Comparative effect of micro-osteoperforation and Photo-biomodulation on the rate of maxillary canine retraction: A split mouth randomized clinical trial.

INTRODUCTION: The lengthy duration of comprehensive orthodontic treatment has always been a major concern. Various modalities have been proposed to accelerate the orthodontic tooth movement (OTM) with varying degree of success. However, there is limited literature, comparing the efficiency of two commonly used modalities which are relatively simple, safe and comfortable chair-side procedures to accelerate orthodontic tooth movement viz. Photo-biomodulation (PBM) being non-invasive and micro-osteoperforations (MOPs) being minimally invasive. The aim was to evaluate the comparative effect of PBM and MOPs on the rate of orthodontic tooth movement (OTM). METHODS: In this split mouth study, thirty patients requiring ma-xillary first premolar extraction were randomly allocated for Photo-biomodulation (PBM) on one side and for micro-osteoperforations (MOPs) on other side of maxilla. Separate canine retraction was performed and movement was measured over a three month period. RESULTS: Micro-osteoperforations (MOPs) significantly increased the rate of maxillary canine retraction by 1.1 fold when compared to the Photo-biomodulation (PBM).The MOPs can successfully reduce the comprehensive fixed orthodontic treatment time by around 9% as compared to PBM. CONCLUSIONS: Micro-osteoperforation is a more efficient modality of accelerating tooth movement as compared to Photo-biomodulation which can be used in routine orthodontic practice.

A statistical approach to optimization of fermentative production of poly(gamma-glutamic acid) from Bacillus licheniformis NCIM 2324.

This paper reports on the optimization of poly(gamma-glutamic acid) (PGA) production by Bacillus licheniformis NCIM 2324 using a statistical approach. One-factor-at-a-time method was used to investigate the effect of carbon sources, nitrogen sources and pH on PGA production. Plackett-Burman design was adopted to select the most important nutrients influencing the yield of PGA. After identifying effective nutrients, response surface methodology was used to develop a mathematical model to identify the optimum concentrations of the key nutrients for higher PGA production, and confirm its validity experimentally. PGA production increased significantly from 5.27 to 26.12 g/l when the strain was cultivated in the optimal medium developed by using statistical approach, as compared to basal medium.

Enhanced production of poly (gamma-glutamic acid) from Bacillus licheniformis NCIM 2324 in solid state fermentation.

This work reports on the optimization of PGA production by Bacillus licheniformis NCIM 2324 in solid state fermentation (SSF). In the first step, the one factor-at-a-time method was used to investigate the effect of solid substrates, initial moisture content, pH, and additional carbon and nitrogen source on PGA production; subsequently, response surface methodology (RSM) was used to establish the optimum concentrations of the key nutrients for higher PGA production. In the second step, the effects of amino acids and TCA cycle intermediates on the production of PGA were studied. The final optimized medium gave a maximum yield of 98.64 +/- 1.61 mg gds(-1) of PGA, which is significantly higher than that reported in the literature.