Potentially toxic elements in different tissues of great cormorant (Phalacrocorax carbo) at a wetland area.

Concentrations of 4 potentially toxic elements (As, Cd, Hg, Pb) were investigated in the feather, liver, kidney, and bone of great cormorants (Phalacrocorax carbo). The tissue samples were taken at the Central Tisza - Jászság Nature Conservation Area in Hungary. They were analysed by inductively coupled plasma optical emission spectroscopy (ICP-OES). The goal of the investigation was to analyse the metal burden of the above-mentioned elements in the various tissues of these wild birds and to provide important information for monitoring the environmental pollution.Amongst the examined potentially toxic elements no statistical gender difference was observed, so the data were not separated based on them during the statistical analysis. The concentration of mercury was the highest in the feather, followed by the liver, kidney, and bone. The lead was detected in the feather with the highest level followed by the kidney, liver, and bone. The cadmium was determined in all investigated tissues with the next descending order: kidney > bone > liver > feather. Highest arsenic concentration was measured in the feather, followed by liver, kidney, and bone with the same concentration.The detected concentrations of the investigated potentially toxic elements in different tissues of great cormorants (feathers, liver, kidney, bone) means that the living area of this birds is not highly contaminated to induce health problems or toxic signs, or even other undesirable effect in the animals.

To Eat or Not to Eat?-Food Safety Aspects of Essential Metals in Seafood.

The popularity of seafoods is high due to their superb dietary properties and healthy composition. However, it is crucial to understand whether they adequately contribute to our essential nutritional needs. Small amounts of essential metals are indispensable in the human body to proper physiological functioning; their deficiency can manifest in various sets of symptoms that can only be eliminated with their intake during treatment or nutrition. However, the excessive consumption of metals can induce undesirable effects, or even toxicosis. Shellfish, oyster, and squid samples were collected directly from a fish market. After sample preparation, the concentration of essential metals (cobalt, chromium, copper, manganese, molybdenum, nickel, and zinc) was detected by Inductively Coupled Plasma Optical Emission Spectrometry. The results were analyzed statistically using ANOVA and two-sample t-tests. The average concentration of the investigated essential elements and the calculated burden based on the consumption were below the Recommended Dietary Allowances and Tolerable Upper Intake Levels. Based on these results, the trace element contents of the investigated seafoods do not cover the necessary recommended daily intake of them, but their consumption poses no health hazard due to their low levels.

Metal Load of Potentially Toxic Elements in Tuna (Thunnus albacares)-Food Safety Aspects.

The consumption of marine fishes has a positive effect on a consumer's health; however, it poses a potential risk due to their level of heavy metals in their body. Heavy metals can be naturally found in the environment, but their concentration can be increased with anthropogenic activities. Samples of tuna (Thunnus albacares) were collected at a fishery market. The potentially toxic elements (arsenic, cadmium, lead, and mercury) were determined with a validated method in the flesh of fish using inductively plasma optical emission spectrometry after microwave digestion. Generally, the average concentration of them was below the official limit values regulated by the European Union, except for lead. Based on the concentrations of arsenic (inorganic derivates: 0.05 ± 0.02 mg/kg) and cadmium (0.03 ± 0.01 mg/kg) in the tuna fish samples, and their calculated EDI values (As: 0.03-0.09 µg/kg/day; Cd: 0.05-0.07 µg/kg/day), the investigated food could be declared safe for human consumption. Generally, mercury content was below the official regulated limit, and the calculated EDI value was below the dietary reference value (0.3 µg/kg/day) in most of the samples (90%), exceeding it only in two samples (0.69 and 0.82 µg/kg/day); thus, they may not be harmful to the consumer. The concentration of lead above the official maximum limit (0.30 mg/kg) in 40% of tuna samples (0.30-1.59 mg/kg), as well as the exceeding of the dietary reference value for lead (adult: 0.16 µg/kg/day; children: 0.26 µg/kg/day) based on the calculated EDI values (0.28-1.49 µg/kg/day), draw attention to the importance of environmental pollution and the protection of consumers' health.

Monitoring of metal content in the tissues of wild boar (Sus scrofa) and its food safety aspect.

