Bacterial Spermosphere Inoculants Alter N. benthamiana-Plant Physiology and Host Bacterial Microbiome.

In this study, we investigated the interplay between the spermosphere inoculum, host plant physiology, and endophytic compartment (EC) microbial community. Using 16S ribosomal RNA gene sequencing of root, stem, and leaf endophytic compartment communities, we established a baseline microbiome for Nicotiana sp. Phenotypic differences were observed due to the addition of some bacterial inoculants, correlated with endogenous auxin loads using transgenic plants expressing the auxin reporter pB-GFP::P87. When applied as spermosphere inoculants, select bacteria were found to create reproducible variation within the root EC microbiome and, more systematically, the host plant physiology. Our findings support the assertion that the spermosphere of plants is a zone that can influence the EC microbiome when applied in a greenhouse setting.

Conocimientos en lactancia materna y alimentación complementaria en madres usuarias de los servicios de salud pública de Guatemala / Knowledge of breastfeeding and complementary feeding in mothers who use public health services in Guatemala

: Las prácticas adecuadas de lactancia materna (LM) y de alimentación complementaria (AC) contribuyen al crecimiento y desarrollo de los niños. Con el objetivo de determinar el nivel de conocimientos en LM y AC en madres usuarias de los Servicios de Salud Pública (SSP) de Guatemala, se realizó un estudio descriptivo y transversal, esperando que los resultados sean utilizados para diseñar intervenciones basadas en evidencia. Se diseñó un instrumento de recolección de datos, el cual se utilizó en una muestra de 1,956 madres entre 15 a 49 años. Se entrevistó a madres de niños menores de dos años usuarias de los SSP de los lugares de cobertura del Ejercicio Profesional Supervisado de la Universidad de San Carlos de Guatemala. Los datos se analizaron mediante estadística descriptiva, se calificó cada pregunta en cero (0) para respuesta incorrecta y uno (1) para respuesta correcta, estableciendo el nivel de conocimientos como alto (67 a 100% respuestas correctas), medio (34 a 66% respuestas correctas) o bajo (0 a 33% respuestas correctas), agrupando los resultados según variables sociodemográficas. El nivel de conocimiento sobre LM fue alto en 989 madres (51%), medio en 888 madres (45%) y bajo en 79 madres (4%) con un promedio de nota de 73.2(17.1)[58.2, 88.2] sobre 100 puntos. En cuanto al nivel de conocimiento sobre AC fue alto en 251 madres (13%), medio en 1,608 madres (82%) y bajo en 97 madres (5%) con un promedio de nota de 55.2(12.1)[44.5, 65.8]. Se concluyó que, a menor edad y escolaridad de la madre, mayor porcentaje de nivel bajo de conocimiento en LM y AC.

Appropriate breastfeeding (LM ,from its Spanish initials) and complementary feeding (AC, from its Spanish initials) practices contribute to the growth and development of children. The objective of this work was to determine the level of knowledge in LM and AC in mothers who use Public Health Services (SSP) in Guatemala. A descriptive and cross-sectional study was carried out, hoping that the results would be used to design an evidence-based impact. A data collection instrument was built, which was used in a sample of 1,956 mothers between 15 and 49 years of age. Mothers of children under two years of age who are users of the SSP covered by the Nutrition Supervised Professional Practice of the University of San Carlos of Guatemala were interviewed. The data was analyzed using descriptive statistics, each question was scored zero (0) for an incorrect answer and one (1) for a correct answer, establishing the level of knowledge as high (67 to 100% correct answers), medium (34 to 66% correct answers) or low (0 to 33% correct answers), grouping the results obtained according to sociodemographic variables. The level of knowledge about LM was high in 989 mothers (51%), medium in 888 mothers (45%), and low in 79 mothers (4%) with an average grade of 73.2(17.1)[58.2, 88.2] of a 100 points. Regarding the level of knowledge about AC, it was high in 251 mothers (13%), medium in 1,608 mothers (82%) and low in 97 mothers (5%) with an average grade of 55.2(12.1)[44.5, 65.8] . It was concluded that the lower the age and schooling of the mother, the higher the percentage of low level of knowledge in LM and AC.

Parental stress and life satisfaction: A comparative study of social services users and nonusers from a gender perspective.

