RESUMO
Screening medicine is a basis of a suggested new concept; it comprises: an evaluation of the basic health fund within the terms of real time; continuity of the medical, ecological, epidemical, congenital and genetic information collected within the medical passport; as well as synthesis of the obtained data and monitoring of patients. The above concept enables a qualitatively new approach to coping with issues of individual health and to rendering a high-quality medical care in oncology.
Assuntos
Oncologia/normas , Humanos , Programas de Rastreamento , Prontuários Médicos , Monitorização Fisiológica , Qualidade da Assistência à Saúde , Sistema de RegistrosRESUMO
From 43 (35.8%) out of 120 samples of pasteurized milk used by volunteers 182 cultures of five Yersinia species--Y. enterocolitica, biovar 1 (70.3%), Y. intermedia (18.1%), Y. frederiksenii (7.7%), Y. kristensenii (3.3%) and Y. aldovae (0.5%)--were isolated in the course of bacteriological studies carried out in 1987-1990. The isolated Yersinia cultures belonged to nonpathogenic pyraminidaze-positive serovars. Yersinia pathogenic bio/serovars (4/O3, 2/O9, 1B/O8) were not detected. No deviations from the normal state were registered in the volunteers drinking pasteurized milk, and Yersinia could be isolated from their feces only during the first 3 days following milk consumption. In 390 patients examined for the presence of Yersinia infections the isolation rate of Yersinia of biovar 1 (99.6% of the cultures) varied between 4.4% and 21.3% and correlated with the increase of the isolation rate from milk (13.6% to 53.9%). The results of the study suggest that unboiled pasteurized milk contributes to the spread of Yersinia carriership among the population.
Assuntos
Laticínios/microbiologia , Microbiologia de Alimentos , Leite/microbiologia , Yersiniose/transmissão , Animais , Fezes/microbiologia , Humanos , Estações do Ano , Sorotipagem , Sibéria , Yersinia/classificação , Yersinia/isolamento & purificação , Yersiniose/microbiologia , Yersinia enterocolitica/classificação , Yersinia enterocolitica/isolamento & purificaçãoAssuntos
Contaminação de Equipamentos , Microbiologia de Alimentos , Doenças Transmitidas por Alimentos/etiologia , Refrigeração/efeitos adversos , Yersiniose/etiologia , Yersinia enterocolitica/patogenicidade , Yersinia/patogenicidade , Desinfecção/métodos , Contaminação de Equipamentos/prevenção & controle , Doenças Transmitidas por Alimentos/prevenção & controle , Humanos , Refrigeração/instrumentação , Temperatura , Yersinia/isolamento & purificação , Yersiniose/prevenção & controle , Yersinia enterocolitica/isolamento & purificaçãoRESUMO
For the first time Y. intermedia strains containing plasmids with a molecular weight of 82 MD have been detected in natural populations of urease-positive Yersinia. Such populations have been isolated from two species of birds and from the soil in the area where they have been killed (the Maritime Territory), as well as from washings from the surface of onions in a vegetable store (Chita Province). The strains, administered orally to white mice, proved to be nonpathogenic. Plasmids with a molecular weight of 82 MD are supposed to occur in natural populations of other Yersinia species.
Assuntos
Reservatórios de Doenças , Plasmídeos , Yersinia/isolamento & purificação , Allium/microbiologia , Animais , Aves/microbiologia , Microbiologia de Alimentos , Camundongos , Peso Molecular , Sorotipagem , Sibéria , Microbiologia do Solo , Yersinia/classificação , Yersinia/patogenicidadeRESUMO
A modified nutrient medium, made of reagents and standard media manufactured in this country, tried in practice, is suggested for Yersinia pyrazinamidase test. Tisamid (Finland), a pyrazinamide antituberculosis agent was used as substrate. A positive test is characteristic of nonpathogenic and a negative one for pathogenic Yersinia. The test and the medium may be effectively used in laboratory diagnostic studies for identification of Yersinia.
Assuntos
Amidoidrolases/metabolismo , Yersinia/enzimologia , Yersinia/classificaçãoRESUMO
The clones of two types (T+ and T-) have been identified among the strains of Y. pseudotuberculosis. The difference in the morphology of the clones is based on the ability of T+ clones to agglutinate in the process of growth. The identified clones are different in calcium dependence, in the ability to agglutinate and in their virulence for the laboratory animals. The differences have been proved to be connected with the presence of a 45 Md plasmid in T+ cells. Replication of this plasmid is suppressed by the acridine orange dye in concentrations of 12.5 or 25.0 mkg X ml-1. The plasmid is spontaneously lost from the strains during their continuous storage. The microscopy of colonies permits the selection of clones with the parental phenotype from the populations having lost the 45 Md plasmid.