Simultaneous determination of four antipsychotic drugs in plasma by high-performance liquid chromatography. Application to management of acute intoxications.

A specific reversed phase-high pressure liquid chromatography (RP-HPLC) method has been developed for the simultaneous determination of clozapine (CZP), loxapine (LXP), zuclopenthixol (ZPT) and flupenthixol (FPT) in plasma. These four antipsychotic drugs are frequently used for the treatment of schizophrenia and other neuropsychiatric diseases. Carpipramine, a dihydrodibenzazepine, was used as an internal standard (I.S.). A liquid-liquid procedure was used to extract the drugs from human plasma. The analysis was performed on a XTerra MS C18 column with UV detection. Calibration curves were linear in the range 50-1000 microg/l. The limit of quantification (LOQ) was 15 microg/l for clozapine and loxapine and 20 microg/l for zuclopenthixol and flupenthixol. The coefficient of variation (CV) for intra- and inter-day precision was 7.2% or less with accuracies within 10% for the three concentrations.This isocratic and rapid method (run time<10 min) is useful for the management of acute intoxication.

[Genealogical approach to the formation of the winter wheat core collection]. / Genealogicheskii podkhod k formirovaniiu sterzhnevoi kollektsii ozimoi pshenitsy.

The correctness of the genealogical approach to the estimation of genetic diversity has been substantiated. The approach implies a comparison of the estimates of similarity based on genetic markers and coefficients of parentage. A method of the application of the genealogical approach to the formation of the core collection has been developed. The adequacy of the core set has been estimated using Shannon's diversity index adapted for genetic profiles and the correlation between the distributions of ancestors' contributions. A core collection has been formed. This collection contains 25% of the accessions of the original winter wheat (Triticum aestivum L.) collection from the Czech Genebank and represents 70-76% of the intracluster diversity. A decrease in intercluster diversity has not been found in the core collection.

Rapid HPLC measurement of buflomedil in plasma in poisoning cases.

A rapid high-performance liquid chromatographic method for the determination of buflomedil in human plasma is described. It requires a single liquid-liquid extraction step from 1 mL of plasma with diethyl ether followed by chromatography on a Nova Pak C(18) reversed-phase column and detection by ultaviolet light. Metoclopramide was used as internal standard. The method is sensitive with a quantification limit at 500 ng/mL. It was used for the determination of buflomedil in biological fluids in poisoning cases.