A Cross-Sectional Questionnaire-Based Survey on Blood-Borne Infection Control among Romanian Dental Students.

Background and Objectives: According to the CDC estimates, 5.6 million healthcare workers worldwide are exposed to the risk of occupationally contracting blood-borne pathogens, including human immunodeficiency virus (HIV), hepatitis B virus (HBV), hepatitis C virus (HCV), and others. The aim of the present study was to assess the knowledge level on blood-borne infection control (IC) among Romanian undergraduate dental students. Materials and Methods: A cross-sectional, questionnaire-based survey with 21 items was conducted from May 2022 to June 2022. The study had α = 0.620 and Cronbach's Alpha = 0.660. The Chi-square test was used for data comparison. The data were analyzed using IBM SPSS version 26 (IBM, Armonk, NY, USA), and p ≤ 0.05 was considered statistically significant. Results: The study sample included 207 subjects with a mean age of 21.38 (±1.9) years, 59.9% F (female), 40.1% M (male), 38.2% students from year II and 61.8% from year III. Most of the subjects did not have knowledge on the indirect mode of IC, the persistence of HBV, HCV, or HIV, or the existence of rapid tests (p < 0.05). PEP (post-exposure prophylaxis) was known as follows: HBV, 32.36% (67); HCV, 25.60% (53); and HIV, 36.71% (76); p < 0.05. Only 50.24% (104) had knowledge on the recommended testing moments (p = 0.019 by gender, p = 0.752 by year of study). The optimal time to access PEP was considered by 28.01% (58), p ˃ 0.05. Only 37.68% (78) strongly agreed that the one-hand covering technique of the needle was efficient in IC (p < 0.05). Conclusions: The evaluation of the knowledge regarding IC highlighted major gaps in the perception of the subjects, which underlined the need to implement sustained forms of continuing medical education on this topic.

Evaluation of the Efficiency of Hand Hygiene Technique with Hydroalcoholic Solution by Image Color Summarize.

Background and Objectives: The HH (hand hygiene) technique is relatively simple. Even so, in practice there are still non-conformities regarding this aspect. Lack of knowledge or lack of compliance can be reasons for non-adherence with HH techniques. In this context, the purpose of this study was to follow the realization of the hand-washing technique with hydroalcoholic solution, applied before and after receiving the HH recommendations. Materials and Methods: A descriptive, cross-sectional study was conducted from May 2022 to June 2022. Students from a second year dental medicine class teaching in French 2021/22 "Grigore T. Popa" University of Medicine and Pharmacy in Iasi were included in the study. The approval of the ethics commission was received: No. 184/05.05.2022. The study was conducted in two stages. In the first stage, HH was performed without any recommendation. In the second stage, the antiseptic hand rubbing technique was presented following the WHO recommendations. The fluorescent Fluo-Add solution, Wood lamp for dermatology (4 × 5.5 W ultraviolet tubes with a wavelength of 360 nm), and photo camera were used. Subjects performed their HH movement before and after receiving instructions according to WHO. Images were initially taken from the backhand and palm and were finally analyzed with Image Color Summarizer. The data were analyzed by the Mann-Whitney U Test, t-test paired samples using IBM-SPSS version 26 (IBM, Armonk, NY, USA), and p ≤ 0.05 was considered statistically significant. Results: After analyzing the images, there were 70 subjects, 45.7% (32) female and 54.33% (38) male. Final average covered area of backhand was 60.89% (±17.17), 28.84:86.11, compared to 52.07% (±17.04), 9:85.23. Final average covered area for palm was 69.91% (±13.5), 31.61:93.41 compared to 59.74% (±16.64), 26.13:92.72. No statistical significance was obtained by gender. Conclusions: The study showed an improvement in hand hygiene technique without highlighting gender differences.

Title Cross-Sectional Study to Evaluate Knowledge and Attitudes on Oral Hygiene of Romanian Students.

