Validation of stability-indicating LC method, degradation study, and impact on antioxidant and antifungal activities of Eugenia uniflora leaves extract.

The aim of this study is to demonstrate the stability-indicating capacity of an analytical method for Eugenia uniflora, enhance understanding of the stability of myricitrin, and assess the effect of degradation of spray-dried extract (SDE) on antioxidant and antifungal activities. Validation of the stability-indicating method was carried out through a forced degradation study of SDE and standard myricitrin. The antioxidant and antifungal activities of SDE were evaluated both before and after degradation. The quantification method described was found to be both accurate and precise in measuring myricitrin levels in SDE from E. uniflora, with excellent selectivity that confirmed its stability-indicating capability. The forced degradation study revealed that the marker myricitrin is sensitive to hydrolysis, but generally stable under other stress conditions. By contrast, the standard myricitrin displayed greater susceptibility to degradation under forced degradation conditions. Analysis of the antioxidant activity of SDE before and after degradation showed a negative impact in this activity due to degradation, while no significant effect was observed on antifungal activity. The method described can be a valuable tool in the quality control of E. uniflora, and the findings can assist in determining the optimal conditions and storage of products derived from this species.

Preparación preoperatoria: la percepción de la persona que se somete a una cirugía programada / Preoperative preparation: perceptions of elective surgery patients

Objetivo principal: Conocer la percepción de la persona sometida a una cirugía general programada sobre su preparación preoperatoria. Método: Estudio cualitativo y exploratorio. Se realizaron diez entrevistas semiestructuradas con personas que se habían sometido a una cirugía general programada en los últimos seis meses en un hospital universitario de la región central de Portugal. Para analizar los datos se utilizó la técnica del análisis de contenido. Resultados principales: Surgieron cinco unidades temáticas, percepción de la experiencia quirúrgica, sentimientos/emociones percibidas, necesidades de información, expectativas sobre los cuidados de enfermería preoperatorios y perspectivas sobre la existencia de una consulta de enfermería, que se subdividieron en varias categorías y subcategorías. Conclusión principal: Algunas personas siguen viendo la experiencia quirúrgica como algo traumático y consideran el entorno quirúrgico como un generador de estrés. Este estudio refuerza la relevancia del personal de enfermería en la preparación de las personas en situaciones perioperatorias y puede proporcionar importantes contribuciones a la estructuración de una consulta de enfermería. (AU)

Objective: This study aimed to identify elective surgery patients' perceptions of their preoperative preparation. Methods: A qualitative, exploratory study was carried out. Ten semi-structured audio-recorded interviews were conducted with people who had undergone elective general surgery in the last six months at a university hospital in central Portugal. The content analysis technique was used to analyze the data. Results: Five thematic units emerged: perception of the surgical experience, perceived feelings/emotions, information needs, expectations towards preoperative nursing care, and perspectives on the existence of a nursing consultation. They were subdivided into several categories and subcategories. Conclusions: Surgery is still considered a traumatic experience, and the surgical environment causes stress. This study reinforces the relevance of nurses in the preoperative preparation of patients and can contribute considerably to structuring a nursing consultation. (AU)

Confirming sulfluramid (EtFOSA) application as a precursor of perfluorooctanesulfonic acid (PFOS) in Brazilian agricultural soils.

Perfluorooctanesulfonic acid (PFOS) is a manmade chemical with several industrial applications and also a potential byproduct of many other per- and polyfluorinated substances (PFAS) in the environment. Due to the gathered evidence on its environmental persistence, long-range transport, toxicity, and bioaccumulative and biomagnifying properties, PFOS, its salts and perfluorooctane sulfonyl fluoride (PFOSF), were listed for global restriction under the Stockholm Convention on Persistent Organic Pollutants in 2009. Nevertheless, Brazil has granted an acceptable purpose exemption for using PFOSF to produce sulfluramid (EtFOSA) and to apply it as insecticide to control leaf-cutting ants of the genus Atta and Acromyrmex. Previous studies have pointed out EtFOSA as a precursor of PFOS in the environment, including in soils. Therefore, we aimed to confirm the role of EtFOSA in PFOS formation in soils representing areas where sulfluramid-based ant baits are used. A biodegradation assay was carried out by applying technical EtFOSA in triplicate samples of ultisol (PV) and oxisol (LVd) and measuring the contents of EtFOSA, perfluorooctane sulfonamide acetic acid (FOSAA), perfluorooctane sulfonamide (FOSA), and PFOS at seven moments (0, 3, 7, 15, 30, 60, and 120 days). The monitored byproducts started being noticed on the 15th day. After 120 days, PFOS yields were 30% for both soils, whereas FOSA yields were 46% (PV soil) and 42% (LVd soil) and FOSAA yields were 6% (PV soil) and 3% (LVd soil). It can be expected that FOSAA and FOSA contents will eventually be converted into PFOS in the environment and that the presence of plants could boost PFOS formation. Therefore, the ongoing extensive and intensive use of sulfluramid-based ant baits pose a considerable source of PFOS to the environment.

