[Long-term storage of industrial microbial strains].

Morphofunctional characteristics of industrial microorganisms belonging to different genera, species, and strains were investigated after 15 to 20 years of storage in liquid nitrogen. The taxonomic position of microorganisms, the cell physiological state prior to storage, and the cryoconservation regime were found to affect microbial cryoresistance. Protective media, density of cell suspensions, freezing rate, and heating temperature are the parameters important for development of efficient technologies for cryoconservation of industrial microorganisms at -196 degrees C.