Determination using impedance cardiograph of the chronic effects of different doses of radiotherapy on the cardiovascular system of rats.

AIM: Cardiac damage caused by radiation in the long term varies according to the radiation dose received by the heart. In this study, it was aimed to evaluate the damage caused by different radiation doses in the heart, together with hemodynamic parameters, immunhistochemistry, and histopathological analyzes for long term. METHOD AND MATERIALS: The animals were divided into four groups: The rats in control group (Group 1) were not irradiated; the rats in group 2 were irradiated with 5 Gy; the rats in group 3 were irradiated with 10 Gy and the rats in group 4 were irradiated with 20 Gy. Hemodynamic parameters and indices were determined from the impedance cardiography (ICG) recording in the whole groups before they were irradiated with RT and 180 days after RT. And then, interleukin-1ß, interleukin-10, TNF-α, apopthosis were determined in all groups. In addition, histological changes of heart and aorta were evaluated. RESULTS: Histopathologic, cytokine and hemodynamic findings supported that cardiac damage increased with increasing radiation dose. CONCLUSION: it is important in terms of being an alternative and supportive method to other methods to be able to detect heart diseases caused by RT with the ICG method.

Impact of different fixed dental prostheses on radiation dose in helical tomotherapy as measured with metal oxide semiconductor field-effect transistor dosimetry.

OBJECTIVE: This ex vivo study evaluated the effects of different fixed dental prostheses and protective materials on scattered radiation during radiation therapy (RT). STUDY DESIGN: Natural teeth (group NT) and 4 types of prostheses (group BL: bilayer lithium disilicate glass-ceramic; group MZ: monolithic zirconia ceramic; group BZ: bilayer zirconia-based all ceramic; and group BM: bilayer metal-ceramic restorations) were examined in maxillary and mandibular arch phantoms. All groups were divided into 3 subgroups: (1) without protective material over the prostheses; (2) protected with a soft acrylic night guard; and (3) protected with polyvinylsiloxane putty. All groups were irradiated with helical RT at 2 Gy and 6 MV photon energy. Doses were measured internally and externally 3 times on each phantom. Results were significant at P < .05. RESULTS: No statistically significant differences in doses were found between groups NT and BL in either phantom, but these groups were different from the others. The differences between groups BZ and MZ were insignificant. Doses in group BM were significantly larger than all other groups. No significant differences existed between doses with and without protective material. CONCLUSIONS: Doses increased significantly due to scattered radiation from the prostheses with increasing material density. Protective materials had no significant effect on dose.

Protective effect of N-acetyl cysteine against radiotherapy-induced cardiac damage.

Purpose: Although radiotherapy (RT) is an important component of cancer treatment, it induces adverse tissue reactions in the around of cancer tissue. Therefore, radioprotectives are needed to protect normal tissues. The aim of this study was to investigate the radioprotective effect of N-acetylcysteine (NAC) on RT-induced cardiac damage in rats for the acute term.Materials and methods: The animals were divided into four groups. The rats in control group were injected with saline for 7 d; the rats in NAC group were injected NAC at dose of 240 mg/kg d for 7 d; the rats in RT group were injected with saline for 7 d plus was irradiated 1 h after the last injection and the rats in NAC + RT group were injected with NAC for 7 d and irradiated 1 h after the last NAC dose. The electrocardiogram was recorded and evaluated PR interval, QRS duration, QT interval, T wave alterations and heart rate. Serum interleukin-4, interleukin-6, tumor necrosis factor-alpha, interleukin 1 beta, galectin-3 levels and creatine kinase and creatine kinase isoenzyme-MB activities were determined in all groups. Also, tissue malondialdehyde (MDA) and nitric oxide levels, superoxide dismutase, catalase and glutathione peroxidase activities were determined. In addition, histological changes of heart were evaluated. All measurements were performed 24 h after RT.Results: In the RT group, findings supporting cardiac injury were observed in the electrocardiogram. Also, cytokine levels and oxidative stress were significantly increased. Pretreatment of rats with NAC ameliorated cardiac injury induced by RT.Conclusions: Our findings suggested that NAC may be a potential radioprotector which is capable of preventing cardiac damage.

Effect of Imipramine on radiosensitivity of Prostate Cancer: An In Vitro Study.

Prostate cancer is the most common cancer and leading cause of cancer death for males. Imipramine (IMI), which is a tricyclic antidepressant, has also been shown to has antineoplastic effect. This study was performed to investigate the radiosensitizing effect of IMI on DU145 prostate cancer cell. Cells were divided into 4 groups. Cell index, apoptotic activity, cell cycle arrest, oxidative stress and EAG1 channel currents were determined in all groups. Our findings showed that combined treatment with IMI and radiotherapy (RAD) did not enhance radiosensitivity of DU145 cells but as unexpected finding, treatment of IMI alone was more effective in DU145 cells.