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Gene ; 565(2): 228-34, 2015 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-25865296

RESUMO

Mammary glands are organs for milk production in female mammals. Growth hormone (GH) is known to affect the growth and development of the mammary gland, as well as to increase milk production in dairy goats. This study performed a comprehensive expression profiling of genes expressed in the mammary gland of early involution GH transgenic (n=4) and non-transgenic goats (n=4) by RNA sequencing. RNA was extracted from mammary gland tissues collected at day 3 of involution. Gene expression analysis was conducted by Illumina RNA sequencing and sequence reads were assembled and analyzed using TopHat. FPKM (fragments per kilobase of exon per million) values were analyzed for differentially expressed genes using the Cufflinks package. Gene ontology analysis of differentially expressed genes was categorized using agriGO, while KEGG pathway analysis was performed with the online KEGG automatic annotation server. Our results revealed that 75% of NCBI goat annotated genes were expressed during early involution. A total of 18,323 genes were expressed during early involution in GH transgenic goats, compared with 18,196 expressed genes during early involution of non-transgenic goats. In these expressed genes, the majority (17,589) were ubiquitously expressed in GH transgenic and non-transgenic goats. However, there were 745 differentially expressed genes, 421 of which were upregulated and 324 were downregulated in GH transgenic goats. GO and KEGG pathway analysis showed that these genes were involved in mammary gland physiology, including cell adhesion molecules, ECM-receptor interaction, Jak-STAT signaling pathway, and fat metabolism. Our results demonstrated that the GH receptor was strongly affected in GH transgenic goats, which may activate the IGF-1/Stat3 signaling pathway. Overall, our study provided a global view of the transcriptome during involution of GH transgenic and non-transgenic goats, which increases our understanding of the biology of involution in the goat.


Assuntos
Cabras/genética , Hormônio do Crescimento/genética , Glândulas Mamárias Animais/metabolismo , Transcriptoma/genética , Animais , Animais Geneticamente Modificados/genética , Regulação para Baixo/genética , Feminino , Expressão Gênica/genética , Perfilação da Expressão Gênica/métodos , Fator de Crescimento Insulin-Like I/genética , Janus Quinases/genética , Lactação/genética , Leite/metabolismo , RNA/genética , Receptores da Somatotropina/genética , Fator de Transcrição STAT3/genética , Análise de Sequência de RNA/métodos , Transdução de Sinais/genética , Regulação para Cima/genética
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