The study was performed on 10 female and 10 male wild boars (Sus scrofa) after shooting during the regular hunting season to investigate the concentration of metals in the muscle and fat tissue. The concentrations of essential and non-essential elements were determined (arsenic (As), cadmium (Cd), chromium (Cr), copper (Cu), lead (Pb), manganese (Mn), mercury (Hg), and zinc (Zn)) using inductively coupled plasma optical emission spectrometry. The concentrations of As, Hg, and Cd were below the limit of detection (As, Hg: < 0.5 mg/kg, Cd: < 0.05 mg/kg) in every tissue sample of both sexes. The lead was detected as 0.36 ± 0.16 mg/kg and 0.22 ± 0.06 mg/kg in the muscle of females and males, respectively, showing a significant difference between the sexes (p = 0.0184). The measured concentration of Cr was 0.14 ± 0.08 mg/kg and 0.13 ± 0.06 mg/kg, and that of copper was 1.22 ± 0.14 mg/kg and 1.06 ± 0.16 mg/kg in the muscle of females and males, respectively. The same tendency was observed in the case of copper content of fat tissues (female: 0.13 ± 0.10 mg/kg; male: 0.13 ± 0.04 mg/kg; p = 0.2707). Manganese concentration of muscle was 0.45 ± 0.30 mg/kg (female) and 1.36 ± 0.96 mg/kg (male), and that of fat tissue was 0.32 ± 0.22 mg/kg (female) and 0.74 ± 0.75 mg/kg (male). The Zn was detected as 56.75 ± 7.86 mg/kg and 1.83 ± 0.76 mg/kg in the muscle and fat of females and 52.12 ± 11.51 mg/kg and 1.94 ± 0.57 mg/kg in males, respectively. Based on data, the consumption of fat and muscle tissues of the wild boars tested can be food toxicologically objectionable, mainly due to the lead content, and thus pose a risk to frequent consumers of this type of game meat.

Toxic elements in Sardina pilchardus and food toxicological significance.

Cd, Hg, and Pb concentrations were investigated in Sardina pilchardus fish. Samples originated from the Atlantic Northeast fishing area 27 and were analysed by inductively coupled plasma optical emission spectroscopy. The aim of the study was to estimate the health impact of consumption, based on the concentrations and calculated exposure for each element. Based on the average total arsenic concentration of 3.26 ± 0.39 mg/kg in the samples, the estimated inorganic arsenic content and calculated PTWI suggested that the samples do not pose a health risk for consumers. Cadmium content of the samples was also not considered hazardous, as both the measured concentrations and PTMI values were below the specified limits. Lead concentration exceeded the maximum limit in 73% of the samples, while calculated average weekly intake was below the recommended level. The measured mercury concentrations exceeded the maximum limit in only 5% of the samples.

Comparison of metal burden in different muscle tissues of Great cormorant (Phalacrocorax carbo).

Concentrations of 12 metals (As, Ba, Cd, Co, Cr, Cu, Hg, Mn, Mo, Ni, Pb and Zn) were examined in the pectoral and thigh muscle of great cormorants (Phalacrocorax carbo). The samples were collected from Central Tisza-Jászság Nature Conservation Area in Hungary. The tissue samples were analysed by inductively coupled plasma optical emission spectroscopy (ICP-OES). The aim of the study was to examine the impacts of heavy metal pollution on the water birds, determine the concentrations of the abovementioned metals in the different muscle tissues of these wetland birds, and provide the basic materials for monitoring the environmental pollution. Among the investigated elements/metals, the detected concentrations of As, Ba, Cd, Co, Cr, Mo and Ni were below the detection limit. Higher concentration of Cu, Hg, Mn and Pb was measured in the pectoral muscle compared to the thigh muscle, but only in the case of Cu and Mn were found significant differences between the tissues. In the case of the Zn concentration, the higher value was detected in the thigh muscle. There were no statistical differences between males and females in either metal concentrations.

Biomonitoring of toxic metals in feathers of predatory birds from eastern regions of Hungary.

The aim of our study was to investigate the concentrations of toxic metals in the feathers of predatory birds in the Hortobágyi Madárpark (Bird Hospital Foundation). Samples were collected from different predatory birds originated from the eastern and north-eastern region of Hungary. Inductively coupled plasma optical emission spectrometry was used to determine the concentration of toxic metals. The mean values varied between bird species groups, their concentrations were between 0.29 ± 0.24 and 0.40 ± 0.30 mg/kg for arsenic (As), 0.09 ± 0.03 and 0.20 ± 0.18 mg/kg for cadmium (Cd), 1.15 ± 1.40 to 2.30 ± 1.52 mg/kg for lead (Pb) and 0.58 ± 0.31 to 2.19 ± 1.25 mg/kg for mercury (Hg), respectively. The measured values are not over the considered threshold values for these toxic metals and in accordance with similar concentrations of them recorded in similar species within Europe. No significant differences were found in their concentration between genders or age in the species. According to the detected concentrations of these metals, their levels accumulated in the feather of the investigated birds do not indicate the possibility of poisoning.

Heavy metals in seafood purchased from a fishery market in Hungary.

Heavy-metal content (arsenic, cadmium, lead and mercury) was investigated and evaluated in shellfish, oysters and squids. The samples were collected weekly for 20 weeks from a fishery product market in Hungary. The concentration of heavy metals was measured by Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES) analysis after sample preparation. The results were analysed statistically by one-way ANOVA method. The average concentration of arsenic (3.01 ± 1.46 mg kg-1) in shellfish was significantly higher (p < 0.001) compared to oysters (2.88 ± 1.12 mg kg-1) and squids (1.28 ± 0.52 mg kg-1). The level of mercury was below the limit of detection (0.5 mg kg-1) in each sample and there was no statistical significance in the concentrations of cadmium (p = 0.351) and lead (p = 0.412) in the species investigated. The provisional tolerable intake values were also calculated. Based on the obtained results of the heavy-metal content of the investigated samples, the seafood is considered to be safe for human consumption. However, prolonged ingestion of oysters and squids at these levels may contribute to the consumers' cadmium burden.