Background and Objectives: the purpose of this study was to evaluate students' level of knowledge and attitude towards oral hygiene. Materials and Methods: the evaluation was carried out by a questionnaire, with 30 Q (questions) as follows: demographic data (Q1-Q5), oral hygiene knowledge data (Q6-Q23) and oral hygiene attitude data (Q24-Q30). The study included students from Romanian schools and the selection of the study group was made following selection criteria in accordance with ethical issues. A descriptive statistical analysis was performed and a value of p ≤ 0.05 was considered statistically significant. Results: the study included a number of 718 subjects with a mean age of 14.54 (±2.22), male 250 (34.8%) and female 468 (65.2%), MS (middle school students) 354 (49.4%) and HH (high school students) 364 (50.6%). Most of the subjects 292 (MS = 160; HS = 132) know a toothbrushing technique, p = 0.009, r = 0.091 and 587 (MS = 278; HS = 309) know that brushing removes the bacterial plaque p = 0.027, r = -0.082 but only 147 (MS = 71; HS = 76) know that (by) brushing can re-mineralize hard dental structures. The duration of the toothbrushing is variable, for 2- or 3-min p = 0.058, r = 0.043. Criteria for choosing the toothbrush were based mainly on the indications of the dentist, respectively, for toothpaste on its properties. The frequency of toothbrushing is mainly twice a day 428 (MS = 234; HS = 248), p = 0.079, r = 0.037, 73 (MS = 33; HS = 40) after every meal. p = 0.099, r = 0.095. Mouthwash is used by 421 (MS = 199; HS = 222) p = 0.111, r = -0.048, and 228 (MS = 199; HS = 222) after each brushing. Dental floss is used by 240 (MS = 106; HS = 134), p = 0.031, r = -0.073 and only 74 (MS = 41; HS = 33) after each brushing. Conclusions: there are differences in the level of knowledge and attitudes regarding the determinants of oral hygiene depending on the level of education.

[SEM comparative analysis of the composite diacrylic resin-enamel interface in laser and bur prepared cavities]. / Studiu de analiza comparativa SEM a interfetei rasina diacrilica compozita-smalt in cavitati preparate mecanic si kinetic cu laser.

UNLABELLED: The aim of this study was to demonstrate that the size of the hybrid layer (HL) in the prepared enamel mechanic (M) and kinetic (K) with laser (WaterlaseMDBiolase), evaluated by scanning electron microscopy (SEM) restored with two types of resin composite is different. MATERIAL AND METHOD: The study was realized in vitro on a sample of 40 extracted human premolar and molars teeth for orthodontic or periodontal reasons. The teeth were divided at random into equal four groups (Gr). There has been Class I cavities with a depth of 2 mm (M) at high and low speed with cylindrical diamond No.1 and globular carbide and (K) peaks MZ6-5, 5W+20 Hz 30% water 60% air. The teeth were restored using 3M Schotchbond Etch, 3M Single Bond Dental Adhesive System: Gr. 1: (K)(n = 10)-3M Filtekflow; Gr.2 : (K)(n = 10)3M FiltekZ250; Gr.3: (M)(n = 10)3M Filtekflow; Gr.4: (M)(n = 10)3M FiltekZ250. The materials were placed in a single layer and photoactivate source LED SmartLite (Dentsply), termocycling 500 cycles (5 degrees-55 degrees), stored (48h), cut lengthwise (diamond), polished, conditioned (H3PO4-37% -5s) analyzed by SEM (JEOLJSM 6390), SPSS 13.00 statistically analyzed (ANOVA, p = 0.05). RESULTS: Analysis of the (HL) has highlighted differences between groups p = 0.05 average size (HL) being Gr.1;20.80 (+/- 0.72)microm, Gr.2;4.034 (+/- 0.0076)microm, Gr.3, 14.13 (+/- 0.63)microm, Gr.4, 1,43 (+/- 0.16)microm. Value size (HL) was influenced by the cavity preparation method for samples prepared by laser p = 0,005 in terms of size and in advantage of mechanical preparations in terms of quality (HL). Also the value of (HL) size was influenced by the type of material used in advance Gr.2 p = 0.0017. CONCLUSIONS: The sealing of cavity depends more on the creep of materials and less on the type of preparation. Permission was obtained from a institutional ethical committee of "Gr.T. Popa" University of Medicine and Pharmacy Iasi and that subjects gave written, informed consent).