Evaluation of the Antioxidant and Antifungal Actions of the Optimized Crude Extract and Fractions from the Aerial Parts of Acanthospermum hispidum.

This study investigated the phytochemical characteristics of the aerial parts of Acanthospermum hispidum, by chromatographic and spectrophotometric methods, and evaluated the antioxidant and antifungal activities of the crude extract and polyphenol-enriched fractions of the species. The phytochemical prospection showed the presence of polyphenols from the groups of hydroxycinnamic derivatives and flavonoids in the crude extract (CE) and fractions of the aerial parts of A. hispidum. In the chromatographic analysis, it was possible to observe that the fractionation process of the CE with hexane and ethyl acetate was efficient in enriching the fractions in phenolic compounds. This enrichment provided an increase in antioxidant activity by the DPPH and ABTS methods, in which it was observed a higher antioxidant activity for EAF in the DPPH test and higher activity against the ABTS radical by the fractions AqF and RAqF. The extract and fractions were effective against Candida non-Candida albicans strains, mainly against C. glabrata, C. parapsilosis and C. krusei, acting predominantly fungicidal. The results indicate that the aerial parts of A. hispidum can serve as a basis for the development of new antioxidant and antifungal products. Moreover, the fractionation process can contribute to increasing the biological potential of the species.

Autopercepção do perfil facial de adolescentes e fatores associados: características do indivíduo, bem-estar e autoestima / Self-perception of the facial profile in adolescents and associated factors: individual characteristics, well-being and self-esteem

O período da adolescência é relevante no tratamento ortodôntico, pois nessa fase de desenvolvimento dentofacial ocorrem alterações que modificam demasiadamente a dentição e a face. Geralmente é nessa faixa etária que se inicia o tratamento ortodôntico e sua busca é desencadeada predominantemente por uma demanda estética. Durante o tratamento ortodôntico, o perfil facial pode sofrer grandes alterações. Sendo assim, o planejamento do tratamento ortodôntico baseado em evidência cientifica requer também a participação do paciente no relato da autopercepção sobre seu perfil facial. A autopercepção de pacientes no ciclo de vida da adolescência pode ser influenciada por fatores como a idade, sexo e autoestima. Diante disso, o objetivo desse estudo foi avaliar a influência da autoestima, do bemestar e das características do indivíduo na autopercepção do perfil facial em adolescentes. Foi realizado um estudo observacional transversal com uma amostra de 158 adolescentes de ambos os sexos, com idade entre 10 a 19 anos, pacientes e alunos de Graduação da Faculdade de Odontologia da Universidade Federal de Minas Gerais. A autopercepção dos perfis faciais foi avaliada pela apresentação de um template contendo 10 fotografias de perfil facial, sendo nove fotografias padronizadas e uma fotografia de perfil do participante do estudo. O participante foi solicitado a identificar o seu perfil facial dentre os 10 apresentados. As fotografias de perfil facial foram realizadas por uma pesquisadora treinada. A autoestima dos adolescentes foi medida por meio da versão brasileira validada da Escala de Rosenberg. No caso de adolescentes menores de 18 anos, seus pais/responsáveis responderam a um questionário sobre as condições socioeconômicas e demográficas de suas famílias, e sobre o quanto o bem-estar do adolescente era afetado pelas condições bucais. Os adolescentes de 18 anos ou mais responderam à mesmas questões. Análises descritivas e regressão de Poisson com variância robusta, não ajustada e ajustada, foram realizadas (p<0,05). O modelo de análise ajustada demonstrou que a autopercepção do perfil facial era maior em adolescentes mais velhos (Razão de Prevalência [RP]=1,79; 95%Intervalo de Confiança [IC]:1,34-2,40; p<0,001) e cujo bem-estar geral não era afetado pelas condições bucais (RP=1,41; 95%IC:1,10-1,81; p=0,007). Os adolescentes que relatam se sentirem fracassados (RP=0,63; 95%IC:0,46-0,85; p=0,003) e aqueles que apresentam uma atitude positiva consigo mesmo (RP=1,46; 95%IC: 1,00-2,14; p=0,050) têm maior probabilidade de reconhecer o seu perfil facial do que aqueles que não possuem esses sentimentos. Conclui-se que adolescentes mais velhos, que não tiveram seu bem-estar geral afetado pela condição bucal, que relataram sentirem-se fracassados e possuírem uma atitude positiva sobre si mesmos apresentaram maior probabilidade de reconhecer o seu perfil facial.

