RESUMO
Histamine has been reported to cause endothelium-dependent relaxation of vascular smooth muscle and vasodilation. This study was undertaken to examine the inhibitory effects of histamine on cylindrical segments of extrapulmonary arteries isolated from male Sprague Dawley rats. In arterial segments precontracted with phenylephrine (10 microM), histamine (0.1-100 microM) elicited concentration-dependent relaxation responses. Removal of the endothelium or pretreatment with methylene blue (10 microM) abolished relaxation responses to low concentrations of histamine and markedly inhibited those caused by histamine at concentrations greater than 1 microM. Incubation of endothelium-intact arterial segments with pyrilamine (1 microM) caused a significant rightward shift of the histamine concentration-response curves. Treatment of the segments with cimetidine (100 microM) or indomethacin (10 microM) only minimally antagonized histamine-induced relaxation in arteries with endothelium. Residual relaxation responses observed in arteries stripped of endothelium were unaffected by pretreatment with cimetidine, indomethacin, or pyrilamine. The results suggest that the inhibitory effect of histamine in rat pulmonary arteries is mediated predominantly by activation of H1-receptors on the endothelium and the subsequent release of endothelium-derived relaxing factor(s).
Assuntos
Endotélio Vascular/fisiologia , Histamina/farmacologia , Fenilefrina/farmacologia , Artéria Pulmonar/efeitos dos fármacos , Receptores Histamínicos H1/metabolismo , Vasodilatação/efeitos dos fármacos , Animais , Cimetidina/farmacologia , Indometacina/farmacologia , Masculino , Músculo Liso Vascular/efeitos dos fármacos , Óxido Nítrico/metabolismo , Artéria Pulmonar/fisiologia , Pirilamina/farmacologia , Ratos , Ratos EndogâmicosRESUMO
The influence of endothelium on angiotensin II-induced contraction was investigated in rings of rat aorta, bovine coronary artery, bovine intrapulmonary artery and bovine intrapulmonary vein. Destruction of endothelium significantly enhanced angiotensin II-induced contraction in rat aorta and bovine coronary artery, but not in bovine intrapulmonary artery and bovine intrapulmonary vein. Indomethacin (10(-5) M) did not alter angiotensin II-induced contraction in rat aorta or bovine coronary artery. However, hemoglobin (10(-5) M) or methylene blue (10(-5) M) significantly enhanced angiotensin II-induced contraction in rat aorta and bovine coronary artery with, but not without, endothelium. Intimal rubbing did not affect stimulation of phosphoinositide hydrolysis by angiotensin II in rat aorta. The findings demonstrate that angiotensin II-induced contraction in vascular rings can be modulated by endothelium. However, the effect of endothelium apparently depends upon the species and vascular bed from which the vessel is isolated. Results obtained using inhibitors suggest that in rat aorta and bovine coronary artery release of endothelium-derived relaxant factor (EDRF), rather than cyclooxygenase products, is involved in mediating the inhibitory influence of endothelium. Further, similar stimulation of phosphoinositide hydrolysis in intimally rubbed and unrubbed rat aorta suggests that EDRF does not modulate angiotensin II-induced contraction in this vessel by inhibiting angiotensin II stimulation of phosphoinositide hydrolysis.
Assuntos
Angiotensina II/farmacologia , Endotélio Vascular/fisiologia , Músculo Liso Vascular/efeitos dos fármacos , Animais , Bovinos , Hemoglobinas/farmacologia , Hidrólise , Técnicas In Vitro , Indometacina/farmacologia , Contração Isométrica/efeitos dos fármacos , Azul de Metileno/farmacologia , Contração Muscular/efeitos dos fármacos , Fosfatidilinositóis/metabolismo , Ratos , Ratos EndogâmicosRESUMO
In this study, slices of rat anterior pituitary were prelabeled with [(3)H]myo-inositol and the ability of angiotensins II and III to stimulate [(3)H]phosphoinositide hydrolysis was characterized. When using tissue derived from ovariectomized rats, dose-response experiments revealed that angiotensin II significantly increases [(3)H]inositol monophosphate formation (in the presence of 10 mM LiCI) at concentrations of 10 nM and above. Maximal stimulation by angiotensin II was observed at 1 ?M (228% of basal) and 50% maximal stimulation was at 10.8 +/- 2.7 nM. Angiotensin III was less potent when compared to angiotensin II (maximal stimulation at 10 ?M; 220% of basal: 50% maximal stimulation, 475 +/- 159 nM). When using normal female rats, significant stimulation by angiotensin II was not observed until 1 ?M angiotensin II. When ovariectomized rats were treated for 7 days with 17?-estradiol, increases in [(3)H]inositol monophosphate induced by 1 ?M angiotensin II were significantly reduced when compared to sesame oil vehicle controls. This study shows that estrogen down-regulates angiotensin receptor coupling in the anterior pituitary. Moreover, it illustrates the influence of the hormonal state of the animal on the regulation of the effects of angiotensins in this tissue.