RESUMO
Lymphokine-activated (LK+) and control (LK-) macrophages were cultured for 66 h and then pulsed with [14C]glucosamine. Uptake of [14C]glucosamine was greater in LK+ than in LK- cultures. If, after 66 h, the medium was replaced with fresh medium and then pulsed with either [14C]glucose or [14C]glucosamine, the uptake of isotope was greatly reduced compared to cultures with no change of medium. However, uptake of both radiolabeled substances was still found to be greater in LK+ cultures than in LK- cultures. Although uptake of both substances was enhanced by lymphokines, the uptake kinetics of each isotope was different. Under similar conditions the uptake of [3H]leucine was not enhanced by lymphokine activation. These data are interpreted to mean that LK+ macrophages are metabolically stimulated and utilize more glucose and glucosamine. The difference in kinetics implies a different utilization by macrophages for each substance.
Assuntos
Glucosamina/metabolismo , Glucose/metabolismo , Leucina/metabolismo , Linfocinas/metabolismo , Macrófagos/metabolismo , Animais , Líquido Ascítico/citologia , Radioisótopos de Carbono , Cobaias , Masculino , TrítioRESUMO
A new latex-inhibition slide test for pregnancy, Gest female State, with the use of polystyrene latex particles to which human chorionic gonadotropin (hCG) has been bound, is described. The sensitivity of this test was found to be 2 to 4 1.U. of hCG per milliliter of urine. Clinical trials with the Gest female State in urine samples from 328 patients (203 positive, 125 negative) showed one false negative and no false positive results. Urine containing drugs or high concentrations of various ingredients and abnormal urine did not result in interference with expected test results. The new test was comparable with or superior to other commercially available rapid slide tests for pregnancy.