The period of adolescence is relevant in orthodontic treatment, as changes occur in this phase of dentofacial development that greatly modify the dentition and the face. It is usually in this age group that orthodontic treatment begins and its search is predominantly triggered by an aesthetic demand. Facial profile can undergo major changes during orthodontic treatment, according to the type of treatment. Therefore, orthodontic treatment planning based on scientific evidence also requires the patient's participation in reporting self-perception of their facial profile. The self-perception of patients in the adolescence life cycle can be influenced by factors such as age, sex and self-esteem. Therefore, the objective of this study was to evaluate the influence of self-esteem, well-being and individual characteristics on the self-perception of the oral facial profile in adolescents. A cross-sectional observational study was carried out with a sample of 158 adolescents of both sexes, aged between 10 and 19 years, patients and undergraduate students of the Faculty of Dentistry of Universidade Federal de Minas Gerais. The self-perception of facial profiles was assessed by presenting a template containing 10 profile photographs, nine of which were standardized photographs and one profile photograph of the study participant. The participant was asked to identify his/her soft tissue profile among the 10 photographs. Soft tissue profile photographs were taken by a trained researcher. The adolescents' self-esteem was measured using the validated Brazilian version of the Rosenberg Scale. For adolescents under 18 years of age, their parents/guardians completed a questionnaire about the socioeconomic and demographic conditions of the adolescents and their families, and about how much the adolescent's well-being was affected by oral conditions. Adolescents aged 18 years and over answered the same questions. Descriptive statistics and unadjusted and adjusted Poisson regression with robust variance were used for data analysis (p<0.05). The adjusted analysis model showed that self-perception of the facial profile was higher in older adolescents (PR=1.79; 95%CI:1.34-2.40; p<0.001) and whose general well-being was not affected by oral conditions (PR=1.41; 95%CI:0.91-1.81; p=0.007). Adolescents who report feeling like failures (PR=0.63; 95%CI:0.46-0.85; p=0.003) and those who have a positive attitude towards themselves (PR=1.46; 95% CI: 1.00-2.14; p=0.050) are more likely to recognize their facial profile than those who do not have these feelings. It is concluded that older adolescents, who did not have their general well-being affected by their oral condition, who reported feeling like failures and having a positive attitude about themselves, were more likely to recognize their facial profile.

Modeling Self-Sealing Mechanisms in Fractured Carbonates Induced by CO2 Injection in Saline Aquifers.

In the future, there will be competition among natural gas, CO2, and hydrogen for suitable geological storage sites. Therefore, it is crucial to evaluate all of the potential storage options. One promising option is the utilization of fractured carbonate rocks, which offer significant opportunities for gas sequestration in depleted reservoirs and saline aquifers. The objective of this study is to assess the feasibility of using carbonate matrix rocks in saline aquifers for carbon capture and storage (CCS) projects. Although carbonate reservoirs are currently not ranked highly for carbon storage due to the risk of CO2 leakage, their matrix rock with reactive minerals like calcite and dolomite, along with the possibility of natural fractures, presents an interesting opportunity. This research proposes a reassessment of the role of fractures, which are typically viewed as a risk factor, within a novel and integrated context. It combines geochemical modeling with numerical models that incorporate two distinct density levels of natural fractures. The interactions between the carbonate matrix, the formation brine, and the injected CO2 can lead to water vaporization and the deposition of evaporite minerals, known as the halite scale, within the porous medium. These minerals can be transported within highly conductive fractures that possess a permeability 100 times greater than the matrix. The study findings indicate that the fractures become filled, creating a natural seal that prevents CO2 leakage through what was previously considered a potential pathway in both models and different initial pH values. Furthermore, the results demonstrate the formation of secondary minerals through the reaction of CO2 and its aqueous counterparts with rock matrix minerals. These minerals, including Dawsonite, are then deposited within fracture apertures, compensating for the dissolution of calcite from the matrix and reducing the risk of enhanced fracture conductivity during CCS operations. The deposition of halite scales in initially acidic-brine saturated aquifers and/or Dawsonite scales in initially alkaline-brine saturated aquifers serves to partially counterbalance the permeability enhancement resulting from calcite dissolution. This phenomenon makes carbonate rocks a more secure option and highlights their potential suitability for CCS projects. The existence of these scales acts as a protective barrier, reducing the risk of increased permeability and enhancing the integrity of the storage reservoir.

Safety evaluation of aqueous extract from Eugenia uniflora leaves: Acute and subacute toxicity and genotoxicity in vivo assays.

