RESUMO
We studied the interaction of cytochrome c (cyt c) with specific calixarenes (CX) incorporated into the large unilamellar vesicles (LUV) composed of dimyristoylphosphatidylcholine (DMPC) or supported lipid membranes (sBLM) and compared this with not specific adsorption of cyt c to the LUV containing DMPC and anionic phosphatidic acid (PA) or sBLM composed of a mixture of DMPC and dimyristoylphosphatidic acid (DMPA). We showed that with increasing concentration of CX the average size of LUV increased and zeta potential become more negative as it is suggested from dynamic light scattering experiments. For PA containing LUV the increase in vesicle diameter was less expressed, but zeta potential decreased similarly like that of LUV contained CX. Cyt c did not affect significantly the LUV size, but reduced the negative zeta potential both for CX and PA containing vesicles. Electrochemical impedance spectroscopy allowed us to determine binding of cyt c to sBLM contained CX or DMPA. In both cases we observed decrease of charge transfer resistance with increasing cyt c concentration. The analysis of binding process suggests that the main driving force for interaction of cyt c with sBLM is the negative surface charge.
