[A karyotype study of the Vero cell line cultured long term in a monolayer by static and roller methods]. / Issledovanie kariotipa kletok linii Vero, dlitel'no kul'tiviruemykh v monosloe staticheskim i rollernym sposobami.

A cytogenetic investigation of Vero cells, before and after adaptation to the medium containing a cattle serum, was carried out by methods of differential chromosome staining. Under these conditions, both the modal number of chromosomes (from 58 to 55) and the karyotype structure, namely the copy number of normal chromosomes and the marker composition were shown to change. The Vero cell karyotype stability was studied in the continued culture by the static (50 passages) and roll-bottle (37 passages) methods. The quantitative changes (the rising percentage of diploid cells, and the change of cell fraction involving the modal number of chromosomes) were shown to occur in spite of the chromosome composition stability, which limits the time of using Vero cells as a substrate for preparation of vaccines.

[Cytogenetic characteristics of the cell line BHK-21 (C-13) and three of its clones]. / Tsitogeneticheskaia kharakteristika kletochnoi linii BHK-21 (C-13) i trekh ee klonov.

Cell clones (C-9, C-24, C-36) of the cell line BHK-21 (C-13) were obtained and characterized. Compared to the original line, cells of these clones are larger in size, display an increased content of nuclear DNA and have more chromosomes. The G-bands were obtained by Giemsa staining following mild trypsin treatment. Marker chromosomes were determined in the original cell line BHK-21 (C-13) and in its large-cell clones. All the clones obtained are polyploid.

[The protein-synthesizing apparatus of a BHK-21 (C-13) cell culture and of its large-cell clone C-9]. / Issledovanie beloksinteziruiushchego apparata kul'tury kletok VNK-21 (C-13) i ee krupnokletochnogo klona C-9.

Cell line BHK-21 (C-13) and its large cell clone C-9 differ in morphology, karyotype and cultural properties. Clone C-9 is polyploid. It has been shown that C-9 clone cells display 2.5-3-fold excess in the nucleolus organizer region (NOR) in chromosomes, and 2-3 times higher intensity of protein synthesis and of ribosomal material content compared to the original line. Data of sedimentation analysis and of protein synthesis activity of the total ribosomal material in the cell-free translational system from rabbit reticulocytes allow to conclude that the quantity and size of polyribosomes in C-9 cells are higher than in cells of the original line. Apparently, the quantity of NOR-chromosomes reflect the activity of protein translation system of investigated cells.