RESUMO
'Protein displays of the Human Immunoglobulin Heavy, Kappa and Lambda Variable and Joining Regions', the 6th report of the 'IMGT Locus on Focus' section, comprises 4 figures: (1) 'Protein display of human IGH V-REGIONs'; (2) 'Protein display of human IGK V-REGIONs'; (3) 'Protein display of human IGL V-REGIONs and V-PREB REGION'; (4) 'Protein display of human IGH, IGK and IGL J-REGIONs', and 1 table entitled: 'FR-IMGT and CDR-IMGT length of the human IGHV, IGKV, IGLV and V-PREB genes'. These figures and table are available at the IMGT Marie-Paule page from IMGT, the international ImMunoGeneTics database (http://imgt.cnusc.fr: 8104) created by Marie-Paule Lefranc, Université Montpellier II, CNRS, France.
Assuntos
Genes de Imunoglobulinas/genética , Região de Junção de Imunoglobulinas/genética , Cadeias kappa de Imunoglobulina/genética , Cadeias lambda de Imunoglobulina/genética , Sequência de Aminoácidos , Humanos , Dados de Sequência MolecularRESUMO
IMGT, the international ImMunoGeneTics database (http://imgt.cnusc. fr:8104), is a high-quality integrated database specialising in Immunoglobulins (Ig), T cell Receptors (TcR) and Major Histocompatibility Complex (MHC) molecules of all vertebrate species, created in 1989 by Marie-Paule Lefranc, Université Montpellier II, CNRS, Montpellier, France (lefranc@ligm.igh.cnrs.fr). IMGT comprises three databases: LIGM-DB, a comprehensive database of Ig and TcR, MHC/HLA-DB, and PRIMER-DB (the last two in development); a tool, IMGT/DNAPLOT, developed for sequence analysis and alignments; and expertised data based on the IMGT scientific chart, the IMGT repertoire. By its high quality and its easy data distribution, IMGT has important implications in medical research (repertoire in autoimmune diseases, AIDS, leukemias, lymphomas), therapeutic approaches (antibody engineering), genome diversity and genome evolution studies. IMGT is freely available at http://imgt.cnusc. fr:8104
Assuntos
Bases de Dados Factuais , Genes de Imunoglobulinas , Genes Codificadores dos Receptores de Linfócitos T , Imunogenética , Complexo Principal de Histocompatibilidade , Animais , França , Variação Genética , Imunoglobulinas/química , Imunoglobulinas/genética , Armazenamento e Recuperação da Informação , Internet , Complexo Principal de Histocompatibilidade/genética , Modelos Moleculares , Receptores de Antígenos de Linfócitos T/química , Receptores de Antígenos de Linfócitos T/genética , Análise de Sequência , Terminologia como Assunto , VertebradosRESUMO
'Human Immunoglobulin Kappa Variable (IGKV) Genes and Joining (IGKJ) Segments', second report of the 'IMGT Locus on Focus' section, comprises five tables entitled: (1) 'Number of human germline IGKV genes at 2p12 and potential repertoire'; (2) 'Human germline IGKV gene table'; (3) 'Human IGKV allele table'; (4) 'Human germline IGKJ table' and (5) 'Human IGKJ allele table'. These tables are available at the IMGT Marie-Paule page from IMGT, the international ImMunoGeneTics database (http://imgt.cnusc.fr:8104) created by Marie-Paule Lefranc, CNRS, Université Montpellier II, Montpellier, France.
Assuntos
Cadeias J de Imunoglobulina/genética , Cadeias kappa de Imunoglobulina/genética , Bases de Dados como Assunto , Genes , Humanos , Fases de Leitura Aberta , PseudogenesRESUMO
In response to the growing interest in the prosimian Microcebus murinus for studies on cerebral aging, the stereotaxic atlas of its brain was carried out in view of further anatomical, biochemical, electrophysiological, and behavioral investigations as well as for therapeutic experiments. This primate, which could be a valuable model for neuroscientific studies in various domains, presents numerous physiological advantages (e.g., size, cost, and ability to breed) compared to rodents, which can be used as nonprimate models, and simians. The atlas, valid for adult microcebes of every age and both sexes, consists of 54 frontal plates and 28 sagittal plates. For the establishment of stereotaxic coordinates and for drawings and photographs, 10 adult specimens of Microcebus murinus were used. The brains were frozen, cut into sections of 50 microm thickness, every fourth section being stained with Nissl. First, sections were projected and the outlines of the different structures, nuclei, and fibers were drawn. Then, the accuracy of the analysis was improved by detailed observation directly by microscope and also by computer analysis. Finally, the photographs of the sections were scanned and processed using the software Photoshop and Illustrator. For testing coordinates, several verifications were made. Experiments on lesions and injections of different substances were carried out in specific regions of the brain and brains implanted with needles were fixed in formol and embedded in paraffin wax.
