RESUMO
A fluorescence "turn-on" sensor (HSS) for detection of H2S was developed on the basis of NO2-NH2 reduction. HSS showed a high affinity and sensitivity to H2S over other reducing reagents, particularly for biothiols. Also, the short responding time and high linear dependence between fluorescence enhancement and H2S concentration had HSS behave as a rapid sensor for quantitatively detection of H2S in the biological level.
Assuntos
Técnicas de Química Analítica/instrumentação , Corantes Fluorescentes/química , Sulfeto de Hidrogênio/análise , Naftalimidas/química , Sulfeto de Hidrogênio/química , OxirreduçãoRESUMO
A N-aryl-1,8-naphthalimide based sensor (ES-1) bearing a trimethylsilyl ether has been synthesized by a two-step reaction for quantitative detection of fluoride (F(-)). ES-1 exhibited monomer/excimer emissions at 410 and 524 nm respectively in CH2Cl2. In the presence of F(-), the desilylation of trimethylsilyl ether caused decay of the excimer emission as well as enhancement of the monomer emission to give a ratiometric signal. The fluoride-triggered desilylation showed a high reaction rate and high affinity to F(-) over nine other interfering anions. ES-1 provided a novel fluorescence assay based on excimer-monomer switch of N-aryl-1,8-naphthalimide to quantitatively measure F(-) with a detection limit of 0.133 ppm.
