RESUMO
Coronin, described in organisms from yeasts to humans, has been found to be involved in various actin-associated activities. It has yet to be described in Acanthamoeba, medically significant as the causative agent of granulomatous amebic encephalitis and amoebic keratitis and used extensively in actin-related studies. We isolated and characterized a cDNA encoding a coronin-like protein in A. healyi by sequence analysis and demonstrated intracellular localization of the gene product by transient transfection. Named Ahcoronin, the gene is composed of 454 amino acids which contain the characteristic WD repeats of coronin and coronin-like proteins. The C-terminal region of the gene was also predicted to have a high tendency of forming a coiled-coil, another structural characteristic of coronin. The gene showed a 50% homology to coronins. Ahcoronin occurs as a single copy and expressed as a transcript of approximately 1.4kb in A. healyi. Results of transfection showed that Ahcoronin was localized in the cell's periphery and in the leading edge consistent to that of actin. The fusion protein has also been observed to localize around phagocytic cups but was disassembled later during phagocytosis. Sequence analysis of Ahcoronin homologue of A. healyi showed numerous potential for further studies and is sure to contribute in the growing interest toward the properties and functions of coronin and coronin-like proteins.
Assuntos
Acanthamoeba/genética , Actinas/genética , DNA de Protozoário/química , Proteínas dos Microfilamentos/genética , Proteínas de Protozoários/genética , Acanthamoeba/química , Acanthamoeba/classificação , Actinas/química , Sequência de Aminoácidos , Animais , Sequência de Bases , Northern Blotting , Southern Blotting , Clonagem Molecular , DNA Complementar/química , Vetores Genéticos , Proteínas de Fluorescência Verde/análise , Humanos , Proteínas dos Microfilamentos/química , Microscopia de Fluorescência , Dados de Sequência Molecular , Fagocitose , Filogenia , Proteínas de Protozoários/química , Alinhamento de Sequência , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , TransfecçãoRESUMO
A small scale survey was performed to know the infection status of intestinal parasite in children of the residential institutions and street communities in Metro Manila, Philippines. A total of 284 stool samples from 11 institutions and 3 street communities was examined by the formalin-ether concentration method. The scotch tape anal swab was adapted to 121 children to investigate the infection status of Enterovius vermicularis. It was found out that 62.0% of the children examined were positive for one or more intestinal parasites. Multiple infections were observed in 34.2% of the children. Among 172 children who gave detail information, the prevalence for Ascaris lumbricoides, Trichuris trichiura, and hookworm was 36.0%, 44.8%, and 7.0% respectively. Of the children examined, 47.7% were found to be harboring parasitic protozoans such as Entamoeba histolytica, Giardia lamblia, and Blastocystis hominis. The most prevalent of these protozoans was B. hominis with an infection rate of 40.7%. The prevalence of these infections among children living in institutions was relatively high. More efforts should be made to implement anthelminthic programs including bi-annual follow-up treatments.
