RESUMO
A comparative study was designed to assess the bioequivalence of 2 oral poliovaccines (OPV) produced on 2 different cell systems: primary monkey kidney (PMK) cells and the Vero cell line. The Vero cell line has been used to overcome the problem of obtaining a regular supply of high quality monkeys that are devoid of latent viruses. For this study, 9 children were vaccinated with PMK-OPV and 12 children with Vero-OPV. The comparison covered poliovirus excretion, reversion of polioviruses in the 5'-noncoding region, and immunogenicity. Major molecular markers in the 5'-noncoding region related to neurovirulence already had been identified at position 480 for type 1, position 481 for type 2, and position 472 for type 3 poliovirus. Two nucleic-acid based methods were designed for studying these positions: a RT-PCR followed by sequencing, which required preliminary culture and cloning; and a type-specific nested PCR followed by sequencing, which enabled direct detection and genotyping of polioviruses. Twenty-eight stool specimens were analyzed by this second method with no PCR inhibition problem. The use of Vero cell line did not modify the global pattern of poliovirus excretion, reversion frequency, or seroconversion. These results provide additional support for the use of the well-characterized Vero cell line in OPV manufacturing.
Assuntos
Vacina Antipólio Oral/imunologia , Vacina Antipólio Oral/isolamento & purificação , Poliovirus/genética , Poliovirus/imunologia , Animais , Anticorpos Antivirais/sangue , Linhagem Celular , Chlorocebus aethiops , Clonagem Molecular , Fezes/química , Fezes/virologia , Genótipo , Humanos , Lactente , Macaca fascicularis , Macaca mulatta , Testes de Neutralização , Projetos Piloto , Poliomielite/genética , Poliomielite/prevenção & controle , Poliomielite/virologia , Poliovirus/química , Poliovirus/isolamento & purificação , Vacina Antipólio Oral/biossíntese , Reação em Cadeia da Polimerase , Sorotipagem , Vacinação/métodos , Vacinação/estatística & dados numéricos , Células Vero , Virulência , Eliminação de Partículas ViraisRESUMO
On day 0, four groups of children (3 to 6 months old) randomly received IPV alone or IPV + pertussis, or IPV + DP, or IPV + DTP. At days 28 and 56, all the children received the same IPV + DTP vaccine. Polio neutralizing and diphtheria antibodies were determined at days 0, 28 and 56. No adjuvant and even some inhibitory effect of pertussis was observed at days 28 and 56 on mean polio antibody titers. These results are compared to those observed with diphtheria.