RESUMO
The distribution of bancol in the organism of the warm-blooded animals was studied following a single intragastric administration of this poisonous agent to the rats in the form of aqueous suspension at a dose equivalent to double LD50. It was shown that the largest amounts of bancol were accumulated in the stomach contents, heart, and spleen.
Assuntos
Benzenossulfonatos , Intoxicação , Animais , Benzenossulfonatos/química , Benzenossulfonatos/farmacocinética , Benzenossulfonatos/toxicidade , Inibidores da Colinesterase/química , Inibidores da Colinesterase/farmacocinética , Inibidores da Colinesterase/toxicidade , Inseticidas/química , Inseticidas/farmacocinética , Inseticidas/toxicidade , Masculino , Modelos Animais , Intoxicação/etiologia , Intoxicação/patologia , Ratos , Espectrofotometria Ultravioleta/métodos , Distribuição TecidualRESUMO
Optimal conditions for the extraction of tetramethyltiuram disulfide (TMTD) from biological materials with ethylacetate are described. The isolated compound can be separated from co-extracted impurities on a silicagel L (40/100 mcm) column. Identification and quantitative determination of TMTD purified from cadaverous liver were performed using thin-layer chromatography, IR spectrophotometry, and high performance liquid chromatography with UF detection.
Assuntos
Acetatos/química , Medicina Legal/métodos , Fungicidas Industriais/análise , Fígado/química , Tiram/análise , Adulto , Cadáver , Cromatografia Líquida de Alta Pressão/métodos , Cromatografia em Camada Fina/métodos , Humanos , Masculino , Sensibilidade e Especificidade , Espectrofotometria Infravermelho/métodosRESUMO
Optimal conditions for the extraction of bancol from the biological material with toluene are described. The possibility of its purification and separation from co-extracted compounds on a silicagel L column, 40/100 mcm is illustrated. Identification and quantitative determination of bancol isolated from the cadaverous liver were performed by the electron spectrophotometry technique, thin-layer chromatography, and high performance liquid chromatography using normal-phase sorbents. A method of bancol detection was adapted for the purpose of forensic medical expertise and applied for the postmortem examination of the cadaverous tissues.
Assuntos
Benzenossulfonatos/análise , Toxicologia Forense/métodos , Fígado/química , Espectroscopia Fotoeletrônica/métodos , Benzenossulfonatos/química , Cromatografia Líquida de Alta Pressão/métodos , Cromatografia em Camada Fina/métodos , Humanos , Tolueno/químicaAssuntos
Antígenos HLA/análise , Hanseníase/imunologia , Saúde da Família , Antígenos HLA/genética , Antígenos HLA/imunologia , Haplótipos , Humanos , Hanseníase/genética , Hanseníase Virchowiana/genética , Hanseníase Virchowiana/imunologia , Hanseníase Tuberculoide/genética , Hanseníase Tuberculoide/imunologiaRESUMO
The "plasmid rescue" method has been used to isolate the recombinant plasmid pMA3 fragment and flanking sequences from the transgenic mouse genome containing the fragment in integrated form. The "rescued" plasmid, pMAR1, lacks all virus sequences and retains only those regions of pBR322 that are responsible for the plasmid replication and Escherichia coli ampicillin resistance. The plasmid pMA3 deletion has occurred at its integration into the mouse genome after microinjection into the zygote. The integrated fragment of the plasmid is adjacent to the genome repeated sequence that is highly conservative in evolution.