Monitoring of heavy metal burden in wild birds at eastern/north-eastern part of Hungary.

Concentrations of different heavy metals (As, Cd, Cr, Cu, Hg, Pb, Zn) were examined in the contour feathers of long-eared owl (Asio otus), little owl (Athene noctua), tawny owl (Strix aluco), barn owl (Tyto alba), Eurasian sparrowhawk (Accipiter nisus), rook (Corvus frugilegus), hooded crow (Corvus cornix), carrion crow (Corvus corone), common buzzard (Buteo buteo) and barn swallow (Hirundo rustica). The samples were collected from the Hortobágyi Madárpark (Bird Hospital Foundation) in Hungary. The bird species were classified into six groups based on their nourishment. Feathers were analysed by inductively coupled plasma optical emission spectroscopy (ICP-OES). The aim of our study was to determine the concentration of the above-mentioned heavy metals in the six different groups and to compare them by the groups, to find a possible connection between the concentrations and the age of birds and to get some information about the heavy metal burden of the environment. The highest As concentration was measured in little owl (0.65 ± 0.56 mg/kg). The highest Cd, Cr and Pb concentration was found in the feathers of barn swallow (0.13 ± 0.06 mg/kg; 1.69 ± 0.44 mg/kg; 5.36 ± 1.46 mg/kg), while the highest Cu and Hg concentration (65.45 ± 17.66 mg/kg; 2.72 ± 1.08 mg/kg) in sparrowhawk feathers and the highest Zn concentration in owls (157.21 ± 57.3 mg/kg). Statistically significant difference has been determined between the juvenile and adult crows in the case of Cd (p = 0.011). The higher concentration was measured in adults (0.14 ± 0.04 mg/kg) than that in juveniles (0.08 ± 0.02 mg/kg). Based on our results, the examined area is not contaminated by these heavy metals on that level, which can cause any adverse effect or poisoning in birds, so this region is safe to wildlife.

Food safety aspects of primary environmental contaminants in the edible tissues of roe deer (Capreolus capreolus).

The muscle, liver, kidney and fat samples of 20 roe deer of both sexes originating from a hunting area in central Hungary were investigated for the presence of heavy metals such as As, Cd, Hg and Pb, and their contents were evaluated for possible health risk to consumers. Both As and Hg were found at a level below the limit of detection (< 0.5 mg/kg wet weight) in all samples. The median of the measured Cd concentrations was significantly higher in both the kidney and the liver (p = 0.0011) of bucks than of does. In bucks, Cd levels exceeded the respective maximum limits laid down in the European legislation in four kidney and three muscle samples, whereas in does, the measured concentrations were below the respective limits in all samples. The detected amounts of Pb exceeded the maximum limits in the kidney of one buck and eight does, in the liver of two bucks and six does, in the muscle of six bucks and nine does, whereas in all fat tissues of both bucks and does. The concentration of Pb (p = 0.02) was significantly greater in the kidney of does compared to roebucks. Based on data obtained from the present study, the consumption of organs and tissues of the investigated roe deer could be objectionable from food-toxicological point of view and may pose risk to the high consumers of wild game due to their cadmium and lead contents.

Validation of CT doses of SPECT/CT and PET/CT hybrid devices: lessons learned.

The aim of the study was to check the validity of computed tomographic (CT) doses exhibited by SPECT/CT and PET/CT hybrid devices. Dose measurements were taken from four SPECT/CT and four PET/CT cameras commercially available from different manufacturers. A calibrated ionization chamber was placed in whole-body or head phantoms for the acquisition of CT images with clinically used parameters. Computed tomography dose index (CTDIvol) values were calculated according to the IEC 60601-2-44:1999 formula. The measured CTDIvol doses were compared with those preprogrammed by the manufacturer. In the case of the whole-body phantom, the differences between the measured and displayed values varied between -31 and +24% [European document RP162 (2012) sets up the limit for acceptance criterion as ±20%]. The head phantom data showed either an agreement between -10 and +24%, or an underestimation by two-fold. The latter seemed to be because, while preprogramming the doses, the manufacturer had used the whole-body phantom instead of a proper head phantom. The results of the work demonstrate the need for individual dosimetric calibration of every single X-ray tube. Dosimetric checks should be included in the regular quality control programmes of the SPECT/CT and PET/CT devices. Special attention should be paid to head-and-neck and paediatric protocols, in which the use of a head phantom is recommended for dose calibration.