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Electron. j. biotechnol ; Electron. j. biotechnol;8(3)Dec. 2005. graf
Artigo em Inglês | LILACS | ID: lil-448795

RESUMO

High performance enzymatic synthesis of oleyl oleate, a liquid wax ester was carried out by lipase-catalysed esterification of oleic acid and oleyl alcohol. Various reaction parameters were optimised to obtain high yield of oleyl oleate. The optimum condition to produce oleyl oleate was reaction time; 5 min, organic solvents of log P is greater than or equal to 3.5, temperature; 40-50 ºC, amount of enzyme; 0.2-0.4 g and molar ratio of oleyl alcohol to oleic acid; 2:1. The operational stability of enzyme was maintained at >90 percent yield up to 9 cycles. Analysis of the yield of the product showed that at optimum conditions, >95 percent liquid wax esters were produced.


Assuntos
Ácidos Oleicos/biossíntese , Candida/enzimologia , Lipase/metabolismo , Ácidos Oleicos/química , Esterificação , Enzimas Imobilizadas/metabolismo , Ésteres/metabolismo , Lipase/química , Solventes , Especificidade por Substrato , Temperatura , Fatores de Tempo
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