ETHNOPHARMACOLOGICAL RELEVANCE: Eugenia uniflora Linn (Myrtaceae) is the native species of Brazil. The leaves of this species are used in folk medicine to treat different inflammatory and gastrointestinal disorders. However, research on the safety of using E. uniflora leaves has been poorly explored. AIM OF THE STUDY: This approach aims to investigate the phytochemical composition as well as the acute, subacute toxicity, and in vivo genotoxic profile of the aqueous extract of E. uniflora leaves. MATERIALS AND METHODS: The chemical composition of E. uniflora leaf extract was determined by Fingerprint by High-Performance Thin Layer and High-Performance Liquid Chromatography. The acute toxicity in vivo was evaluated for 14 days after the administration of E. uniflora leaves extract (2000 mg/kg). For the evaluation of subacute toxicity, mice were daily treated for 28 days with E. uniflora extract (250, 500, or 1000 mg/kg). Signs of behavioral toxicity and biochemical and hematological alterations, including the multiple organ toxicities were investigated. In addition, the micronucleus assay was used to evaluate the in vivo genotoxicity of the leaves extract in murine erythrocytes. RESULTS: The phytochemical analysis showed the majority presence of phenolic compounds (gallic acid, ellagic acid, and myricitrin). Single or repeated doses of the aqueous extract of E. uniflora leaves did not reveal any signs of in vivo toxicity. Daily doses of the extract for 28 days induced a slight reduction in cholesterol and triglyceride levels. Furthermore, E. uniflora leaves extract (1000-2000 mg/kg) showed no genetic damage in the micronucleus assay, indicating the absence of genotoxicity of the herbal species. CONCLUSION: The aqueous extract of E. uniflora leaves showed a predominance of phenolic compounds, with non-toxic and non-genotoxic action in vivo. This approach sheds light on the chemical composition of the leaves of E. uniflora and suggests a high margin of safety in the popular use of the leaves of this plant species.

Chemical and antibacterial analysis of Cinnamomum verum leaves extract and fractions against multidrug resistant bacteria.

The chemical composition, the antioxidant and antimicrobial potential of crude extract from leaves Cinnamomum verum and their enriched fractions was studied. Phytochemical analyses were performed by TLC and HPLC, and the antioxidant capacity was verified by DPPH• and ABTS•+. The Minimal Inhibitory/Bactericidal Concentration was conducted against twenty-two bacteria to select five strains susceptible to extracts/fractions and resistant to the antibiotics tested. Interference of Ethyl Acetate Fraction (EAF) in resistance to synthetic antibiotic was assayed by modulatory and checkerboard model. The chromatographic data showed phenolic compounds in crude extract, as well the flavonoid enrichment in the EAF. The combination of EAF and synthetic antibiotics (ampicillin, azithromycin, ciprofloxacin, or gentamicin) provides a synergistic effect against multidrug resistant strains). The results are useful to obtain multi-targeting in a single therapy solution, which on antioxidants molecules plant-derivatives can act synergistically in antimicrobial combinations, a valuable aid as bacterial resistance modifying compounds.

Brazilian overview of per- and polyfluoroalkyl substances listed as persistent organic pollutants in the stockholm convention.

PFAS are a group of organic chemicals, which some presents environmental persistence, dispersion and potential toxicity. Some of them have been listed in the Stockholm Convention as persistent organic pollutants (POPs) to have its production and use restricted, namely PFOS, its salts and PFOSF and PFOA, its salts and related substances. As a Party, Brazil has to comply with the Convention provisions regarding the control of POPs. In order to develop listed PFAS inventories in the country, the Convention guidance documents were used. Stakeholders were consulted and trade data was assessed. Complementary, a review on listed PFAS occurrence in Brazil was performed. From over 1000 queries sent, only 3 answers were received. International trade data showed an import of 93.7 tonnes of PFOSF from China and export of sulfluramid-base ant bait to other developing countries. Domestic trade data showed that around 28 t per year of EtFOSA is commercialized in Brazil. The EtFOSA sold internally could lead to emissions of up to 616 t of PFOS. With domestic and foreign trade data it was possible to estimate the production of EtFOSA from 2010 to 2018 to 40 t per year. Only 10 studies reported listed PFAS occurrence in Brazilian environmental matrices. All of them reported ubiquitous occurrence of listed PFAS in the country, being PFOS the predominant in terms of occurrence and concentration. Brazil needs to develop strategies to overcome the low engagement of stakeholders and enforce control over listed PFAS foreign trade.

Regulation of α-expansins genes in Arabidopsis thaliana seeds during post-osmopriming germination.

