[Oligo- and polynucleotide probes. A molecular hybridization method]. / Oligo- i polinukleotidnye zondy. Metod molekuliarnoi gibridizatsii.

In this review the main principles and possibilities of a hybridization analyses as a highly specific methods for diagnostics of infections and genetic diseases are discussed. Special attention is paid to the chemical approaches to the preparation of non-radioactive probes based on synthetic oligonucleotides and polynucleotides containing biotin, Eu3+ and enzyme labels. Some versions of the hybridization analysis technique are discussed.

[Biotinylated oligonucleotides as probes in the method of molecular hybridization]. / Mechenye biotinom oligonukleotidy kak zondy v metode molekuliarnoi gibridizatsii.

The chemical approaches to the preparation of non-radioactive and biotinylated probes based on synthetic oligonucleotides and their applicability to the molecular hybridization method are discussed.

[Template properties of the decadeoxynucleotide d(pCCACGAAACC) in the RNA polymerase system of Escherichia coli]. / Issledovanie matrichnykh svoistv dekadezoksinukleotida d(pCCACGAAACC) v sisteme RNK-polimerazy Escherichia coli.

The decadeoxynucleotide d(pCCACGAAACC) transcription by E. coli RNA-polymerase was studied. The transcript was shown to be several times longer than the template. The oligonucleotide GpGpGpGpUp complementary to the "contact" of two neighbouring template molecules was found in the pancreatic RNase digest of the RNA-product. This fact is consistent with our hypothesis reported recently. Pentanucleotide d(pGGTTT) may funtion as a primer in the decadeoxynucleotide transcription being incorporated into RNA.