RESUMO
Antibody titres to Enterobacterial common antigen (ECA) were assayed in 482 serum specimens from healthy adults and in 40 serum specimens from patients with acute pelvic inflammatory disease by indirect haemagglutination. Normal levels of anti-ECA ranged from 1:4 to 1:256. The mean serum titre was 1:64. The titres were found to be maximal in subjects between ages 31 and 40. The serum titres of females were slightly higher than the titres of males. Significant increases in the titres of anti-ECA were found in 11 of the 17 anti-ECA positive patients who had no concomitant gonococcal infection. ECA antibodies in this study were mostly IgM. The IgG class of antibody was found in 1.1 percent.
Assuntos
Anticorpos Antibacterianos/análise , Antígenos de Bactérias/imunologia , Doença Inflamatória Pélvica/imunologia , Adulto , Enterobacteriaceae/imunologia , Feminino , Gonorreia/complicações , Gonorreia/imunologia , Testes de Hemaglutinação , Humanos , Masculino , Pessoa de Meia-Idade , Doença Inflamatória Pélvica/complicaçõesRESUMO
An inhibitor to cell-bound HA was found to be produced at the non-haemagglutinating phase of the culture cycle by a classical vibrio strain which produced a cell-bound HA early and transiently during its growth. The HA-negative filtrate obtained from the late log-culture was found to inhibit the cell-bound HA activity produced by the same vibrio strain. It was also found to be produced early in shaking cultures at 37 degrees C and to mask the activity of early cell-bound HA in whole culture tests. This inhibitor is suggested to be responsible for the failure to obtain HA activity or adhesive vibrio cells grown at 37 degrees C and for the transient expression of cell-bound HA by some V. cholerae strains.
Assuntos
Proteínas de Bactérias/antagonistas & inibidores , Hemaglutininas/imunologia , Vibrio cholerae/imunologia , Proteínas de Bactérias/biossíntese , Proteínas de Bactérias/imunologia , Meios de Cultura , Testes de Inibição da Hemaglutinação , Vibrio cholerae/crescimento & desenvolvimentoRESUMO
The two biotypes of Vibrio cholerae were found to produce two distinct cell-bound haemagglutinins (HAs). El Tor vibrios, most of their nonmotile mutants and nonpathogenic El Tor strains produced a mannose-sensitive cell-bound HA constitutively under all growth conditions examined. Some classical vibrios, their nonmotile mutants and antigenically rough mutants of classical strains produced a fucose-sensitive cell-bound HA continually. Other classical vibrios produced neither cell-bound HA nor a fucose-sensitive cell-bound HA transiently.