Seed osmopriming is a pre-sowing treatment that involves limitation of the seed water imbibition, so that pre-germinative metabolic activities proceed without radicular protrusion. This technique is used for improving germination rate, uniformity of seedling growth and hastening the time to start germination. In Arabidopsis thaliana, seed germination has been associated with the induction of enzymes involved in cell wall modifications, such as expansins. The α-expansins (EXPAs) are involved in cell wall relaxation and extension during seed germination. We used online tools to identify AtEXPA genes with preferential expression during seed germination and RT-qPCR to study the expression of five EXPA genes at different germination stages of non-primed and osmoprimed seeds. In silico promoter analysis of these genes showed that motifs similar to cis-acting elements related to abiotic stress, light and phytohormone responses are the most overrepresented in promoters of these AtEXPA genes, showing that their expression is likely be regulated by intrinsic developmental and environmental signals during Arabidopsis seed germination. The osmopriming conditioning had a decreased time and mean to 50% germination without affecting the percentage of final seed germination. The dried PEG-treated seeds showed noticeable high mRNA levels earlier at the beginning of water imbibition (18 h), showing that transcripts of all five EXPA isoforms were significantly produced during the osmopriming process. The strong up-regulation of these AtEXPA genes, mainly AtEXPA2, were associated with the earlier germination of the osmoprimed seeds, which qualifies them to monitor osmopriming procedures and the advancement of germination.

Evaluation of Root Perforation Treatments with Mineral Trioxide Aggregate: A Retrospective Case Series Study.

This study aimed to evaluate the long-term clinical success of the use of mineral trioxide aggregate (MTA) as a sealant material in root perforation treatments. Therefore, the dental records of 53 patients were analyzed, and treatment data was collected (age, gender, tooth location, jaw, presence or absence of radiolucent lesion, fallow up time and final radiographic/clinical assessment). All procedures were performed by a single specialist. Two examiners analyzed three radiographs from the records of each patient and classified the treatments as successful or unsuccessful. Data was analyzed statistically using parametric chi-square (P≤0.05). The examiners classified 69.8% of the cases as successful, with a follow-up time of 1-16.25 years (average: 6 years). The presence of initial radiolucent lesion was observed in 79.2% of the teeth, with a higher index of treatment in maxillary teeth (62.3%). However, the majority of successful cases were located in the maxilla (73.0%), while most unsuccessful ones were located in the mandible (62.5%) (P=0.014). There was no statistically significant difference regarding presence of previous lesions in successful (75.7%) and unsuccessful cases (87.5%) (P=0.330). In the present study, root perforations sealed with MTA had a success rate of 69.8% within 1-16.25 years. The presence of initial injury did not influence the prognosis, and maxillary teeth presented a higher success rate.

Surface Changes Of WaveOne™ and Reciproc® Instruments after Using Three Times for Preparation of Simulated Curved Canals with and without Glide Path.

Introduction: This study evaluated the occurrence of morphological changes on the surface of the instruments WaveOne™ and Reciproc® when used in the preparation of simulated curved canals with and without glide path (generated with the Pathfile™ system), after the first, second, and third uses. Materials and methods: Sixty-four resin blocks, which simulated curved root canals, were used and instrumented with a variety of instruments, grouped according to manufacturer and conditions of simulated canal preparation. Simulated canals were instrumented with WaveOne™ (GW1 group) and Reciproc® (GR1 group) according to manufacturers' recommendations, respectively. In contrast, GW2 and GR2 groups' simulated canals were submitted for construction of glide path with the PathFile™ system before the use of WaveOne™ and Reciproc® instruments, respectively. Each instrument was used three times; after each use, each instrument was analyzed by using scanning electron microscopy (cervical, middle, and apical thirds of the instrument) in order to characterize the occurrence of changes (fracture, twist, and crack). Data were described using means and standard deviations. We used generalized linear models to compare differences between factors (region, manufacturer, glide path, and number of uses). SPSS-15 software was used, with a significance level of 5%. Results: Without glide path, WaveOne™ instruments tended to fracture more frequently (P=0.003), twist more frequently (P=0.05), and crack more frequently (P=0.022), with increasing use, with statistically significant differences. With glide path, both WaveOne™ and Reciproc® instruments cracked less frequently (P=0.001); Reciproc® instruments did not exhibit superficial changes, such as fractures and/or twists. Conclusion: In this in vitro study Reciproc® instruments exhibited superior performance, compared with WaveOne™ instruments, particularly when glide path with the PathFile™ system was used; both instruments may be used, safely, three times to prepare curved canals.

Prevalence of late-life depression and its correlates in a community-dwelling low-educated population aged 75+ years: The Pietà study.