The psychometric properties of the Spanish version of the Parental Stress Scale (PSS) scale have not been verified on the Spanish population. Similarly, the literature on gender differences and parental stress is inconclusive, and there is little evidence of their relationship with life satisfaction. To analyze the psychometric properties of the Spanish version of the PSS scale, (2) to examine possible gender differences, and (3) to study the relationship between parental stress (PS) and parental rewards (PR) and satisfaction with life (SWL) attending to the possible moderating effect of gender. These objectives were examined in samples comprising Social Services Users (SSU) (N = 525; 78.3% female; Mage = 38.3) and non-SSU users (N = 421; 41.1% male; Mage = 37.08). A CFA corroborated a two-factor structure: PS and PR. In the SSU sample, mothers showed higher PS and lower PR. However, PR was also higher in mothers from the non-SSU sample compared to fathers. PR and PS were directly related to SWL in the SSU sample. However, gender moderated the relationship between PR and SWL in the non-SSU sample in the case of mothers. The results are discussed considering gender roles and the characteristics of both samples.

Rol del resentimiento en la reconciliación social: víctimas movilizadas en Colombia (2021) / Role of resentment in social reconciliation: Mobilisation of victims in Colombia (2021)

Resumen Introducción: diez años después de la implementación de la Ley 1448 de 2011 para la reparación integral de las víctimas en Colombia desarrollamos una investigación cuantitativa no experimental para explorar la interacción entre sentimientos hacia la reconciliación (coexistencia y reconstrucción de la confianza) y resentimiento duradero en víctimas del conflicto movilizadas colectivamente en escenarios de justicia transicional. Método: utilizamos datos de dos muestras de participantes de los departamentos de Caldas (N = 342) y Atlántico (N = 305). Resultados: confirmamos las escalas sobre sentimientos hacia la reconciliación y resentimiento duradero, cuya fiabilidad fue analizada mediante los coeficientes alfa de Cronbach y omega. Posteriormente, realizamos un análisis de ecuación estructural y confirmamos las siguientes hipótesis: (1) las víctimas experimentan más sentimientos de coexistencia que sentimientos para la reconstrucción de confianza, y (2) el resentimiento duradero es un obstáculo para desarrollar sentimientos de reconciliación. Conclusiones: los participantes experimentan más sentimientos de coexistencia y confianza que resentimiento duradero; y la coexistencia permite canalizar el resentimiento duradero. Estos hallazgos tienen implicaciones considerables en el desarrollo de sentimientos hacia la reconciliación en escenarios de justicia transicional.

Abstract Introduction: Ten years after the implementation of Law 1448 of 2011 for the comprehensive reparation of victims in Colombia, we developed non-experimental quantitative research to explore the interaction between feelings towards reconciliation (coexistence and reconstruction of trust) and the lasting resentment in victims of the conflict mobilized collectively in transitional justice scenarios. Method: We used data from two samples of participants from the departments of Caldas (N = 342) and Atlántico (N = 305). Results: We confirmed the scales on feelings toward reconciliation and lasting resentment, whose reliability was analyzed using Cronbach's alpha and omega coefficients. Subsequently, we performed a structural equation analysis and confirmed the following hypotheses: (1) victims experience more feelings of co-existence than feelings for rebuilding trust, and (2) Lasting resentment is an obstacle to developing feelings towards reconciliation. Conclusions: We highlight that participants experience more feelings of coexistence and trust than they do lasting resentment; and that coexistence allows for the channeling of lasting resentment. These findings have considerable implications for the development of feelings towards reconciliation in transitional justice settings.

Introduction of loxP sites by electroporation in the mouse genome; a simple approach for conditional allele generation in complex targeting loci.

