RESUMO
F2 promoter from corynephage BFK20 was isolated and characterized. It was functional in Escherichia coli and Corynebacterium glutamicum. Cloning of the F2 promoter into the pJUP05 promoter probe vector caused an increase of the neomycin phosphotransferase II specific activity. According to the Northern blot hybridization the nptII gene was expressed from the cloned F2 promoter. The apparent transcription start point in E. coli and C. glutamicum was determined. The -35 region of F2 promoter showed high similarity to that of E. coli promoter consensus sequence, but its -10 region was G+C rich and had no significant homology to that.
Assuntos
Bacteriófagos/genética , Corynebacterium/virologia , Genes Virais , Regiões Promotoras Genéticas , Sequência de Bases , Clonagem Molecular , Corynebacterium/genética , DNA Viral/genética , Escherichia coli/genética , Genes Bacterianos , Dados de Sequência Molecular , Plasmídeos/genética , Mapeamento por Restrição , Homologia de Sequência do Ácido Nucleico , Transcrição GênicaRESUMO
Bacteriophage BFK20 was isolated from a Brevibacterium flavum strain that had become contaminated during industrial fermentation. BFK20 has a polyhedral head 50 nm wide and a non-contractile tail 200 nm long and 10 nm in diameter. The genome of this bacteriophage consists of a linear double stranded DNA molecule of 44-45 kb with cohesive ends. The capsid of phage BFK20 contains nine polypeptides with molecular masses from 22.0-108.0 kDa. BFK20 DNA was used as a donor for fragments carrying promoters and transcription-terminators.
Assuntos
Bacteriófagos/genética , Brevibacterium , Bacteriófagos/metabolismo , Bacteriófagos/ultraestrutura , Eletroforese em Gel de Poliacrilamida , Genoma Viral , Microscopia Eletrônica , Regiões Promotoras Genéticas , Mapeamento por Restrição , Transcrição GênicaRESUMO
Walthard inclusions are described, which are frequent found like small nodules or cysts on the serosa of fallopian tubes and uterine ligaments under routine pelviscopy. The importance of Walthard inclusions is emphasized for distinguishing from the tubercles. During the three years period the incidence of Walthard inclusions from the 574 pelviscopic examinations was 19.7%.