BACKGROUND: The number of individuals with advanced age is growing worldwide, especially in developing countries. Depression is the most common mental disorder in the elderly. The aim of this study was to evaluate the prevalence rates and the correlates of late-life depression (LLD) and clinically significant depressive symptoms (CSDS) in a population aged 75+ years. METHODS: We evaluated 639 community-dwelling individuals aged 75+ years in Caeté (MG), Brazil, and used the MINI to diagnose LLD according to DSM-IV criteria and the GDS-15 to identify CSDS. Quality of life was assessed by the WHOQOL-OLD scale. RESULTS: Overall, 639 individuals (64% female, with a mean age of 81.1 ±â€¯5.2 and 2.6 ±â€¯2.8 years of schooling) were evaluated; 70 (11.1%) were diagnosed with LLD and 146 (25.6%) with CSDS. Depressed subjects (both with LLD and CSDS) had poorer measures of quality of life. Logistic regression analyses showed that LLD was independently associated to a history of falls/fracture, a diagnosis of cognitive impairment-no dementia, the number of regular drugs used, lack of reading habit and, inversely, to systolic blood pressure. LIMITATIONS: The use of MINI which has not been validated in the elderly. No information was available on the number of previous depressive episodes or on the age of first episode. CONCLUSION: Both dimensional and categorical diagnoses of depression were prevalent among community-dwelling oldest-old individuals. Different clinical and personal variables were associated with depression, which negatively influenced the quality of life of the affected individuals.

Prenatal development of the agouti (Dasyprocta prymnolopha Wagler, 1831): External features and growth curves.

The gestation period in agoutis can range from 104 to 120 days. Knowledge regarding the morphological characteristics of embryos and fetuses is important as a base for studies in reproduction biotechnology, such as in vitro fertilization, embryo transfer and helps in determining congenital anomalies during the development phase. Thus, given the importance and lack of information about agouti embryology, the objective of this study was to characterize the external morphology and define the biometry of embryos and fetuses, at different days of development. Nine females were submitted to daily colpocytology to identify the estrus, confirm mating and identify day zero of the gestation. When the mating was confirmed they were weighed, underwent abdominal ultrasonography and surgery was conducted on the females at the gestational ages of 25, 30, 35, 40, 45, 50, 75 and 100 days. Sixteen embryos/fetuses were weighed and measured. Agouti embryos at 25 days after mating are "C" shaped, with primitive structures, 0.4±0.01cm crown-rump and weighed 0.06±0.01g; at 30 days after mating the crown-rump was 0.95±0.07cm and weighed 0.28±0.00g; at 35 days after mating the crown-rump was 155±0.07cm and weighed 0.38±0.01g; at 40 days after mating the crown-rump was 2.25±0.21cm and weighed 1.25±0.07g; at 45 days after mating the crown-rump was 3.45±0.35cm and weighed 2.75±0.64g; at 50 days after mating the crown-rump was 5.0±0.3cm and weighed 7.01±2.6g; at 75 days after mating, the skin was dark, the crown-rump was 10.0±0.14cm and weighed 55.2±0.07g. At 100 days after mating, the crown-rump was 13.8±0.49cm and fetuses weighed 136.7±9.40g. Based on the morphological data assessed the embryo and fetus age could be assessed and the size and average weight of agouti embryos was established.

Splenic rupture in dengue hemorrhagic fever: report of a case and review.

Dengue is currently the most important arboviral disease in the world, particularly in tropical countries in which the environmental conditions favor the development and proliferation of the mosquito vector. Dengue hemorrhagic fever presents in two phases: an initial phase, which is characterized by sudden onset of fever and a variety of nonspecific signs and symptoms, and a critical phase, which is characterized by the recovery from fever and development of hemorrhagic symptoms and circulatory insufficiency. This report documents a case of splenic rupture in a patient with dengue hemorrhagic fever who developed hypovolemic shock and subsequently died. Although splenic rupture is a known complication of other acute infections, it is a rare complication of dengue; therefore, it may be misdiagnosed. In the case described here, the poor outcome mainly resulted from the sudden onset of complications; the patient died of splenic rupture less than 24 h after admission, and the cause of death was confirmed at necropsy.

[Mortality of malnourished elderly in Belo Horizonte, Minas Gerais State, Brazil: a multidimensional analysis focusing on multiple causes of death]. / Mortalidade de idosos com desnutrição em Belo Horizonte, Minas Gerais, Brasil: uma análise multidimensional sob o enfoque de causas múltiplas de morte.

This study aimed to identify the mortality profiles of elderly individuals with malnutrition, based on associations between multiple causes of death and other variables recorded on the death certificate. We studied the deaths of elderly (> 60 years) living in Belo Horizonte, Minas Gerais State, Brazil, with malnutrition recorded as one of the causes. Data were used from the mortality information system. Multiple correspondence analysis was used to investigate these associations. The study showed excess mortality among women, whites, widowers, and individuals with low schooling. Ten mortality profiles were studied, and malnutrition was five times more frequent according to the multiple-causes technique. The results showed both the importance of multiple causes of death not detected by focusing on the underlying cause and the adequacy of multiple correspondence analysis for identifying the associations between causes and other variables listed on the death certificate.

