RESUMO
Colorimetric tetrazolium assays are used increasingly in studies of fungi, often in the absence of standardization or correlation with other methods. We examined species- and strain-related tetrazolium metabolism in Candida albicans and Candida parapsilosis by using XTT [2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide] and WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulphonyl)-2H-tetrazolium] and found marked variations. Also, significant signal was often missed in the absence of dimethyl sulfoxide extraction.
Assuntos
Candida/crescimento & desenvolvimento , Candida/metabolismo , Divisão Celular , Colorimetria/métodos , Humanos , Sais de Tetrazólio/metabolismoRESUMO
A 65-year old diabetic male presented with progressive bone destruction of thoracic spine (T-11&12) with cord compression. Candida albicans was isolated from aspirated materials pre-and intra-operative. Two weeks of fluconazole was given prior to surgical debridement, and fixation of the lesion. C. albicans isolated pre-and 2-weeks after fluconazole treatment were DNA-typed using AP-PCR. MIC was 2-4 mg/l in all isolates tested. The pre-and post treatment isolates had two DNA patterns, indicating the existence of two different strains. Surgical treatment was necessary for patient recovery.
Assuntos
Antifúngicos/uso terapêutico , Candida albicans/genética , Candidíase/terapia , Fluconazol/uso terapêutico , Osteomielite/terapia , Espondilite/terapia , Vértebras Torácicas , Idoso , Candidíase/complicações , Candidíase/microbiologia , Impressões Digitais de DNA , DNA Fúngico , Desbridamento , Diabetes Mellitus Tipo 1/complicações , Humanos , Masculino , Osteomielite/complicações , Osteomielite/microbiologia , Compressão da Medula Espinal/complicações , Espondilite/complicações , Espondilite/microbiologiaRESUMO
We report various pulmonary complications occurring in 88 patients with sickle cell disease: 44 Hb SS, 29 Hb Sb and 15 Hb SC. Pulmonary infections were observed in 20% of patients, and initiated the disease in 7% of cases. They were common in children younger than 10 years especially in Hb SS and Hb Sb patients. Bacteria were identified in 50% of cases predominantly S. pneumoniae, Mycoplasma pneumoniae, and Haemophilus influenzae. Acute chest syndrome occurred in adolescents and adults and was the consequence of pulmonary infarct or embolism which should be differentiated from bacterial pneumonia. Restrive syndrome was present in 2/3 of patients. It was associated to hypoxemia in 80% of cases. Prophylactic therapy was of paramount importance on long-term penicillin therapy and on closely-spaced immunization against pneumococci and Haemophilus influenzae. Blood exchange are indicated in severe hypoxemia.
