted at the limbus under a conjunctival flap. Phacoemulsification cataract extraction and in the bag IOL implantation were performed through clear cornea temporally. PRIMARY OUTCOME: IOP change; secondary outcomes: side effects and VA changes. RESULTS: 26 eyes of 25 patients were implanted with the device. The mean (SD) follow up was 23.9 (10.4) months and the mean age was 75.1 (7.1) years. 17/26 eyes have more than 3 years of follow up. One case was discontinued because of device removal, one because of death, and three were lost to follow up. EFFICACY: preoperative IOP was 21 (4) mm Hg; at 1, 2, and 3 years IOP was 15.3 (3.1) mm Hg (35% reduction), 16.6 (2.7) mm Hg (29% reduction), and 16 (2.6) mm Hg (22% reduction) respectively. Kaplan-Meyer determined overall success rate (IOP < or = 21 mm Hg at the last visit with or without medications) as 76.9%. The number of antiglaucoma medications was reduced by 95% at year 1. Only six patients (23%) were taking IOP lowering treatment at their last visit, five with one medication and one with two medications. Side effects: early postoperative complications were clinically mild and included six cases of hypotony (IOP <5 mm Hg), three cases of hyphaema (<2 mm) with no clinically significant further effects. Long term complications were two cases (7.7%) of device rotation (one treated by reposition) and three cases (11.5%) of conjunctival erosion at 2 and 3 years. CONCLUSIONS: The Ex-PRESS implant, combined with phacoemulsification cataract extraction, is clinically safe and effective, maintaining in the long term a large reduction in IOP and in the number of antiglaucoma medications.

Root and Basal Stem Rot of Scotch Broom Caused by Phytophthora citricola and P. drechsleri in Italy.

Scotch broom (Cytisus scoparius (L.) Link, Fabaceae), an evergreen shrub native to Europe, is cultivated as a garden plant. In 2003 and 2004, potted plants with symptoms of leaf chlorosis, defoliation, and eventual wilt and associated with root and collar rot were observed in ornamental nurseries in Sicily. As much as 10% of plants were affected in a single nursery. Two species of Phytophthora were consistently isolated alone or together from the same pot with the selective medium of Masago et al. (2). Pure cultures were obtained by single-hypha transfers and the species were identified as P. citricola Sawada (approximately 40% of isolations) and P. drechsleri Tucker (60% of isolations) on the basis of morphological, cultural characters, and electrophoretic phenotype. The isolates of P. drechsleri grew between 10 and 37°C (optimum 27°C) on potato dextrose agar (PDA). The sporangia produced on V8 juice agar (V8A) were ellipsoid to obpyriform, nonpapillate, persistent with internal proliferation, and often forming in a sympodium. Sizes varied, 30 to 60 × 20 to 40 µm (length/width ratio between 1.4 and 2.2). The hyphal swellings were produced in aqueous culture. All isolates were A1 mating type and formed plerotic oospores (mean diameter (ф) 25 µm) with amphigynous antheridia when paired with the A2 reference isolates of P. cryptogea on V8A plus ß-sitosterol. The aryl-esterase and malate dehydrogenase isozymes of scotch broom isolates on polyacrylamide slab gels (1) were identical to those of the authentic isolate CBS 292.35 of P. drechsleri and differed from reference cultures of other nonpapillate species. The cardinal temperatures of P. citricola isolates on PDA ranged from 2 to 30°C (optimum 25°C). In liquid culture, the isolates produced irregular-shaped, obovoid to obpyriform sporangia 20 to 70 × 21 to 44 µm that were noncaducous, semipapillate or with inconspicuous papilla, often with two apices. The isolates were homothallic and produced oospores (mean ф 22 µm) with paragynous antheridia. The electrophoretic phenotype of these isolates was identical to the phenotype of P. citricola reference isolates and very different from that of the reference isolates of other semipapillate species. The pathogenicity tests of the representative isolates of P. drechsleri (IMI 391710) and P. citricola (IMI 391715) were carried out in a screenhouse. Twenty 3-month-old scotch broom seedlings were transplanted into pots (12 cm ф) filled with soil infested with the inoculum produced on a mixture of vermiculite and autoclaved oat seeds. The plants were maintained at 20 to 28°C and watered to field capacity once a week. After 30 to 40 days, all inoculated plants showed symptoms of wilting and root rot. The 20 control plants transplanted into pots containing noninfested soil remained healthy. P. citricola and P. drechsleri were reisolated from infected tissues. To our knowledge, this is the first report of P. citricola and P. drechsleri on scotch broom. A root rot of scotch broom caused by P. megasperma has been reported in central Italy (3). References: (1) S. O. Cacciola et al. Plant Dis. 86:327, 2002. (2) D. C Erwin and O. K. Ribeiro. Pages 39-41 in: Phytophthora Diseases Worldwide. The American Phytopathological Society, St. Paul, MN, 1996. (3) A. M. Vettraino and A. Vannini. Plant Pathol. 53:417, 2003.

Subfoveal choroidal neovascularization in a patient with pre-existing pseudomacular hole.

PURPOSE: To report concomitant use of fluorescein angiography (FA) and optical coherence tomography (OCT) in a case of pre-existing pseudomacular hole (PMH) and macular choroidal neovascularization (CNV) treated with photodynamic therapy (PDT). METHODS: Case report. RESULTS: A 63-year-old man who received laser treatment for branch retinal vein occlusion in 1993 developed an asymptomatic macular epiretinal membrane 2 years later. In 2001, the patient complained of metamorphopsia. Biomicroscopy showed a PMH, confirmed by OCT. One year later, metamorphopsia suddenly increased and visual acuity dropped from 20/20 to 20/70. FA and OCT revealed subfoveal occult CNV PDT was successfully performed. FA and OCT were essential diagnostic tools during the follow-up. CONCLUSIONS: The case presented stresses the importance of different tools for accurate diagnosis and proper follow-up of macular diseases.