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Biull Eksp Biol Med ; 109(6): 577-8, 1990 Jun.
Artigo em Russo | MEDLINE | ID: mdl-2397298

RESUMO

Histofluorescence technique using glyoxylic acid revealed a specific fluorescence suggesting the presence of biogenic monoamines in early developmental stages of CBA x C57 Black mice. A yellow fluorescence observed in the blastomere surface from the stage of zygote up to that of four blastomere points to the presence of indole derivates. As development proceeds, the fluorescence increases and its colour becomes more and more green, which is characteristic of catecholamines. From the stage of eight blastomeres up to stage of blastocyst specific fluorescence is revealed in the cytoplasm. The inhibitors of monoamine oxidase, introduced into pregnant mice, markedly increased the specific fluorescence. An assumption is made of functional activity of biogenic monoamines in early mouse embryos.


Assuntos
Monoaminas Biogênicas/análise , Embrião de Mamíferos/análise , Desenvolvimento Embrionário , Óvulo/análise , Animais , Blastocisto/análise , Blastômeros/análise , Catecolaminas/análise , Citoplasma/análise , Feminino , Fluorescência , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Monoaminoxidase/administração & dosagem , Monoaminoxidase/farmacologia , Óvulo/citologia , Gravidez
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