Low serum levels of zinc and 25-hydroxyvitmain D as potential risk factors for COVID-19 susceptibility: a pilot case-control study.

BACKGROUND AND AIMS: This study aimed to evaluate serum 25-hydroxyvitmain D and zinc levels in coronavirus disease 2019 (COVID-19) patients in comparison to healthy subjects. METHODS: This was a single-center case-control study performed from March 20, 2020, to January 20, 2021, in Tehran, Iran. All patients diagnosed with COVID-19 based on a positive nasopharyngeal swab polymerase chain reaction (PCR) test were included in the case group. Controls were selected from patients referred for routine checkups who had a negative COVID-19 PCR test. Age, sex, marital and educational status, comorbidities, and serum 25-hydroxyvitmain D and zinc levels of patients were recorded. RESULTS: Ninety patients in the case group and 95 subjects in the control group who were sex and age-matched were studied. 25-hydroxyvitmain D levels higher than 20 ng/ml were observed in 58 (64%) cases and 72 (76%) controls (P = 0.09). The median 25-hydroxyvitmain D level in the case group was significantly lower than controls (26 (interquartile range [IQR] = 24) ng/ml vs. 38 (IQR = 22) ng/ml, respectively, P < 0.01). The median zinc level in the case group was 56 (IQR = 23) mcg/dL, while it was 110 (IQR = 27) mcg/dL among the controls (P < 0.01). There was no significant difference in the level of 25-hydroxyvitmain D and zinc between cases with and without comorbidities (P > 0.05). Susceptibility to SARS-CoV-2 infection could be predicted by serum 25-hydroxyvitmain D levels below 25.2 ng/ml (81% sensitivity; 48% specificity) or zinc levels below 86.3 mcg/dL (93% sensitivity; 92% specificity). CONCLUSIONS: Low serum zinc and 25-hydroxyvitmain D levels appear to be risk factors for COVID-19 affliction; thus, the treatment of individuals with such deficiencies is recommended.

Efficacy and safety of topical spironolactone 5% cream in the treatment of acne: A pilot study.

BACKGROUND: Spironolactone is an effective treatment for female patients with acne vulgaris. However, topical spironolactone could be a valuable treatment option in both male and female acne patients due to the less possibility of systemic side effects with its topical formulation. OBJECTIVE: To evaluate the efficacy and safety of 5% spironolactone cream in the treatment of mild to moderate acne vulgaris. METHODS: In this pilot clinical trial, topical spironolactone 5% was evaluated to treat patients with mild to moderate acne twice a day for 8 weeks. The rate of improvement as any alterations in the number of open and closed comedones, facial inflammatory papules, and acne global grading scores were assessed. Moreover, skin biometric characteristics including skin hydration, erythema, transepidermal water loss (TEWL), pH, sebum, and Propionibacterium acnes bacteria activity were also assessed following the treatment. RESULTS: Fifteen patients participated in our study with a mean age of 25 ± 4.87 years old. A total of 66.6% (n = 10) were female and 33.4% (n = 5) were male. The number of acne papules, open and closed comedones, and acne global grading score decreased significantly 4 and 8 weeks after the beginning of treatment (P < .05). No considerable side effect was reported. Moreover, there was no significant difference between the skin hydration, melanin, erythema, TEWL, pH index, sebum, and P acnes bacteria activity before, 4, and 8 weeks after the treatment with topical spironolactone cream (P > .05). CONCLUSION: The topical 5% spironolactone cream seems to be an effective and safe treatment of acne vulgaris in both male and female patients.

Comparative Effect of Nature-Based Sounds Intervention and Headphones Intervention on Pain Severity After Cesarean Section: A Prospective Double-Blind Randomized Trial.

BACKGROUND: Non-pharmacological treatment methods are being increasingly investigated for pain prevention and relief either alone or in combination with pharmacological treatment. METHODS: The present randomized placebo-controlled trial was conducted on 57 mothers undergoing elective cesarean section over 10 months from April 2015 to February 2016. The participants were randomly assigned to three groups: control, headphone, and nature-based sounds (N-BS). The investigator recorded pain severity every eight hours after the surgery. Mothers in the headphone group used headphones for 20 minutes (without playing sounds) and mothers in the N-BS group used headphones and listened to N-BS for 20 minutes. We played pleasant nature sounds for the N-BS group using media players and headphones. Mothers' pain severity was measured immediately before the intervention and 15 and 60 minutes after the end of the intervention. RESULTS: The N-BS group had a significantly lower pain severity than the headphone and control groups. Statistically insignificant differences were observed between the control and headphone groups indicating that headphone only did not reduce the pain in the intervention group. These reductions were more evident progressively in 15 and 60 minutes after the end of the intervention. CONCLUSIONS: The application of N-BS for mothers undergoing elective cesarean section promotes nursing autonomy and the notion that nurses can influence the patient's environment.

The Zinc and Copper Levels in Thalassemia Major Patients, Receiving Iron Chelation Therapy.

Essential trace elements deficiency including zinc and copper are frequently reported in the literature, but the results are controversial. The aim of this study was to evaluate zinc and copper levels in thalassemia major (TM) patients who were on regular transfusion and iron chelation therapy. In a case-control cross-sectional study 43 TM patients and 43 age-matched and sex-matched healthy controls were examined. Patients were selected by convenience sampling method from TM patients who were registered in Thalassemia Clinic during 6 months. Serum zinc and copper levels were evaluated in all subjects. Zinc and copper dietary intake were also assessed. The median zinc level in the participants was significantly lower than the control group (35 [6.3 to 298] vs. 173 [3.1 to 584] µg/dL; P<0.05), but the mean copper level was significantly higher in the patients in comparison with the control group (260±118 vs. 201±69 µg/dL; P<0.05). In contrast, the mean zinc and copper dietary intake in the patient's group were significantly lower than the control group. The mean serum zinc and copper levels in the patient's group were not different according to iron chelation therapy type. Also, zinc and copper levels in the patient's group were not statistically significant based on ferritin level, age, and duration of therapy. Essential trace elements level change and deficiency might occur in TM patients. Hence, routine assessment of these elements is recommended for better management.