[Amino acid losses in human sweat. Influence of oral contraceptives, physical exercise and sex on the excretion of amino acids in sweat (author's transl)]. / Aminosäurenverluste im menschlichen Schweiss. Einfluss von Hormoneinnahme, körperlichem Training und vom Geschlecht auf die Aminosäurenausscheidung im Schweiss.

UNLABELLED: With the aid of ion exchange column chromatography we determined quantitatively the free amino acids in thermal sweat of the total human body surface. Sweat of 60 healthy men and 63 healthy women (aged 18-31 years) collected during 12 min of sauna bathing was investigated. Thirty men and 31 women were well trained, 33 women were taking oral, hormonal contraceptives. Sweat was deproteinised by adding an equal volume of 5% sulphosalicylic acid. RESULTS: 1. The comparison of the women taking oral contraceptives with the women not taking oral contraceptives showed no qualitative or significant quantitative differences in the excretion of amino acids in thermal sweat collected from the total body surface. 2. The comparison of the trained women with the untrained women neither revealed qualitative or significant quantitative differences in the excretion of amino acids in thermal sweat collected from the total body surface. 3. As compared to the trained men, untrained men excreted in thermal sweat collected from the total body surface significantly more alanine, arginine, citrulline, cystine, glycine, histidine, isoleucine, leucine, lysine, phenylalanine, serine, threonine, tyrosine, and valine. 4. The sex-specific comparison of the amino acid excretion in sweat showed that men had a significantly increased excretion of cystine, histidine, leucine, and taurine. Proline was detected only in the sweat of women. 5. The quantitative results obtained for the amino acid excretions during 12 min of sauna bathing suggest that persons exposed to hard, physical activity and/or high environmental temperatures have quite equal amino acid losses in sweat and in 24 h urine.

[Study of the glucose concentration in five vascular regions -- aorta, arteria pulmonalis, vena cava inferior, vena cava superior, vena hepatica -- in childhood (author's transl)]. / Verhalten der Glucose-Konzentration in fünf Gefässgebieten -- Aorta, Arteria pulmonalis, Vena cava inferior, Vena cava superior, Vena hepatica -- des kindlichen Organismus.

Using the Beckman glucose analyzer, glucose concentration was determined in the serum of the fasting blood in five vascular regions -- aorta, arteria pulmonalis, vena cava inferior, vena cava superior, vena hepatica -- of 52 children (ages 2,5 months up to 14 years and 3 months, x = 6 years and 3 months, male = 28, female = 24) with a healthy metabolism. The comparison of the average values of the glucose concentrations determined in five vascular regions in childhood showed that the glucose concentration in the vena hepatica was statistically significant higher than in the other vascular regions. Moreover the comparison of the glucose concentrations revealed that the values in the vena cava inferior were statistically significant higher as compared to the values in the vena cava superior. The glucose concentrations in the arteria pulmonalis, the vena cava superior and the aorta were nearly equal. Compared with the older children, the younger children had in all the investigated vascular regions lower glucose concentrations.

Quantitative study of free amino acids in human eccrine sweat excreted from the forearms of healthy trained and untrained men during exercise.

The free amino acids in eccrine sweat collected from the forearms of 20 healthy trained and 20 healthy untrained men during controlled exercise were determined quantitatively using ion exchange column chromatography. Sweat was deproteinized by adding an equal volume of 5% sulphosalicylic acid. The amino acid concentrations showed a constant qualitative pattern in sweat and large individual differences. Essential amino acids, such as isoleucine, leucine, lysine, methionine, phenylalanine, and valine were excreted in relatively small amounts. As compared to the trained men, untrained men showed statistically significantly higher concentrations in sweat for the following amino acids: Alanine, arginine, glycine, histidine, isoleucine, leucine, lysine, ornithine, phenylalanine, serine, taurine, threonine, tyrosine, and valine. No significant differences were found for citrulline, cystine, ethanolamine, and methionine. The comparison of the amino acid excretions in sweat obtained under controlled exercise and in urine showed that the amounts of amino acids excreted in sweat under controlled exercise were comparable to the losses of amino acids in urine.

[Normal range of free amino acids in cerebrospinal fluid of children (author's transl)]. / Verhalten der freien Aminosäuren im Liquor cerebrospinalis von Kindern

UNLABELLED: Free amino acids were determined quantitatively in cerebrospinal fluid of 80 boys and 69 girls (ages 1 month-14years). On the basis of the cliniclal criterions these children were considered to be ""healthy''. The total number of examined children was 2000. Cerebrospinal fluid was mixed with an equal volume of 5% sulphosalicylic acid and the amino acids were separated by ion exchange column chromatography. RESULTS: 1. The sexspecific investigation of the 1 month -2 years old children showed that the boys had a statistical significant hgher concentration of lysine and that no statistical significant differences existed between the groups of 2-14 years old children. 2. The age specific comparison revealed that the amino acid concentrations ofaspartic acid, citrulline, histidine, lysine, serine, threonine and tyrosine were in the cerebrospinal fluid of the younger boys statistical significant higher as compared to the older boys and that the concentration of serine in the cerebrospinal fluid of the younger girls was statistical significant higher than in the group of older girls.

[Effect of urethane (DAB-6 product), hexobarbital-Na and pentobarbital-Na on the amino acid concentrations in blood serum of rats. I. Communication (author's transl)]. / Verhalten der freien Aminosäuren im Serum von Ratten vor und nach Behandlung mit Urethan, Hexobarbital-Na und pentobarbital-Na. I. Mitteilung

UNLABELLED: Free amino acids were determined quantitatively in serum from the fasting blood of rats before and after treatment with urethane (n = 20), hexobarbital-Na (n = 20) and pentobarbital-Na (n = 20). Serum was deproteinised by adding an equal volume of 5% sulphosalicyclic acid and the amino acids were separated by ion exchange column chromatography. RESULTS: 1.24 h after treatment with urethane the rats showed only a statistically significant higher concentration of ornithine in serum. - 2.24 h after injection of hexobarbital-Na the rats had statistically significant higher concentrations of arginine, isoleucine, leucine, lysine, methionine, phenylalanine, taurine, tryptophan and tyrosine in serum - 3.24 h after application of pentobarbital-Na the rats showed statistically significant higher concentrations of aspartic acid, glutamic acid, histidine, lysine, phenylalanine, serine, taurine, threonine, and tyrosine and statistically significant lower concentrations of argine and citrulline in serum.