RESUMO
Fungi play many roles in different ecosystems. The precise identification of fungi is important in different aspects. Historically, they were identified based on morphological characteristics, but technological advancements such as polymerase chain reaction (PCR) and DNA sequencing now enable more accurate identification and taxonomy, and higher-level classifications. However, some species, referred to as "dark taxa", lack distinct physical features that makes their identification challenging. High-throughput sequencing and metagenomics of environmental samples provide a solution to identifying new lineages of fungi. This paper discusses different approaches to taxonomy, including PCR amplification and sequencing of rDNA, multi-loci phylogenetic analyses, and the importance of various omics (large-scale molecular) techniques for understanding fungal applications. The use of proteomics, transcriptomics, metatranscriptomics, metabolomics, and interactomics provides a comprehensive understanding of fungi. These advanced technologies are critical for expanding the knowledge of the Kingdom of Fungi, including its impact on food safety and security, edible mushrooms foodomics, fungal secondary metabolites, mycotoxin-producing fungi, and biomedical and therapeutic applications, including antifungal drugs and drug resistance, and fungal omics data for novel drug development. The paper also highlights the importance of exploring fungi from extreme environments and understudied areas to identify novel lineages in the fungal dark taxa.
RESUMO
Mating disruption of Cadra cautella (Walk.) (Lepidoptera: Pyralidae) using its sex pheromone components, (Z, E)-9,12-tetradecadienyl acetate (ZETA) and (Z)-9-tetradecadien-1-yl acetate (ZTA), is successful in its population management. In addition, botanical oils have extensively been investigated in stored product pest management, but the effect of synthetic sex pheromones on the mating of C. cautella in the presence of plant volatiles is still unknown. Spinosad is used in food facilities as a contact insecticide but, if C. cautella larvae burrow into food, they may escape from spinosad. Importantly, the impact of spinosad on burrowing ability of C. cautella remains unknown. Therefore, the objectives of this study were to determine the effects of sex pheromone components ZETA and ZTA in the presence of botanical oils on the mating of C. cautella and the burrowing ability of C. cautella larvae in different types of flour treated with spinosad. In the first study, male and female moths were introduced into the cubicle having botanical oils and pheromone components. The mating status of female moths and male moth attraction to the trap was determined. The control experiments had only the botanical oils or pheromones. In the second study, burrowing ability of C. cautella larvae through different flour types was evaluated over 10 d. The flour was sprayed with spinosad (treatments) or water (controls). The mating success was higher with botanical oils alone but declined with exposure to pheromone either alone or combined with botanical oils. No differences in male attraction to traps were observed in botanical only, pheromone only or pheromone + botanical oil treatments. The burrowing of C. cautella larvae differed with flour type and spinosad altered burrowing ability. Thus, we conclude that the mating and burrowing of C. cautella is influenced by its pheromone and by exposure to botanicals and spinosad.