BACKGROUND: The discovery of the CRISPR-Cas9 system and its applicability in mammalian embryos has revolutionized the way we generate genetically engineered animal models. To date, models harbouring conditional alleles (i.e. two loxP sites flanking an exon or a critical DNA sequence of interest) are amongst the most widely requested project type that are challenging to generate as they require simultaneous cleavage of the genome using two guides in order to properly integrate the repair template. An approach, using embryo sequential electroporation has been reported in the literature to successfully introduce loxP sites on the same allele. Here, we describe a modification of this sequential electroporation procedure that demonstrated the production of conditional allele mouse models for eight different genes via one of two possible strategies: either by consecutive sequential electroporation (strategy A) or non-consecutive sequential electroporation (strategy B). This latest strategy originated from using the by-product produced when using consecutive sequential electroporation (i.e. mice with a single targeted loxP site) to complete the project. RESULTS: By using strategy A, we demonstrated successful generation of conditional allele models for three different genes (Icam1, Lox, and Sar1b), with targeting efficiencies varying between 5 and 13%. By using strategy B, we generated five conditional allele models (Loxl1, Pard6a, Pard6g, Clcf1, and Mapkapk5), with targeting efficiencies varying between 3 and 25%. CONCLUSION: Our modified electroporation-based approach, involving one of the two alternative strategies, allowed the production of conditional allele models for eight different genes via two different possible paths. This reproducible method will serve as another reliable approach in addition to other well-established methodologies in the literature for conditional allele mouse model generation.

Work-Family Guilt in Spanish Parents: Analysis of the Measurement, Antecedents and Outcomes from a Gender Perspective.

This research work had three objectives: (1) to analyze the psychometric properties of the Spanish version of the Work-Family Guilt Scale, (2) to examine its invariance according to gender, and (3) to study the relationship between work-family guilt (WFG) and the different proposed antecedent (e.g., hours spent working, social support, rumination, and personality) or consequential factors (e.g., life satisfaction), noting any gender differences. The incidental sample comprised 225 parents who were in paid work and had at least one child attending nursery school (49.1% women; age of total sample = 36.88 on average). Multiple-group and confirmatory factor analyses, correlations, multiple regression, and moderation analyses were carried out. The WFGS reflected the same factorial structure in men and women, with two main factors: work interfering with family guilt (WIFG) and family interfering with work guilt (FIWG). No gender differences were found. The discrepancy associated with perfectionism was the only variable that was found to be a predictor of FIWG. The major predictors of WIFG were brooding from rumination and the number of hours spent working. WIFG was also associated with lower life satisfaction in women. The implications of these results are discussed, stressing the need to promote work-family reconciliation policies.

Toxicity evaluation of hospital laundry wastewaters treated by microbial fuel cells and constructed wetlands.

Hospital laundries generate high wastewater volumes with the presence of several contaminants. Nevertheless, few studies have investigated the toxicity of these effluents and looked for treatment alternatives that might reduce this eventual toxicity. So, the present study assessed the performance of an integrated system combining a microbial fuel cell (MBFC) and a constructed wetland (CW) to reduce toxic effects of wastewaters generated at a hospital laundry. After collection, raw effluents remained 7 days at the first unit (MBFC) of integrated system. Afterward, they were transferred to the second unit (CW) unit where remained more 7 days totaling a hydraulic detention time of 14 days. The toxicity evaluation involved three different organisms: Daphnia magna (acute ecotoxicity), Lactuca sativa (phytotoxicity) and Allium cepa (phytotoxicity, cytotoxicity, mutagenicity, and genotoxicity). Got results revealed an extremely acute ecotoxicity against D. magna, high phytotoxic effects in the L. sativa and A. cepa assays, and genotoxicity in the A. cepa assay for the untreated effluents. Furthermore, no significant incidence of micronuclei was observed in the raw wastewaters. Regarding the treatment, after the first stage, it was possible to verify that MBFC reduced the toxicity of the wastewaters only in some tested assays (endpoints) while after the CW (second stage) the effluents presented a complete absence of toxicity of the investigated bioassays. Therefore, the use of the integrated system combining two environmentally friendly technologies can be considered promising, since both MBFC and CW presented a complimentary effect with excellent results regarding the reduction of the overall toxicity of hospital laundry wastewaters.

Síndrome de reconstitución inmune en pacientes VIH positivo / Immune reconstitution syndrome in HIV-positive patients

El síndrome de reconstitución inmune (SR) en pacientes VIH positivo es una consecuencia adversa de la restauración de la respuesta inmune específica, durante los primeros 6 meses de tratamiento antirretroviral de gran actividad (TARGA). Objetivo. Cuantificar la prevalencia de SRI y caracterizar demográfica, clínica, inmunológica y virológicamente a pacientes VIH positivo con SRI que iniciaron TARGA en la Clínica de Enfermedades Infecciosas del Hospital Roosevelt, ciudad capital, Guatemala, 2006-2008...