1.
Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction.
Euro Surveill
; 17(39)2012 Sep 27.
Artigo
em Inglês
| MEDLINE
| ID: mdl-23041020
RESUMO
We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.56.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.