Application of distant live broadcast in clinical anesthesiology teaching.

OBJECTIVE: To explore the application value and effect of distance live broadcast in Clinical Anesthesiology teaching. METHODS: Undergraduate students of year 2017 who majored in Anesthesiology at the Wannan Medical College (China) were chosen as the study subjects. According to the different teaching methods, the students were divided into two groups: 59 in the traditional teaching group (control group) and 61 in the traditional teaching combined with distant live broadcasting teaching group (observation group). The teaching feedback, students' satisfaction, and the theory and skill scores of the course were compared between the two groups. RESULTS: The teaching feedback in the observation group was better than that in the control group (P<0.05). The students' satisfaction rate with teaching and the theory and skill learning score in the observation group were higher than those of the control group (P<0.05). CONCLUSION: Traditional teaching combined with distant live broadcast teaching has achieved good results in clinical anesthesiology teaching, improved the overall quality of teaching, and has high clinical teaching value.

Research progress of ulinastatin in the treatment of liver diseases.

Ulinastatin (UTI) is a trypsin inhibitor observed in urine. UTI can treat some diseases by inhibiting the broad-spectrum hydrolysis activity of various enzymes and other pharmacological effects. UTI can widely treat pancreatitis, systemic multiple organ dysfunction syndrome, circulatory failure, and toxic shock clinically. The liver is a major metabolic organ of the human body. Various biological metabolic reactions require the liver's participation. When various physical and chemical factors drive the body, it will damage the liver to varying degrees. As a clinically effective drug, UTI is also known to treat some liver diseases. This article mainly describes UTI's research progress in treating septic liver injury, hepatitis, liver fibrosis, autoimmune liver disease with liver failure, and liver ischemia-reperfusion injury.

[Application of micellar electrokinetic capillary chromatography for identification, quantitative detection and unfolding analysis of interleukin-12].

OBJECTIVE: To establish a micellar electrokinetic capillary chromatography-based method for identification and quantitative detection of interleukin-12 (IL-12) and analysis of its unfolding process. METHODS: An uncoated fused-silica capillary (inner diameter 50 µm) with a total length of 48.5 cm (40 cm to the detector) was used for the experiment. The factors influencing the separation efficiency of IL-12 were analyzed, and a standard curve of IL-12 concentration was established. The mixture of IL-12 and anti-IL-12 antibody was incubated in a water bath at 38 ℃ for 40 min, and capillary electrophoresis was then performed under the same conditions. The results were compared with those of IL-12 and anti-IL-12 antibody to identify IL-12. IL-12 and dithiothreitol (DTT) were incubated at 60 ℃ in water bath for different lengths of times, and the unfolding process of IL-12 was analyzed based on electrophoresis results of IL-12 in different states. RESULTS: A micellar capillary electrophoresis on-line sweep method was established with 80 mmol/L borate (pH=9.3) containing 30 mmol/L sodium dodecyl sulfate (SDS) as the buffer solution. This system showed a good linear relationship between the peak area and the mass concentration of IL-12 with a linear correlation coefficient of 0.9991 within the linear range of 2 to 120 ng/L. As the incubation time of IL-12 and DTT prolonged, the disulfide bond of IL-12 gradually opened and resulted in distinct changes in the protein peak. CONCLUSIONS: This capillary electrophoresis-based method is simple and sensitive for IL-2 analysis and allows rapid detection of changes in IL-12 content in the setting of tumors and analysis of the possible causes.

Purification and characterization of Protein C activator from Agkistrodon acutus Venom.

Protein C (PC) plays an important role in the balance of coagulation and anticoagulation. Thus, the detection of PC activity is diagnostically significant for patients with cardiovascular diseases. Presently, the key methods to detect PC activity are the chromogenic assay and activated partial thromboplastin time (APTT) test. PROTAC used in the chromogenic assay is isolated from Agkistrodon contortrix venom as protein C activator (PCA). However, the use of the chromogenic assay is limited because of the high price of PROTAC. In this study, PCA was successfully purified from Agkistrodon acutus venom (AAV) by ion-exchange and gel chromatography. PCA from AAV has a relative molecular mass of 24 kD, calculated from the measurement of 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The components of PCA were identified by MALDI-TOF/TOF-MS and mascot searches revealed that the coverage rate between PCA and zinc metalloproteinase AaPA from AAV was 21%. The chromogenic assay and APTT test were used to measure the enzymatic activity of PCA, and the results showed that PCA from AAV could specifically activate PC. In summary, the chromogenic assay described herein is highly sensitive and easy to perform.

[Application of capillary zone electrophoresis in the interaction analysis of protein C with protein C activator from Agkistrodon acutus venom].

A new capillary zone electrophoresis method (CZE) has been established for the interaction analysis of protein C (PC) with a protein C activator (PCA) from Agkistrodon acutus venom. The analysis was performed on an uncoated fused-silica capillary with 75 microm i.d. and a total length of 60.2 cm (50 cm to the detector) with a buffer solution of 50 mmol/L Tris-HCl (pH 7.4) and 198 nm of wavelength. The factors which influence the separation of the PCA, such as buffer solution and ion concentration, and the interaction between the PCA and PC incubated for different times at 37.5 degrees C were studied. The linear range was from 10 to 300 mg/L. The limit of detection was 3 mg/L (S/N = 3). The relative standard deviation (RSD) for the migration time of the PCA was 0.56%. The RSD for the peak area was 3.8% (n = 6). The equal volumes of the PCA (200 mg/L) and PC (60 mg/L) were incubated for five minutes, at which their binding rate reached the maximum. And no hydrolyzed peptide chain from PC was found in the electropherogram. The PCA from Agkistrodon acutus venom could activate PC directly through changing the space conformation of PC. The method is simple, and highly sensitive with high resolution, and will provide important theoretical basis for the rapid detection of venom proteins and their activities in the future.

Luteolin ameliorates cardiac failure in type I diabetic cardiomyopathy.

OBJECTIVE: The study aimed to determine whether luteolin can confer cardioprotective effects against diabetic cardiomyopathy in relation to specific and quantitative markers of oxidative stress. METHODS: We examined diabetic cardiomyopathy by left ventricular hemodynamic analysis. Myocardial oxidative stress was assessed by measuring the activity of superoxide dismutase (SOD) as well as the level of malondialdehyde (MDA). Hypolipidaemic effects of luteolin were also investigated in STZ-induced diabetic rats. Myocardial Akt/PKB phosphorylation, heme oxygenase-1 (HO-1) and connective tissue growth factor (CTGF) protein levels were measured by Western blot in all rats at the end of the study. RESULTS: This study showed a significant increase in serum triacylglycerol (TG), total cholesterol (TC), lower density lipoprotein (LDL), MDA content, creatine kinase (CK), lactate dehydrogenase (LDH), and myocardial CTGF and a significant decrease in high density lipoprotein (HDL), SOD and Akt phosphorylation level in the diabetic group compared to the control group. Luteolin treatment induced a significant decrease in serum TG, TC, LDL, MDA, CK, LDH, and myocardial CTGF and a significant increase in HDL, SOD and Akt phosphorylation levels in comparison with the diabetic group. CONCLUSION: These results show that luteolin protects against the progression of diabetes mellitus-induced cardiac dysfunction by attenuation of myocardial oxidative stress probably through its antioxidant properties.