Mortalidade de idosos com desnutrição em Belo Horizonte, Minas Gerais, Brasil: uma análise multidimensional sob o enfoque de causas múltiplas de morte / Mortality of malnourished elderly in Belo Horizonte, Minas Gerais State, Brazil: a multidimensional analysis focusing on multiple causes of death

O objetivo deste estudo foi identificar perfis de mortalidade em idosos com desnutrição, a partir de associações entre causas múltiplas de morte e outras variáveis da Declaração de Óbito. Foram estudadas as mortes de residentes de Belo Horizonte, Minas Gerais, Brasil, com 60 anos e mais, que tinham a desnutrição como uma das causas mencionadas. Utilizaram-se os dados do Sistema de Informações sobre Mortalidade. Para estudo das associações, foi empregada a análise multidimensional pela técnica de análise fatorial de correspondência múltipla. A pesquisa mostrou sobremortalidade feminina, maior concentração de mortes na raça branca, em viúvos e em idosos com baixa escolaridade. Foram estabelecidos dez perfis de mortalidade e a desnutrição foi evidenciada cerca de cinco vezes mais pelo enfoque das causas múltiplas. Confirmou-se a importância das causas múltiplas para evidenciar causas de morte não detectadas pelo enfoque de causa básica e a adequação da técnica de análise de correspondência múltipla para identificar as associações de causas e demais variáveis da Declaração de Óbito.

This study aimed to identify the mortality profiles of elderly individuals with malnutrition, based on associations between multiple causes of death and other variables recorded on the death certificate. We studied the deaths of elderly (> 60 years) living in Belo Horizonte, Minas Gerais State, Brazil, with malnutrition recorded as one of the causes. Data were used from the mortality information system. Multiple correspondence analysis was used to investigate these associations. The study showed excess mortality among women, whites, widowers, and individuals with low schooling. Ten mortality profiles were studied, and malnutrition was five times more frequent according to the multiple-causes technique. The results showed both the importance of multiple causes of death not detected by focusing on the underlying cause and the adequacy of multiple correspondence analysis for identifying the associations between causes and other variables listed on the death certificate.

Type 1 diabetes susceptibility determined by HLA alleles and CTLA-4 and insulin genes polymorphisms in Brazilians.

INTRODUCTION: Type 1A diabetes mellitus (T1ADM) is a multifactorial disease in which genetic and environmental aspects are important to its development. The association of genetic variations with disease has been demonstrated in several studies; however, the role of some gene loci has not yet been fully elucidated. OBJECTIVE: To compare the frequency of HLA alleles and polymorphism in CTLA-4 and insulin genes in Brazilians with T1ADM and individuals without the disease, as well as to identify genetic markers that are able to discriminate between diabetic and non-diabetic individuals. METHODS: The presence of HLA DQB1, DQA1 and DRB1 alleles, as well as the -2221 MspI polymorphism in the insulin gene and 49 A/G in the CTLA-4 gene were identified by the 'Time-resolved fluorometer' technique after hybridization with probes labeled with Eu (III) / Sm (III) and Tb (III). RESULTS: The DQB1 *0302 and DQA1 *03 alleles were identified as predisposed to T1ADM, and the DQB1 *0301 allele presented a protective effect against the disease.The DQA1 label proved to be able to differentiate between 71.13% of the diabetic and non-diabetic individuals.This value increased to 82.47% when the DQB1 label was added. No significant difference in the frequency of polymorphisms in the insulin and CTLA-4 genes was observed between the two groups. CONCLUSIONS: The genetic markers that best characterized and discriminated diabetic and non-diabetic individuals were the HLA DQA1 and DQB1.alleles.

Type 1 diabetes susceptibility determined by HLA alleles and CTLA-4 and insulin genes polymorphisms in Brazilians / Suscetibilidade ao diabetes tipo 1 determinada por alelos de HLA e polimorfismos nos genes CTLA-4 e insulina em brasileiros

