RESUMO
Whether the MDCK cell line might adapt to grow in serum-free Hybris-2 medium and influenza viruses might be reproduced in the adapted cells was studied. Seventeen passages using the Hybris-2 medium yielded cells adapted to growth in this medium (MDCK-BS). The reproduction of influenza A (H1N1 and H3N2) and B viruses versus the cells cultured in Eagle's medium was studied. The laboratory strain of influenza A/Aichi/1/68 (H3N2) and the strain B/Ohio/01/05 of influenza B in equal titers were shown to be reproduced in both control cells on Eagle's medium and MDCK-BS cells adapted to growth in the Hybris-2 medium. The reproduction of the strains A/Brisbane/10/07 (H3N2) and A/Solomon Islands/3/06 (H1N1) was less active in the MDCK cells. Each strain of influenza viruses displayed varying infective activities. The developed serum-free Hybris-2 medium may be used for cultivation of monolayer continuous MDCK cells and for their reproduction of influenza A and B viruses.
Assuntos
Vírus da Influenza A/crescimento & desenvolvimento , Vírus da Influenza B/crescimento & desenvolvimento , Cultura de Vírus/métodos , Animais , Linhagem Celular , Meios de Cultura Livres de Soro , Cães , Replicação ViralRESUMO
Effects of exogenous bioresonance oscillations on biological and morphological characteristics of continuous human lung and liver cells were studied. Proliferative activities and viability of cells decreased after exposure to frequencies of 8 and 78.5 Hz. This was paralleled by cytopathic disorders in lung and liver cells. The frequency of 72 Hz exhibited a less intense effect on both cell types. Cell contamination with mycoplasmas was provisionally suppressed after cell exposure according to F44 and F45 programs and to 97.5 and 69.5 Hz frequencies.
Assuntos
Linhagem Celular/efeitos da radiação , Fígado/citologia , Pulmão/citologia , Radiação , Linhagem Celular/microbiologia , Linhagem Celular/ultraestrutura , Proliferação de Células , Forma Celular , Humanos , Mycoplasma/efeitos da radiação , PeriodicidadeRESUMO
Candida infection caused by intravenous or intracerebral contamination with a clinical strain of C. albicans 1755 was studied comparatively on 230 albino mice. The contamination doses ranged from 10(6) to 4 . 10(7) CFU/mouse. The developing infection could be characterized as Candida encephalomeningitis complicated by generalized candidiasis. Both the contamination routes mainly led to affections of the brain, kidneys and heart. The same distribution pattern of the pathogen was observed when the culture killed by heating was administered. The intracerebral route had advantages in chemotherapeutic studies since it induced less severe and more prolonged infection. Acute purulent inflammation of the brain and kidneys developing immediately after the contamination by days 5 to 6 was replaced by a granulomatous reaction and fibroplastic processes. Decreased acute inflammation along with changes in the nature of the pathogen vegetation and morphotinctorial properties in the affected organs can be used as a criterion of the antimycotic agent efficacy. A system for estimating pathomorphological changes in the tissues and the pathogen state is described and its use is illustrated with application of amphotericin B, mycoheptin and 5-phthorcytosine.
Assuntos
Candidíase/patologia , Anfotericina B/farmacologia , Animais , Antifúngicos/farmacologia , Encéfalo , Candidíase/etiologia , Contagem de Colônia Microbiana , Modelos Animais de Doenças , Flucitosina/farmacologia , Injeções , Injeções Intravenosas , Camundongos , Polienos/farmacologiaRESUMO
Candida infection caused by intracerebral administration of collection and clinical strains of C. albicans was studied on 1000 albino mice. The infecting doses ranged from 5 X 10(6) to 200 X 10(6) cells. Developing infection might be characterized as Candida encephalomeningitis complicated by generalized candidiasis. Acute purulent inflammations in the tissues of the brain, kidneys and myocardium developing at early periods after the infection were replaced by granulomatous reaction and fibroplastic processes. Generalization of the infection was confirmed by C. albicans isolation from blood and internal organs. Development of multiple microabscesses in the brain tissue and early severe affections in the kidneys and myocardium were a pathomorphological characteristics of the infectious process due to administration of a clinical virulent strain of C. albicans to the brain. Intracerebral administration of various C. albicans strains to mice provided their differentiation by the virulent properties.