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1.
J Helminthol ; 93(3): 277-285, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-29708080

RESUMO

The aim of this study was to evaluate the pattern of local immune cell infiltration in human cystic echinococcosis (CE) by identifying the subtypes of immune cells using immunohistochemistry (IHC). Fifty surgically removed hydatid cyst samples and surrounding tissues were collected from patients referred to Al-Zahra Hospital, Isfahan, Iran. IHC was performed on the surrounding host tissue of hydatid cysts using anti-human CD3, CD19, CD8, CD4, CD68, CD56, Ki-67 and Foxp3 (forkhead box P3) antibodies. The results were then compared to hepatocellular carcinoma and chronic hepatitis. In the host-tissue reaction site of liver hydatid cysts, a distinct pattern of local immune cell response, which outwardly consisted of a pack of the fibrous elements, a layer of palisading macrophages, an eosinophil-containing layer and a layer of accumulated lymphocytes, was observed. However, in some cases there were no positive cells for CD56+ natural killer cells and Foxp3+ regulatory T cells. The CD3+ T cells were the predominant inflammatory cells in all groups, followed by CD19+ B cells. It can be concluded that different immune cells are involved in the local response to human hydatid cysts.


Assuntos
Equinococose/patologia , Imuno-Histoquímica , Inflamação/patologia , Leucócitos Mononucleares/imunologia , Antígenos CD/análise , Carcinoma Hepatocelular/patologia , Equinococose/cirurgia , Fatores de Transcrição Forkhead/análise , Hepatite Crônica/patologia , Hospitais , Humanos , Irã (Geográfico) , Antígeno Ki-67/análise , Leucócitos Mononucleares/química , Leucócitos Mononucleares/classificação , Neoplasias Hepáticas/patologia
2.
J Helminthol ; 92(6): 696-702, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-29103387

RESUMO

Cystic echinococcosis (CE) is a medically important parasite-caused human disease. Humans may acquire the infection accidentally by ingestion of E. granulosus eggs. The parasite has a broad range of hosts and genotypes, which may affect its aetiological and biological characteristics. The present study aimed to determine the genetic characteristics of human isolates of E. granulosus in Isfahan, Iran. In this cross-sectional study, 50 surgically removed hydatid cysts were collected from hospitalized patients in Al-Zahra Hospital, Isfahan, Iran, over a period of 2 years (2015-2017). DNA was extracted from cyst material, and polymerase chain reactions (PCR) were performed targeting cox1 and nad1 genes. Amplicons were sequenced directly and the resulting sequences were aligned and analysed. Phylogenetic and genetic diversity analyses were also performed. Among the isolates, 43 (86%), 3 (6%) and 4 (8%) out of 50 were E. granulosus (G1), E. granulosus (G3) and E. intermedius (G6), respectively. In total, nine and eight haplotypes were identified by nad1 and cox1 gene analysis, respectively. The haplotype diversity index was higher by cox1 gene analysis (0.547) in G1 strains compared with nad1 (0.433). The G1 genotype was the most predominant isolate from human cases of CE, and the presence of G6 is indicative of an important role of camels in the development of human CE in Isfahan. This is the first report of the G3 genotype causing human CE in Isfahan. Moreover, cox1 gene analysis enables a higher resolution of the genetic diversity of the E. granulosus population compared with nad1 gene analysis.


Assuntos
Equinococose/parasitologia , Echinococcus granulosus/classificação , Echinococcus granulosus/genética , Complexo IV da Cadeia de Transporte de Elétrons/genética , Variação Genética , NADH Desidrogenase/genética , Animais , Análise por Conglomerados , Estudos Transversais , Echinococcus granulosus/enzimologia , Echinococcus granulosus/isolamento & purificação , Haplótipos , Humanos , Irã (Geográfico) , Filogenia , Reação em Cadeia da Polimerase , Análise de Sequência de DNA , Homologia de Sequência
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