RESUMO
OBJECTIVES: The aim of the study was to evaluate the expression of tumor necrosis factors -alpha and beta (TNF), their receptor and content in human uterine leiomyomas at various stages of tumor growth. MATERIAL AND METHODS: Studies were performed on human myometrium and uterine leiomyomas of various weights (small: less than 10 g and large: more than 100 g). Presence of both growth factors and their receptor was detected by Western Immunoblotting technique. The content of TNF-alpha was evaluated by immunoenzymatic method (ELISA). RESULTS: Changes in the expression of tumor necrosis factors and their receptor and difference in content of TNF-alpha during the tumor growth were found. CONCLUSIONS: Myometrium conversion into leiomyoma and an increase in its mass is accompanied by changes in the expression and contents TNF and TNF RI.
Assuntos
Leiomioma/metabolismo , Leiomioma/patologia , Linfotoxina-alfa/metabolismo , Fator de Necrose Tumoral alfa/metabolismo , Neoplasias Uterinas/metabolismo , Neoplasias Uterinas/patologia , Adulto , Biomarcadores Tumorais/metabolismo , Western Blotting , Proliferação de Células , Ensaio de Imunoadsorção Enzimática , Feminino , Fator 1 de Crescimento de Fibroblastos/metabolismo , Fator 2 de Crescimento de Fibroblastos/metabolismo , Humanos , Pessoa de Meia-Idade , Miométrio/metabolismo , Miométrio/patologia , Estadiamento de NeoplasiasRESUMO
OBJECTIVES: The aim of the study was the evaluation of acidic and basic FGF expression, as well as collagenolitic activity in human uterine leiomyomas at various stages of tumour growth. MATERIAL AND METHODS: Studies were performed on human myometrium and uterine leiomyomas of various weights (small: i.e. less than 10 g, and large: i.e. more than 100 g). The RT-PCR method was used to determine the acidic and basic FGF mRNA levels. The content of both FGF was evaluated by immunoenzymatic method (ELISA). The collagenolitic activity was detected by zymography. RESULTS: A distinct increase in the expression of aFGF, the amounts of both FGFs, and collagenolitic activity was observed during the tumour growth. CONCLUSIONS: Myometrium conversion into leiomyoma and an increase in its mass is accompanied by a significant increase in aFGF gene expression. The collagenolitic activity elevation favours a release of both FGF isoform from complexes with extracellular matrix components.