INTRODUCTION:Type 1A diabetes mellitus (T1ADM) is a multifactorial disease in which genetic and environmental aspects are important to its development. The association of genetic variations with disease has been demonstrated in several studies; however, the role of some gene loci has not yet been fully elucidated. OBJECTIVE:To compare the frequency of HLA alleles and polymorphism in CTLA-4 and insulin genes in Brazilians with T1ADM and individuals without the disease, as well as to identify genetic markers that are able to discriminate between diabetic and non-diabetic individuals. METHODS: The presence of HLA DQB1, DQA1 and DRB1 alleles, as well as the -2221 MspI polymorphism in the insulin gene and 49 A/G in the CTLA-4 gene were identified by the "Time-resolved fluorometer" technique after hybridization with probes labeled with Eu (III) / Sm (III) and Tb (III). RESULTS: The DQB1 *0302 and DQA1 *03 alleles were identified as predisposed to T1ADM, and the DQB1 *0301 allele presented a protective effect against the disease.The DQA1 label proved to be able to differentiate between 71.13 percent of the diabetic and non-diabetic individuals.This value increased to 82.47 percent when the DQB1 label was added. No significant difference in the frequency of polymorphisms in the insulin and CTLA-4 genes was observed between the two groups. CONCLUSIONS: The genetic markers that best characterized and discriminated diabetic and non-diabetic individuals were the HLA DQA1 and DQB1.alleles.

INTRODUÇÃO: O diabetes melito tipo 1 (T1ADM) é uma doença multifatorial em que os aspectos genéticos e ambientais são importantes para o seu desenvolvimento. A associação das variações genéticas com a doença tem sido demonstrada em vários trabalhos, no entanto, o papel de alguns locos gênicos não foi ainda completamente elucidado. OBJETIVOS: Comparar a frequência de alelos do HLA e polimorfismos nos genes CTLA-4 e insulina em brasileiros com T1ADM e indivíduos sem a doença, além de identificar marcadores gênicos que sejam capazes de discriminar indivíduos diabéticos e não diabéticos. MÉTODOS: A presença dos alelos de HLA DQB1, DQA1 e DRB1, bem como dos polimorfismos -2221 MspI no gene da insulina e 49 A/G no gene CTLA-4, foram identificados por meio da técnica Time-resolved fluorometer, após hibridização com sondas marcadas com Eu (III)/Sm (III) e Tb (III). RESULTADOS: Os alelos DQB1*0302 e DQA1*03 foram identificados como sendo de predisposição ao T1ADM, e o alelo DQB1*0301 mostrou um efeito protetor à doença. Analisando somente o marcador DQA1, este mostrou ser capaz de diferenciar 71,13 por cento dos indivíduos entre diabéticos e não diabéticos, cujo valor aumentou para 82,47 por cento quando adicionado o marcador DQB1. A frequência dos polimorfismos nos genes da insulina e CTLA-4 não mostrou diferença significativa entre os dois grupos estudados. CONCLUSÕES: Os marcadores genéticos que melhor caracterizaram e discriminaram diabéticos e não diabéticos foram os alelos de HLA DQA1 e DQB1.

Determining IgA and IgG antigliadin, IgA antitransglutaminase, and antiendomysial antibodies in monkey esophagus and in umbilical cord for diagnosis of celiac disease in developing countries.

OBJECTIVES: To assess the efficiency of determining IgA and IgG antigliadin antibodies (IgA- and IgG-AGA, respectively), antitransglutaminase (TgA), and anti-endomysial antibodies (AEA) in human umbilical cord (CO) and monkey esophagus for diagnosis of celiac disease; to determine the correlation between serological markers and celiac disease. PATIENTS AND METHODS: A total of 400 patients were divided in 3 groups: group 1 with 37 patients with celiac disease, group 2 with 208 patients with no enteropathies, and group 3 with 155 patients with other enteropathies. IgA-AGA, IgG-AGA, and TgA were assessed using enzyme-linked immunosorbent assay, whereas AEA was evaluated by indirect immunofluorescence. RESULTS: Sensitivity and specificity of IgA-AGA were 81.1% and 95.2%, of IgG-AGA 89.2% and 95.2%, of TgA 83.9% and 96.8%, of AEA-CO 87.9% and 100%, and of AEA of monkey esophagus 88.6% and 100%, respectively. Positive predictive values were 75.0%, 76.7%, 83.9%, and 100%. Negative predictive values were 96.6%, 98.0%, 96.8%, and 97.7% for IgA-AGA, IgG-AGA, TgA, and AEA, respectively. Multivariate analysis showed a strong association between AEA-CO and celiac disease and a good correlation with other markers (TgA, IgA-AGA, and IgG-AGA). CONCLUSIONS: TgA has been recommended for screening patients with celiac disease. Considering the similar sensitivity and specificity of IgA-AGA and TgA and their correlations in the multivariate analysis, both are applicable for this purpose. However, because TgA tests are highly costly and celiac disease is associated with IgA deficiency, the determination of IgA-AGA and IgG-AGA, followed by AEA-CO, is suitable for screening in developing countries, provided a cutoff point for these examinations is established. The results of antiendomysial antibodies in umbilical cord overlapped those in monkey esophagus. Therefore, umbilical cord should be used as a substrate instead of specimens from endangered species.